Harun BAYRAKTAR, Salih MADEN, F. Sara DOLAR

Mating type groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the cusal agent of chickpea blight in central anatolia

Orta Anadolu bölgesindeki 6 farklı nohut ekim alanından toplanan 45 Ascochyta rabiei (teleomorph: Didymella rabieî) izolatının mating type gruplarının dağılımı laboratuar koşullarında araştırılmıştır. Nohut gövde parçalan üzerinde pseudothecium gelişimi ve ascospor üretimi 8 ± 1 °C sıcaklık ve % 85 nispi nem içeren koşullarda 5-6 haftalık inkübasyon periyodundan sonra gözlenmiştir. Kayseri ve Sivas illeri hariç diğer tüm illerde her iki mating type grubu da bulunmuştur. Test edilen izolatlann büyük bir kısmı Mat 1-1 (% 57.8) içerisinde yer alır iken % 42.2' si ise MAT 1-2 grubu içinde yer almıştır. Ayrıca multiplex PCR (primer: Coml, Tail 5 ve SP21) metodu kullanılarak A. rablel nin mating type grupları birbirinden hızlı olarak ayırt edilmiştir. Mating type gruplarının klasik yöntemler kullanılarak yapılan ayırımı ile uyumlu olarak MAT 1-1 izolatlarından 460 bp, MAT 1-2 izolatlarından ise yaklaşık 700 bp büyüklüğünde spesifik fragmentler çoğaltılmıştır.

Nohutlarda yanıklık etmeni Ascochyta rabiei' nin (Teleomorph: Didymella rabiei) Orta ANadolu Bölgesindeki mating type grupları

The stribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production .were observed on chickpea stem pieces that were incubated in 8 ± 1 °C and 85% relative humidity. Both mating types were found in all the provinces, except for Kayseri and Sivas. The majority of the isolates belonged to Mat 1-1 (57.8%) .and the others (42.2%) to MAT 1-2. Additionally, the mating types of A. rabiei were differentiated from each other using the multiplex PCR method with the primers Coml, Tail 5, and SP21. MAT 1-1 isolates were amplified to approximately a 460 bp fragment and MAT 1-2 isolates to a 700 bp fragment, in accordance with the discrimination of mating type groups with conventional methods.

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