Detection of Viruses Infecting Sweet Cherry Cultivars and Elites at the Institute of Agriculture-Kyustendil, Bulgaria

Anahtar Kelimeler:

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Detection of Viruses Infecting Sweet Cherry Cultivars and Elites at the Institute of Agriculture-Kyustendil, Bulgaria

То аssess the virus infections of new and commercial sweet cherry cultivars and elites, surveys were carried out in two collection orchards at the Institute of Agriculture – Kyustendil located in the Southwest Bulgaria. In the spring of 2012 and 2013 a total of 165 samples were individually collected and tested by DAS-ELISA for Prunus necrotic ringspot virus (PNRSV), Prune dwarf virus (PDV), Plum pox virus (PPV), Apple chlorotic leafspot virus (ACLSV), Cherry leaf roll virus (CLRV) and Raspberry ringspot virus (RpRSV). A total of 24.2 % of ELISA-tested samples was infected at least by single virus. PDV (13.9 %) was the most prevalent followed by ACLSV (5.4 %) and PNRSV (4.8 %). The other viruses were no presented. The infection was found in both cultivars and elites. These results demonstrated that measures should be taken to avoid using infected parents for crossing

Keywords:

Bulgaria, Kyustendil, sweet cherry, cultivars, viruses ELISA,

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