HATİCE ÖĞÜTCÜ, Ömer Faruk ALGUR

Yabani Baklagil Bitkilerinden, Mikrobiyal Gübre Olarak Kullanılan Rhizobium Spp. Bakterilerinin İzolasyonu

Araştırmamızda kullanılan yabani baklagil bitkileri ( Medicago sativa, M.lupulina ve M. varia) Erzurum iline ait yüksek rakımlı (2000-2500m) bölgelerden (Palandöken dağı, Alibaba dağı, Turnagöl dağı, Hasanbaba dağı, Eğerli dağı, Yıldırım dağı, Çubuklu dağı, Deveboynu mevkii, Kayakyolu mevkii, Telsizler tepesi ve Dumlu tepesi) Haziran ve Temmuz aylarında toplandı. Bu bitkilerden elde edilen nodüller steril edilerek YMA (Yeast Mannitol Agar) plaklarına çizgi ekim yapıldı ve petriler 28+1 ºCde 3-5 gün süreyle inkübasyona bırakıldı. İnkübasyon süresi sonunda oluşan tipik koloniler oluşturan (beyaz, saydam veya hafif mat, mukozlu, yuvarlak, kabarık) 39 izolat seçilerek yatık YMA tüplerine aktarıldı ve buzdolabında +4ºCde muhafaza edildi. Sonraki aşamada bu izolatlar sitolojik ve biyokimyasal analizlerle tanımlanmaya çalışıldı. Bu amaçla izolatlar; Brom Thymol Mavili ve Kongo Kırmızılı YMAda üreme, gram özelliği, hareket, katalaz ve oksidaz testlerine tabi tutularak değerlendirildi. Yapılan sitolojik ve biyokimyasal analizler sonucunda elde edilen 39 izolatın 28inin Rhizobium spp. olduğu tespit edildi.

Isolation of Rhizobium Spp. Bacteria which as Used Microbial Fertilizer from Wild Leguminosarum Plants

In our study used wild leguminous plants (Medicago sativa, M. lupulina and M. varia ) Erzurum belongs to the high altitude (2000 - 2500m ) regions ( Palandöken mountain, Alibaba mountain, Turnagöl mountain, Hasanbaba mountain, Eğerli mountain, Yıldırım mountain, Çubuklu mountain, Deveboynu locality, Kayakyolu locality, Telsizler and Dumlu hill ) were collected during the months of June and July. Nodules were obtained from this plant were sterilized, YMA (Yeast Mannitol Agar) plates were streaked and petri dishes 28+1ºC were incubated for 3-5 days. Colonies appear after incubation typically constitute (white, clear or slightly opaque, mucosity, round, raised) 39 isolates were selected and transferred to tubes and refrigerated YMA were stored at +4ºC. In the next stage, cytological and biochemical analyzes of these isolates were studied to determine. For this purpose isolates; YMA containing bromothymol blue and congo red reproduction, gram stain reaction, movement and subjected to catalase and oxidase tests were evaluated. The cytological and biochemical analysis of results showed that 28 of 39 strains belonged to Rhizobium spp.

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