Tagi Ramiz ALİYEV, Ali Mehraj ABBASOV, Charkaz Alamdar MAMMADOV

Genetic identification of diploid and tedraploid wheat species with RAPD markes

On farklı diploit ve tetraploit buğday türünün genetik farklılıkları RAPD markerlar ile tanımlanmıştır. Diploitlerden iki tür; Triticum boeticum, Triticum monococcum ve beş tetraploit buğday; Triticum dicoccoides var. arabicum (yabani), Triticum dicoccum var. farrum, Triticum dicoccum var. atratum, Triticum durum var. hordeiforme (Bereketli 95), Triticum durum var. leucrum (Sharq), Triticum turgidum var. albayadurum, Triticum turgidum var. salomonis ve Triticum persicum analizler için kullanılmıştır. Dendrogramda türler iki grupta toplanmıştır. Varyete T. boeticum, T. dicoccoides var. arabicum (yabani), T. dicoccum var. farrum ve T.m dicoccum var. atratum, bir grupta toplanmıştır. T. monococcum, T. turgidum var. albayadurum, T. turgidum var. salomonis ve T. persicum türleri ise diğer grupta toplanmıştır. Sekiz ürün için yapılan yapısal analizlerde aynı genotipler kullanılmış ve RAPD dendrogramı ile karşılaştırma yapılmıştır. Çalışılan aksesyonlarda genetik ve fenotipik benzerlikler arasında farklıklar bulunmuştur. Sonuçlara göre genetik olarak benzer genotiplerin fenotipik olarak farklı olabileceği söylenebilir.

Diploit ve tetraploit buğday türlerinin RAPD markerlar ile genetik tanımlanması

The genetic diversity of 10 diploid and tetraploid wheat species was estimated using random amplified polymorphic DNA (RAPD) markers. Two species from diploid [Triticum boeticum (wild), Triticum monococcum] and five from tetraploid wheats [Triticum dicoccoides var. arabicum (wild), Triticum dicoccum var. farrum, Triticum dicoccum var. atratum, Triticum durum var. hordeiforme (Bereketli 95), Triticum durum var. leucurum (Sharq), Triticum turgidum var. alboyadurum, Triticum turgidum var. salomonis and Triticum persicum] were included for the analyses. Jacard’s cluster analysis algorithm was used to determine genetic similarities. There were two main classes in dendrogram: the varieties [T. boeticum, T. dicoccoides var. arabicum, T. dicoccum var. farrum, T. dicoccum var. atratum, T .durum var. hordeiforme (Bereketli 95), T. durum var. leucurum (Sharq)] assembled in one group and the species [T. monococcum, T. turgidum var. alboyadurum, T. turgidum var. salomonis and T. persicum] in another. The same genotypes were also assessed in field conditions for structural analyses, which were carried out based on eight yield components. The dendrogram created was comparatively analyzed with the RAPD dendrogram. There were differences between genetic and phenotypic similarity of the studied accessions. Results indicated that some of genetically similar genotypes were different phenotypically.

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