Lyuben ZAGORCHEV, Plamena KAMENOVA, Borislav ASSENOV, Nabil ABUMHADI, Elena TODOROVSKA, Mariela ODJAKOVA

Profile of antioxidant enzymes in two Bulgarian barley cultivars at early growth stage, differing in salt stress response

Anahtar Kelimeler:

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Profile of antioxidant enzymes in two Bulgarian barley cultivars at early growth stage, differing in salt stress response

Soil salinization is among the major crop yield limiting factors in contemporary agriculture. Excessive irrigation and climate changes are among the causes for increased salinity in previously unaffected areas, including the Balkan Peninsula. The development of salt tolerant cultivars of existing crops is essential for meeting the growing food production necessity and for utilization of salinized agricultural areas. Marker assisted selection is among the promising approaches for fast and efficient screening of newly developed cultivars for tolerance to various stresses. The identification of genetic and protein markers for stress tolerance is an essential tool for accelerating of breeding programs and development of modern agriculture. Along with osmotic stress and ion toxicity, high NaCl concentrations negatively affect plant growth and development by promoting burst in reactive oxygen species formation. Antioxidative systems are essential for overcoming of this negative effect. In the present study two Bulgarian barley cultivars – Bozhin and IZ Bori were studied. Four days old seedlings were evaluated for their ability to grow at 0.15 and 0.3 M NaCl and proteins were isolated from roots and leaves. Antioxidant enzymes: peroxidases, ascorbate peroxidases, catalases, superoxide dismutases and glutathione reductases were studied. Enzymatic profiles obtained by zymographic analyses after electrophoretic separation showed several isoforms associated with salt stress response and salt stress tolerance. Further analyses and comparison of zymographic to genetic and metabolomic data would further reveal differences in the two cultivars and establish appropriate molecular markers for salt tolerance

Keywords:

antioxidative enzymes, reactive oxygen species, salt stress zymographic analysis,

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