Use of N and P biofertilizers reduces inorganic phosphorus application and increases nutrient uptake, yield, and seed quality of chickpea

Leguminous crops suffer severely in soils poor in phosphorus. A 2-factor factorial experiment was conducted in a net-house to explore the effect of graded levels of P fertilizer (0, 30, and 60 kg P ha-1 or P0, P30, and P60, respectively) together with rhizobium (biological nitrogen fertilizer (BNF)) and/or phosphate-solubilizing bacteria (biological phosphorus fertilizer (BPF)) in terms of nutrient uptake, yield, and quality of chickpea (Cicer arietinum L.). Phosphorus was applied as basal dose, while seeds were treated with respective biofertilizer(s) before sowing according to the treatments [BF0 (control), BNF, BPF, and BNF+BPF]. Concerning the main effects, P60 proved superior or equivalent to P30, while among the biofertilizer treatments, BNF+BPF gave the greatest values for nutrient uptake as well as for yield and quality parameters. The interaction between P levels and biofertilizer treatments was generally significant. 30 kg P ha-1 applied with N and P biofertilizers (P30 × BNF+BPF) was the most profitable interaction for N uptake as well as for yield and quality characteristics. Compared to P60 applied alone (P60 × BF0), P30 × BNF+BPF resulted in greater N uptake (27.3%), seed yield (21.1%), and the content of seed protein (2.9%) and carbohydrate (5.6%). Furthermore, P30 × BNF+BPF was statistically equal to P60 × BNF+BPF with regard to N uptake, seed yield, and protein content as well as for most yield components. Thus, P30 × BNF+BPF saved 30 kg P ha-1 of the costly inorganic P fertilizer to achieve the greatest crop yield and quality.

Use of N and P biofertilizers reduces inorganic phosphorus application and increases nutrient uptake, yield, and seed quality of chickpea

Leguminous crops suffer severely in soils poor in phosphorus. A 2-factor factorial experiment was conducted in a net-house to explore the effect of graded levels of P fertilizer (0, 30, and 60 kg P ha-1 or P0, P30, and P60, respectively) together with rhizobium (biological nitrogen fertilizer (BNF)) and/or phosphate-solubilizing bacteria (biological phosphorus fertilizer (BPF)) in terms of nutrient uptake, yield, and quality of chickpea (Cicer arietinum L.). Phosphorus was applied as basal dose, while seeds were treated with respective biofertilizer(s) before sowing according to the treatments [BF0 (control), BNF, BPF, and BNF+BPF]. Concerning the main effects, P60 proved superior or equivalent to P30, while among the biofertilizer treatments, BNF+BPF gave the greatest values for nutrient uptake as well as for yield and quality parameters. The interaction between P levels and biofertilizer treatments was generally significant. 30 kg P ha-1 applied with N and P biofertilizers (P30 × BNF+BPF) was the most profitable interaction for N uptake as well as for yield and quality characteristics. Compared to P60 applied alone (P60 × BF0), P30 × BNF+BPF resulted in greater N uptake (27.3%), seed yield (21.1%), and the content of seed protein (2.9%) and carbohydrate (5.6%). Furthermore, P30 × BNF+BPF was statistically equal to P60 × BNF+BPF with regard to N uptake, seed yield, and protein content as well as for most yield components. Thus, P30 × BNF+BPF saved 30 kg P ha-1 of the costly inorganic P fertilizer to achieve the greatest crop yield and quality.

___

  • Aslam M, Ahmad HK, Himayatullah AM, Ahmad E, Sagoo AG, Hussain IUA, Manzoor M (2010) Nodulation, grain yield and grain protein contents as affected by rhizobium inoculation and fertilizer placement in chickpea cultivar bittle-98. Sarhad J Agric 26: 467–474.
  • Bahadur MM, Ashrafuzzaman M, Kabir MA, Choudhary MF, Majumdar AN (2002) Response of chickpea (Cicer arietinum L.) varieties to different levels of phosphorus. Crop Res 23: 293–299.
  • Chavan PG, Shinde VS, Solunke PS, Kote GM, Bhondve AA (2008) Response of sources and levels of phosphorus with and without PSB inoculation on yield, economics and uptake of soybean. Crop Res 35: 17–20.
  • Dutta D, Prohit B (2009) Performance of chickpea (Cicer arietinum L.) to application of phosphorus and biofertilizer in laterite soil. Arch Agron Soil Sci 55: 147–155.
  • Elsheikh EAE, Mohamedzein EMM (1998) Effect of bradyrhizobium, VA mycorrhiza and fertilizers on seed composition of groundnut. Ann Appl Biol 132: 325–330.
  • Fatima Z, Zia M, Chaudhary MF (2007) Interactive effect of Rhizobium strains and P on soybean yield, nitrogen fixation and soil fertility. Pak J Bot 39: 255–264.
  • Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66: 375–400.
  • Gitari JN, Mureithi JG (2003) Effect of phosphorus fertilization on legume nodule formation and biomass production in Mount Kenya Region. East Afr Agric For J 69: 183–187.
  • Gupta SC, Sukhlal N, Paliwal KK, Namdeo S (1998) Effect of P levels and microbial inoculants on symbiotic traits, N and P uptake, quality and yield of rain fed chickpea (Cicer arietinum L.). In: Proceedings of the 3rd European Conference on Grain Legumes: Opportunities for High Quality, Healthy and Added-value Crops to Meet European Demands. Paris, France: European Association for Grain Legume Research, pp. 418– 4
  • Jain PC, Kushawaha PS, Dhakal US, Khan H, Trivedi SM (1999) Response of chickpea (Cicer arietinum L.) to phosphorus and biofertilizer. Legume Res 22: 241–244.
  • Jat RS, Ahlawat IPS (2006) Direct and residual effect of vermicompost, biofertilizers and phosphorus on soil nutrient dynamics and productivity of chickpea-fodder maize sequence. J Sustain Agric 28: 41–54.
  • Khanda CM, Mahapatra AK, Misra PK (2001) Response of rice bean (Vigna umbellata) to row spacing and phosphorus under rainfed condition. Ann Agric Res 22: 481–484.
  • Kumar R, Chandra R (2008) Influence of PGPR and PSB on Rhizobium leguminosarum Bv. viciae strain competition and symbiotic performance in lentil. World J Agric Sci 4: 297–301. Kumar A, Kushwaha HS (2006) Response of pigeonpea to sources and levels of phosphorus under rain-fed condition. Indian J Agron 51: 60–62.
  • Lindner RC (1944) Rapid analytical methods for some of the more common inorganic constituents of the plant tissues. Plant Physiol 19: 76–89.
  • Lowry OH, Rosebrough NS, Farrand AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193: 263–275.
  • Mayz J, Campos F, Vallandears N (2003) Evaluation of rhizobial (Rhizobium spp.) isolates for their effects on cowpea (Vigna unguiculata L.) growth and yield. Tropic Agric 80: 36–40.
  • Meena LR, Singh RK, Gautam RC (2003) Yield and nutrient uptake of chickpea (Cicer arietinum) as influenced by bacterial inoculation. Legume Res 26: 109–112.
  • Naeem M, Khan MMA (2005) Growth, physiology and seed yield of Cassia tora (syn. Cassia obtusifolia) as affected by phosphorus fertilization. J Med Arom Plant Sci 27: 4–6.
  • Nishita G, Joshi NC (2010) Growth and yield response of chickpea (Cicer arietinum) to seed inoculation with Rhizobium sp. Nat  Sci 8: 232–236. 
  • Novozamsky I, Houba VJG, van Eck R, van Vark W (1983) A novel digestion technique for multi-element plant analysis. Comm Soil Sci Plant Anal 14: 239–248.
  • Ogola AH, Odhiambo GD, Okalebo JR, Muyekho FN (2012) Influence of phosphorus on selected desmodium growth and nodulation parameters. ARPN J Agric Biolo Sci 7: 294–301.
  • Patra RK, Pant LM, Pradhan K (2012) Response of soybean to inoculation with rhizobial strains: effect on growth, yield, N uptake and soil N status. World J Agric Sci 8: 51–54.
  • Rorison IH, Spencer RE, Gupta PL (1993) Chemical analysis. In: Hendry GAE, Grime JP, editors. Methods in Comparative Plant Ecology. New York, NY, USA: Chapman and Hall, pp. 156–161.
  • Sadasivam S, Manickam A (2008) Biochemical Methods. 3rd ed. New Delhi, India: New Age International Ltd.
  • Selvakumar G, Lenin M, Thamizhiniyan P, Ravimycin T (2009) Response of biofertilizers on the growth and yield of blackgram (Vigna mungo L.). Recent Res Sci Tech 1: 169–175.
  • Selvakumar G, Reetha S, Thamizhiniyan P (2012) Response of biofertilizers on growth, yield attributes and associated protein profiling changes of blackgram (Vigna mungo L. Hepper). World App Sci J 16: 1368–1374.
  • Singh RP, Gupta SC, Yadav AS (2008) Effect of levels and sources of phosphorus and PSB on growth and yield of blackgram (Vigna mungo L. Hepper). Legume Res 31: 139–141.
  • Singh JP, Tarafdar JC (2001) Improvement in yield and quality of mungbean (Vigna radiata L.) by rhizobium inoculation. Ann Agric Res 22: 360–364.
  • Taiz L, Zeiger E (2006) Plant Physiology. 4th ed. Sunderland, MA, USA: Sinauer Associates.
  • Tewari S, Pal RS (2005) Effect of phosphorus and potassium on yield, quality, economics and balance studies of soybean. Res Crops 6: 446–447.
  • Tran TNS, Cao ND, Truong TMG (2006) Effect of bradyrhizobia and phosphate solubilizing bacteria application on soybean in rotational system in the Mekong Delta. Omonrice 14: 48–57.
  • Verma LN (1993) Biofertiliser in agriculture. In: Organics in Soil Health and Crop Production. (Ed. P K Thampan), Peekay Tree Crops Development Foundation, Cochin, India, pp. 152–183.
  • Walley FL, Kyei-Boahen S, Hnatowich G, Stevenson C (2005) Nitrogen and phosphorus fertility management for desi and kabuli chickpea. Can J Plant Sci 85: 73–79.
Turkish Journal of Agriculture and Forestry-Cover
  • ISSN: 1300-011X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents

Filiz Özkan ÇAKICI, Ali SEVİM, Zihni DEMİRBAĞ, İsmail DEMİR

Utilization of related wild species (Echinacea purpurea) for genetic enhancement of cultivated sunfower (Helianthus annuus L.)

Boris KRAPTCHEV, Roumiana IVANOVA VASSILEVSKA, Nina GEORGIEVA, Ivan ILIEV, Ira STANCHEVA, Maria GENEVA

Potential of Moringa oleifera L. as livestock fodder crop: a review

Wasif NOUMAN, Shahzad Maqsood Ahmed BASRA, Muhammad Tahir SIDDIQUI

Artifcial neural networks in online semiautomated pest discriminability: an applied case with 2 Trips species

Pavol PROKOP, Eladia Maria PENA MENDEZ, Halina KUCHARCZYK, Jaromir VANHARA, Josef HAVEL, Martina DORICOVA, Peter FEDOR

Oxidative stress and antioxidant defense mechanism in mung bean seedlings afer lead and cadmium treatments

Meher HASSAN, Simeen MANSOOR

Transferability of SSR markers from distantly related legumes to Glycyrrhiza species

Mustafa ERAYMAN, Emre İLHAN, Yelda GÜZEL, Abdil Hakan EREN

Extractability and phytoavailability of cadmium in Cd-rich pedogenic soils

Kürşad ÖZBEK, Nesime CEBEL, İlhami ÜNVER

Long-term water balance of a bare soil with slope in Chuncheon, South Korea

Mehmet AYDIN, Yeong-Sang JUNG, Jae E. YANG, Hyun-il LEE

Genotypic-unspecifc protocols for the commercial micropropagation of Eucalyptus grandis × nitens and E. grandis × urophylla

Maria Paula WATT

Efects of diferent organic materials on forage yield and quality of silage maize (Zea mays L.)

Alpaslan KUŞVURAN, Veyis TANSI, Ahmet DEMİRBAŞ, Recep İrfan NAZLI, İlker İNAL