MAHMUT KAPLAN, Mustafa Mücahit YILMAZ, Serap KÖPRÜ, Hürmet GÖZELLE, Gamze MUHDEREM, Rabia USLU

Sıvı/Katı Oranı ve Etanol Konsantrasyonuna Göre Karahindiba (Taraxacum officinale) Tentürünün Verim ve Biyoaktivitesindeki Değişimin İncelenmesi

Bu çalışmada, bir hidroalkolik ekstrakt tipi olan tentür üretimi amacıyla karahindiba (Taraxacum officinale) bitkisi kullanılmış ve son ürün kalitesini artırmaya yönelik olarak üretim şartlarını optimize etmek üzere proses faktör seviyelerinin belirlenmesi amaçlanmıştır. Bu amaçla, optimizasyon işlemi için tepki yüzey yöntemi tabanlı merkezi tümleşik tasarım dizaynından faydalanılmıştır. Örneklerin hazırlanması amacıyla sıvı/katı oranı ve etanol konsantrasyonu faktör olarak seçilmiştir. Örneklerin biyoaktif özelliklerinin belirlenmesi amacıyla toplam fenolik madde miktarı, antiradikal kapasite ve EC50 değeri ile verim özellikleri hesaplanmıştır. Sıvı/katı oranı ve etanol konsantrasyonunun örneklerin toplam fenolik madde miktarı üzerinde oldukça etkili olduğu görülmüştür. Örneklerin toplam fenolik madde miktarı değerleri 605-1754 mg GAE/L aralığında, EC50 değerleri 9.80-199.2 aralığında, ekstrakt verimleri ise %2.32-6.49 aralığında değişim göstermiştir. Bitki örneklerinde sıvı miktarının artışı toplam fenolik madde miktarını artırırken ekstrakt veriminde düşüşe yol açmıştır. Optimizasyon işlemi sonucunda en yüksek toplam fenolik madde miktarının %57 etanol konsantrasyonunda ve 1:4 katı/sıvı oranında elde edilebileceği sonucuna ulaşılmıştır.

Anahtar Kelimeler:

Karahindiba, tentür, biyoaktivite, optimizasyon, tepki yüzey yöntemi

Investigation of the Change in Yield and Bioactivity of Dandelion (Taraxacum officinale) Tincture Depending on Liquid/Solid Ratio and Ethanol Concentration

In this study, dandelion (Taraxacum officinale) plant was used for the production of tincture, a hydroalcoholic extract type, and it was aimed to determine process factor levels for the optimization of the production conditions in order to increase the final product quality. For this purpose, central composite rotatable design based on response surface methodology was utilized for the optimization process. The liquid / solid ratio and the ethanol concentration were chosen as factors for the preparation of the samples. In order to determine bioactive properties of samples, total phenolic content, antiradical capacity (EC50) value and extract yield properties were determined. Liquid / solid ratio and ethanol concentration were found to be very effective over the total amount of phenolic materials. The total phenolic content values of the samples ranged from 605-1754 mg GAE/L, EC50 values ranged from 9.80-199.2, and extract yields ranged from 2.32-6.49%. Increasing the amount of liquid for herbs increased the total phenolic content, which led to a decrease in the yield of the extract. The result of the optimization process showed that the highest total phenolic content could be achieved in a 1: 4 solid / liquid ratio at a concentration of 57% ethanol.

Keywords:

Dandelion, tincture, bioactivity, optimization, response surface methodology,

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