Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae
Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry1 gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands. In addition to the 270-bp band, the standard strain and isolate I47 yielded 180-bp PCR products. The isolates and standard strain were toxic to Ephestia kuehniella Zeller and Plodia interpunctella Hubner larvae. Insecticidal activity of isolates I36, I37, and I40 against Ephestia kuehniella was higher than that of the standard strain when 106 spore-crystals mL-1 was used. LC99 values for isolates I36, I37, and I40 were 6.46, 6.28, and 6.24 for E. kuehniella, respectively, and these isolates were also effective against Plodia interpunctella larvae. LC99 values for isolates I36 and I40 were 6.69 and 5.07 for P. interpunctella, respectively. The results clearly show that isolates I36, I37, and I40 were effective at lower doses, as compared to the others.
Anahtar Kelimeler:
Key words: Bacillus thuringiensis, cry1 primer, Ephestia kuehniella, Plodia interpunctella
Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae
Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry1 gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands. In addition to the 270-bp band, the standard strain and isolate I47 yielded 180-bp PCR products. The isolates and standard strain were toxic to Ephestia kuehniella Zeller and Plodia interpunctella Hubner larvae. Insecticidal activity of isolates I36, I37, and I40 against Ephestia kuehniella was higher than that of the standard strain when 106 spore-crystals mL-1 was used. LC99 values for isolates I36, I37, and I40 were 6.46, 6.28, and 6.24 for E. kuehniella, respectively, and these isolates were also effective against Plodia interpunctella larvae. LC99 values for isolates I36 and I40 were 6.69 and 5.07 for P. interpunctella, respectively. The results clearly show that isolates I36, I37, and I40 were effective at lower doses, as compared to the others.
Keywords:
Key words: Bacillus thuringiensis, cry1 primer, Ephestia kuehniella, Plodia interpunctella,
- ISSN: 1300-011X
- Yayın Aralığı: Yılda 6 Sayı
- Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler
Commercially suitable pectin methylesterase from Valencia orange peels
Şebnem ŞİMŞEK, Ahmet YEMENİCİOĞLU
The effects of ethanol-dissolved propolis on the storage of grapefruit cv. Star Ruby
Ahmet Erhan ÖZDEMİR, Elif Ertürk ÇANDIR, Mustafa KAPLANKIRAN, Emine Mine SOYLU
Ekrem Lütfi AKSAKAL, Taşkın ÖZTAŞ
Organic materials: sources of nitrogen in the organic production of lettuce
Maja MANOJLOVIC, Ranko CABILOVSKI, Martina BAVEC