Salih MADEN, Aynur Çolak TÜMBEK, Yakup Zekai KATIRCIOĞLU, Pınar ÖZEREN, Rıza KAYA

Sensitivity of Cercospora beticola populations in Turkey to flutriafol, mancozeb, and fentin acetate

Cercospora beticola’ nın neden olduğu Şeker Pancarı Yaprak Lekesi hastalığının Türkiye’ de kullanılan fungisitlere karşı dayanıklılığı petri kaplarında mycelial gelişme deneyleri ile 2005-2006 yıllarında 6 şeker pancarı fabrikası üretim alanından elde edilen 100 fungus izolatı kullanılarak araştırılmıştır. Bunun için flutriafol, mancozeb ve fentin asetat Aspergillus Complete Medium (ACM)’ a 0.0, 0.05, 0.1, 0.5, 1.0, 5.0, 10.0 μg $mL^ {-1}$ dozlarda katılmış ve miselyal gelişme ölçülmüştür. Şeker pancarı üretim alanlarından elde edilen tüm izolatların flutriafol için $ED _{50}$ değerleri 0.04 ile > 10.0 μg $mL^ {-1}$ arasında değişmiştir. Bu fungiside karşı tolerans, bu hastalığın gelişmesi için uygun iklim koşullarını içeren ve hastalığın şiddetli olduğu iki bölgede, Adapazarı ve Çarşamba, belirgin derecede yüksek bulunmuştur. Bu iki bölgede; izolatların sırasıyla %74.1, %55.5, %81.2 ve %68.7’ i, fungusun gelişmesini engellediği kabul edilen 5 ve 10 μg $mL^ {-1}$ flutriafol dozunda gelişmiştir. Diğer 4 bölgede, bu fungisite tolerans bu kadar yüksek oranlarda değildi. Mancozeb’ e tolerans tümbölgelerde görülmüş ve tolerant izolatların yüzdesi Çarşamba’ da en düşük (%6) ve Susurluk bölgesinde en yüksek (%47.3) olmuştur. Fentin asetat’ a karşı tolerans değerlendirilme kriterine bağlı olarak değişiklik göstermiştir.

Türkiye’de Cercospora beticola popülasyonlarının flutriafol, mancozeb ve fentin acetate’a duyarlılıkları

The sensitivity of sugar beet leaf spot disease caused by Cercospora beticola to fungicides used in Turkey was determined using amycelial growth assay in petri dishes with 100 fungal isolates. The isolates were obtained fromsamples collected from 6 sugar beet production areas in 2005 and 2006. Flutriafol, mancozeb, and fentin acetate were added to Aspergillus completemedium(ACM) at the rates of 0.0, 0.05, 0.1, 0.5, 1.0, 5.0, and 10.0 μg$mL^ {-1}$, and thenmycelial growth wasmeasured. $ED _{50}$ values of flutriafol for all the isolates fromthe sugar beet production areas ranged from0.04 to >10.0 μg $mL^ {-1}$. Tolerance to this fungicide was very high in 2 regions (Adapazarı and Çarşamba) where the disease is very prevalent due to climatic conditions. In these 2 areas 74.1%, 55.5%, 81.2%, and 68.7% of the isolates grew with 5 and 10 μg $mL^ {-1}$ of flutriafol, respectively (1 μg $mL^ {-1}$ flutriafol is considered inhibitory to the growth of the fungus). In the other 4 regions included in the study, tolerance was not as high. Tolerance to mancozeb developed in all the regions; however, the percentage of tolerant isolates was lowest in Çarşamba (6%) and highest in Susurluk (47.3%). Tolerance to fentin acetate varied according to the criteria used for evaluation.

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Briere SC, Franc GD, Kerr ED (2001) Fungicide sensitivity characteristics of Cercospora beticola isolates recovered from the high plains of Colorado, Montana, Nebraska, and Wyoming 1. Benzimidazole and triphenyltin hydroxide. J of Sugar Beet Res 38: 111-120.
Bugbee WM (1995) Cercospora beticola tolerant to triphenyltin hydroxide. J Sugar Beet Res 32: 167-174.
Calpouzos L, Stallknecht GF (1966) Phototropism by conidiophores of Cercospora beticola. Phytopathology 56: 702-704.
Giannopolitis CN (1978) Occurrence of strains of Cercospora beticola resistant to triphenyltin fungicides in Greece. Plant Dis Rep 62: 205-208.
Holtschulte B (2000) Cercospora beticola – worldwide distribution and incidence. In Cercospora beticola Sacc. biology and agronomic influence and control measures in sugar beet. Advances in Sugar Beet Research 2: 5-16.
Ioannidis PM, Karaoglanidis GS, Ioannidis PI, Karadimos DA (2002) Sensitivity profiles of Cercospora beticola populations to several fungicide groups in Greece. Resistant Pest Management Newsletter 11: 36-39.
Karadimosa DA, Karaoglanidis GS, Tzavella-Klonaric K (2005) Biological activity and physical modes of action of the Qo inhibitor fungicides trifloxystrobin and pyraclostrobin against Cercospora beticola. Crop Protection 24: 23-29.
Karaoglanidis GS, Ioannidis PM, Thanassoulopoulos CC (2001) Influence of fungicide spray schedules on the sensitivity of Cercospora beticola to the sterol demethylation-inhibiting fungicide Flutriafol. Crop Protection 20: 941-947.
Karaoglanidis GS, Karadimos DA, Ioannidis PM (2003a) Detection of resistance to sterol demethylation-inhibiting (DMI) fungicides in Cercospora beticola and efficacy of control of resistant and sensitive strains with flutriafol. Phytoparasitica 31: 373-380.
Karaoglanidis GS, Karadimosa DA, Ioannidis PM, Ioannidis PI (2003b) Sensitivity of Cercospora beticola populations to fentin-acetate, benomyl and flutriafol in Greece. Crop Protection 22: 735-740.
Meriggi P, Rosso F, Ioannidis PM, Ayala Garcia J (2000) Fungicide treatments against Cercospora leaf spot in sugar beet. In Cercospora beticola Sacc. Biology and agronomic influence and control measures in sugar beet. Advances in sugar beet research 2: 77-102.
Mohamed FR, Smith K, Larry J (2005) Evaluating fungicides for controlling Cercospora leaf spot on sugar beet. Crop Protection 24: 79-86.
ÖzgürOE (2003) Sugar beet diseases in Turkey. General Directory of Turkish Sugar Factories Inc. Publication, No 219, 192 p.
Piszczek J, Czekalska A (2006) Resistance of Cercospora beticola Sacc. to fungicides used against this pathogen in Poland. Progress in Plant Protection 46: 375-379.
Shane WW, Teng PS (1992) Impact of Cercospora leaf spot on root weight, sugar yield and purity of Beta vulgaris. Plant Disease 76: 812-820.
Weiland JJ (2001). A survey for the prevalence and distribution of Cercospora beticola tolerant to triphenyltin hydroxide and mancozeb and resistant to thiophanatemethyl in 2000. 2000 Sugarbeet Res Ext Rep 31: 266-271.
Weiland JJ, Halloin JM (2001) Benzimidazole resistance in Cercospora beticola sampled from sugar beet in Michigan, USA Can J Plant Pathol 23: 78-82.