Oxidative stress and antioxidant defense mechanism in mung bean seedlings after lead and cadmium treatments

This research was an attempt to study the effect of heavy metals lead and cadmium (0.05 mM and 0.3 mM) on growth and antioxidant enzymes of seedlings of 2 mung bean genotypes (NM 19-19 and Azri mung-2006). Results revealed that germination percentage and seedling length decreased when compared with the control for both genotypes. However, seedling length and germination percentage was better in NM 19-19 as compared to Azri mung-2006. Elevated levels of protein were observed under metal stress in both genotypes. Heavy metals induced oxidative stress in plants, causing membrane injury as observed by enhanced level of malondialdehyde (MDA) contents. Increase in antioxidant enzymes activity was detected for guaiacol peroxidase (GPX) and catalase (CAT). However, ascorbate (APX) activity decreased under stress for both genotypes. We observed more MDA content and GPX and APX activity in Azri mung-2006 as compared to NM 19-19 when high concentrations of Pb and Cd were added. This revealed that NM 19-19 was tolerant whereas Azri mung-2006 was sensitive to Pb and Cd. It was further noticed that Cd imposed a more deleterious effect than Pb on both genotypes.

Anahtar Kelimeler:

Key words: Ascorbate peroxidase, cadmium, catalase, guaiacol peroxidase, lead, malondialdehyde

Oxidative stress and antioxidant defense mechanism in mung bean seedlings after lead and cadmium treatments

Keywords:

Key words: Ascorbate peroxidase, cadmium, catalase, guaiacol peroxidase, lead, malondialdehyde,

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Aebi H (1984). Catalase in vitro. Method Enzymol 105: 121–126. Bhardwaj P, Ashish KC, Prasad P (2009). Effect of enhanced lead and cadmium in soil on physiological and biochemical attributes of Phaseolus vulgaris. Nature Sci 8: 63–75.
Cvetanovska L, Klincharska-Jovanovska I, Dimeska G, Srbinoska M, Cvetanovska A (2010). Anatomic and physiological disorder after intoxication with heavy metals in tobacco (Nicotiana tabacum L.). Second Balkan Conference on Biology, Plovdiv. Biotechol & Biotechnol Equip 24: 4–9
Das P, Samantaray S, Rout GR (1997). Studies on cadmium toxicity in plants: a review. Environ Pollut 98: 29–36.
Dey SK, Dey J, Patra S, Pothal D (2007). Changes in the antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed to cadmium and lead stress. Braz J Plant Physiol 19: 53–60.
El-Beltagi HS, Mohamed A, Rashed M (2010). Response of antioxidative enzymes to cadmium stress in leaves and roots of radish (Raphanus sativus L.). Not Sci Biol 2: 76–82.
Everse J, Johnson MC, Marini MA (1994). Peroxidative activities of haemoglobin derivatives. In: Everse J, Vandegriff KD, Winslow RM. editors. Methods in Enzymology. London: Academic Press, pp. 547–561.
Ganesh KS, Baskaram L, Rajasekaram S, Sumathi K, Chidambaram ALA, Sundaramoorthy P (2008). Cadmium stress induced alterations in biochemical and enzyme metabolism in aquatic and terrestrial plants. Colloid Surface B 63: 159–163.
Ghani A (2010). Effect of cadmium toxicity on the growth and yield components of mung bean [Vigna radiata (L.) Wilczek]. World Appl Sci J 8: 26–29.
Gonçalves JF, Becker AG, Cargnelutti D, Tabaldi AL, Pereira LB, Battisti V, Spanevello RM, Morsch VM, Nicoloso FT, Schetinger MRC (2007). Cadmium toxicity causes oxidative stress and induces response of the antioxidant system in cucumber seedlings. Braz J Plant Physiol 19: 223–232.
Heath RL, Packer L (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid and peroxidation. Arch Biochem Biophys 125: 189–198.
Jiang Y, Huang B (2001). Effects of calcium on antioxidant activities and water relations associated with heat tolerance in coolseason grasses. J Exp Bot 52: 341–349.
Kaur G, Singh HP, Batish DR, Kohli RK (2012a). A time course assessment of changes in reactive oxygen species generation and antioxidant defense in hydroponically grown wheat in response to lead ions (Pb2+). Protoplasma 249: 1091–1100.
Kaur G, Singh HP, Batish DR, Negi A, Mahajan P, Rana S, Kohli RK (2012b). Arsenic (As) inhibits radicle emergence and elongation in Phaesolus aureus by altering starch-metabolizing enzymes vis-à-vis disruption of oxidative metabolism. Biol Trace Elem Res 146: 360–368.
Kuo MC, Kao CH (2004). Antioxidant enzyme activities are up regulated in response to cadmium in sensitive, but not in tolerant, rice (Oryza sativa L.) seedlings. Bot Bull Acad Sinica 45: 291–299.
Laspina NV, Groppa MD, Tomaro ML, Benavides MP (2005). Nitric oxide protects sunflower leaves against Cd-induced oxidative stress. Plant Sci 169: 323–330.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951). Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275.
Mahmood T, Islam KR, Muhammad S (2007). Toxic effects of heavy metals on early growth and tolerance of cereal crops. Pak J Bot 39: 451–462.
Mroczek-Zdyrska M, Wojcik M (2012). The influence of selenium on root growth and oxidative stress induced by lead in Vicia faba L. minor plants. Biol Trace Elem Res 147: 320–328.
Nakano Y, Asada K (1981). Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22: 867–880.
Namjooyan S, Khavari-Nejad R, Bernard F, Namdjoyan S, Pir H (2012). The effect of cadmium on growth and antioxidant responses in the safflower (Carthamus tinctorius L.) callus. Turk J Agric For 36: 145–152.
Salt DE, Blaylock M, Kumar NPBA, Dushenkoz V, Ensley BD, Chet I, Raskin I (1995). Phytoremidiation – A novel strategy for removal of toxic metals from the environmental with plants. Biotech 13: 468–474.
Sanita di Toppi L, Gabbrielli R (1999). Response to cadmium in higher plants. Environ Exp Bot 41: 105–130. Schützendübel A, Polle A (2002). Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot 53: 1351–1365.
Shan YJ, Pu LJ, Wu J, Tian WH, Zhang MP (2009). Effects of lead ion on the mung bean seedling root. In: 3rd International Conference on Bioinformatics and Biomedical Engineering, pp. 1-4.
Sheppard SC, Grant CA, Sheppard ML, De Jong R, Long J (2009). Risk indicator for agricultural inputs of trace elements to Canadian soils. J Environ Qual 38: 919–932.
Singh HP, Kaur G, Batish DR, Kohli RK (2011). Lead (Pb)-inhibited radicle emergence in Brassica campestris involves alterations in starch-metabolizing enzymes. Biol Trace Elem Res 144: 1295–1301.
Solanki R, Anju, Poonum, Dhankar R (2011). Zinc and copper induced changes in physiological characteristics of Vigna mungo (L). J Environ Biol 32: 747–751.
Steel RGD, Torrie JH (1980). Principles and Procedures of Statistics. New York: McGraw Hill.
Verma S, Dubey RS (2003). Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 164: 645–655.
Vranova E, Inze D, Van Breusegem F (2002). Signal transduction during oxidative stress. J Exp Bot 53: 1227–1236.