Muharrem ERGUN, Zahide SÜSLÜOĞLU, Aydın Şükrü BENGÜ

FTIR Spektroskopisi Kullanarak Sideritis pisidica Boiss. & Heldr. Bitki Aksamlarındaki Yapısal Farklılığın Araştırılması

Fourier dönüşümlü kızılötesi spektroskopisi (FTIR) kısa sürede ve kolay uygulanabilmesi nedeni ile fizik bilim insanlarından bahçe bitkileri bilim insanlarına kadar birçok araştırmacının başvurduğu değişik organik ve inorganik maddelerin yapılarını araştırmak amacıyla kullanılan yenilikçi bir yöntemdir. Bu çalışmada, FTIR, Türkiye’de doğal olarak yetişen yabani Sideritis pisidica Boiss. & Heldr. bitki aksamlarındaki farklılığı ortaya koymak için kullanılmıştır. Bitkinin gövdesi, yaprakları ve çiçeklerinde bulunan Na, Cr, Zn, Fe, Mg, Mn, Co, Cu ve Ni gibi bazı mineraller ile protein, yağ, çözünür şeker ve nem içeriği, FTIR spektrumlarının ana yapısal içerikleri tahmin edip edemeyeceği amacıyla analiz edilmiştir. Tahmin edildiği gibi, çoğu minerallerin miktarı yaprakta yüksek miktarda bulunmuş ve yaprağı sırası ile çiçek ve gövde takip etmiştir. Bununla birlikte çiçek, yaprak ve gövdeye göre Ca, Cr ve Zn mineralleri bakımından daha zengin bir içeriğe sahip olmuştur. Yaprak, gövde ve çiçeğe göre daha fazla protein içermiştir. Toplam proteinin aksine, ham yağ içeriği gövdede en yüksek bulunmuş ve gövdeyi sırası ile çiçek ve yaprak takip etmiştir. Pik içeriği dikkate alındığında, FTIR spektrumları Sideritis pisidica Boiss. & Heldr. bitkisinin gövde, yaprak ve çiçekte bulunan protein veya toplam çözünür şeker içeriklerindeki farklığı açıkça ortaya koymuştur. Fakat spektrumlar bitki aksamlarındaki ham yağ veya nem içeriğini tahmin edememiştir.

Anahtar Kelimeler:

Bitkisel çay, dağ çayı, FTIR, Sideritis spp, Dedegöl Dağı

Studying Structural Differentiation of Plant Parts of Sideritis pisidica Boiss. & Heldr. Using FTIR Spectroscopy

Fourier Transform Infrared Spectroscopy (FTIR) is a novel tool for exploring compositional structures of diverse organic or inorganic compounds employed by many researches from physicists to horticulturists due to its time-saving and easy-to-use application. FTIR was performed to differentiate structural differentiation of plant parts of wild-grown Sideritis pisidica Boiss. & Heldr. from Turkey. Some minerals Na, Cr, Zn, Fe, Mg, Mn, Co, Cu and Ni; protein, lipid, soluble sugar and moisture contents in the stalk, leaf and blossom of the plant were analyzed whether the FTIR spectra may predict major structural compositions. As expected most of the minerals were presented higher amount in the leaf followed by the blossom and stalk. The blossom was however found be richer in Ca, Cr, Ni and Zn than leaf and stalk. Total protein contents of the leaf were higher than those of the stalk or the leaf. Crude lipid amounts were however more prominent in the stalk followed by the blossom and leaf respectively, unlike the total protein contents. The blossom was found be richer in moisture content than the leaf and stalk followed, respectively. The FTIR spectra of the plant parts clearly revealed that protein and total soluble sugar contents are varied in stalk, leaf or blossom of Sideritis pisidica Boiss. & Heldr. interpreted from the peak intensities. The spectra however did not reveal the crude lipid or moisture contents of the plant parts.

Keywords:

Herbal tea, Mountain tea, FTIR, Sideritis spp, Dedegöl Mountain,

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