Triticum monococcum tohumu, Castanea sativa tohumu ve Begonia maculata yapraklarının antibakteriyel aktivitesinin çeşitli bakteri türlerine karşı değerlendirilmesi
Bakterilerin antibiyotiklere direncini önlemek için ciddi araştırmalar var ve bilim insanları, bitkileri ve doğayı kullanarak yeni antibiyotik türevleri aramaktadırlar. Siyez buğdayı tohumları (Triticum monococcum), kestane meyvesi (Castanea sativa) ve begonya yaprakları bazı bakteri türlerine karşı antibakteriyel test için kullanılmışlardır. Siyez buğday tohumu ve Begonya yapraklarının antibakteriyel etkileriyle ilgili çalışma literatürde görülememiştir. Bu bitki yaprak ve tohumlarının antimikrobiyal etkisi Muller-Hilton besiyerinde 37 Co sıcaklıkta 48 saat süreyle incelenmiştir. İnkübasyondan sonra, tüm plakalar büyüme inhibisyon bölgeleri için gözlendi. Bu bölgelerin parametreleri milimetre cinsinden ölçüldü. Tüm testler, steril koşullar altında gerçekleştirildi ve üç kez tekrarlandı. Bu çalışmada, buğday ve kestane tohumlarının Staphylococcus aureus bakterisine karşı antibakteriyel etkisi olmadığı görülmüştür. Ancak siyez buğdayı ve kestane tohumlarının, Escherichia coli baktesine karşı önemli bir antibakteriyel etkiye sahip olduğu bulunmuştur. Buna ilaveten; Begonia maculata bitki yapraklarının E. coli, Klebsiella pneumoniae ve Pseudomonas aeroginosa bakterilerine karşı antimikrobiyal etkileri incelendi. Özellikle E. coli bakterileri her iki yaprak çeşidinden diğer bakterilere göre daha fazla etkilemiştir.
Anahtar Kelimeler:
Triticum monococcum, Castanea sativa, Escherichia coli, Staphylococcus aureus, Begonia maculata, antibakteriyel
Evaluation of antibacterial activity of Triticum monococcum seeds, Castanea sativa seeds and Begonia maculata leaves against several bacterial strains
There are serious research projects to prevent the bacteria to antibiotics. Thus, scientists are looking for new antibiotic derivatives using plants. Einkorn wheat seeds (Triticum monococcum), Chestnut seed (Castanea sativa) and Begonia leaves were used for antibacterial tests on bacterial strain. The study of antibacterial effects of Einkorn wheat seed and begonia leaves has not been seen in the literature. The antimicrobial effects of these plant leaves and seeds were investigated in Muller-Hilton media at 37 Co for 48 hours. After incubation, all plates were observed for zones of growth inhibition. The parameters of these zones were measured in millimeters. All tests were performed under sterile conditions and repeated three times. It was observed that the wheat and chestnut seeds had no antibacterial effect against Staphylococcus aureus bacteria. However, they have been found to have a significant antibacterial effect against Escherichia coli bacteria. Furthermore, antimicrobial effects of Begonia maculata leaves against E. coli, Klebsiella pneumoniae and Pseudomonas aeroginosa bacteria were investigated. E. coli bacteria were affected by the leaves more than the other bacteria. K. pneumoniae bacteria were affected less while P. aeruginosa bacteria were affected the least.
Keywords:
Escherichia coli, Triticum monococcum, Castanea sativa, Staphylococcus aureus, Begonia maculata, antibacterial,
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- Yayın Aralığı: Yılda 2 Sayı
- Başlangıç: 2018
- Yayıncı: Artvin Çoruh Üniversitesi
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