Uskumru (Scomber scombrus) ve Levrek (Dicentrarchus labrax ) Kemiklerinden Jelatin Ekstraksiyonu ve Karakterizasyonu

Bu çalışmanın amacı uskumru (Scomber scombrus) ve levrek (Dicentrarchus labrax) kemiklerinden jelatin ekstraksiyonu ve fizikokimyasal özelliklerinin belirlenerek ticari balık ve sığır jelatinleri ile karşılaştırılmasıdır. Uskumru ve levrekten elde edilen jelatinlerin verimi sırasıyla %5,98 ile %6,20'dir. Ekstrakte edilen her iki jelatinin ticari jelatinlere göre yüksek protein içeriğine ve düşük nem içeriğine sahip olması önemli düzeyde yüksek saflığa sahip olduğunu göstermektedir. Uskumru ve levrek jelatinlerinin erime sıcaklıkları sırasıyla 25,5 ile 23°C'dir. Uskumru jelatini ticari jelatinlere göre sarımsı ve L* değeri daha yüksek bulunmuştur. Mevcut çalışmada jelatin üretimi için kullanılan uskumru ve levrek kemikleri ticari sığır ve balık jelatini ile karşılaştırıldığında, istenilen fonksiyonel özelliklere sahip ve yüksek verimde jelatin üretimi için ümit verici bir kaynak olarak kullanılabilir.

Extraction and Characterization of Gelatin from Mackerel (Scomber scombrus) and Sea bass (Dicentrarchus labrax) Bones

The aims of this study were to determine the physicochemical properties of extracted gelatins from mackerel (Scomber scombrus) and sea bass (Dicentrarchus labrax) bones and compare with those of commercial fish and bovine gelatins. The yield of gelatin obtained from the bone of mackerel and sea bass were 5.98 and 6.20%, respectively. Two extracted gelatins showed higher protein content, lower moisture content compared to both commercial gelatins, indicates that the gelatin has considerably high purity. Melting temperatures of mackerel and sea bass bone gelatins were 25.5 and 23°C, respectively. Mackerel bone gelatin was yellow in appearance and higher L* value than both commercial gelatins. It can be concluded from the present study that mackerel and sea bass bone are a prospective source to produce gelatin in good yield with desirable functional properties comparable to commercially available mammalian and fish gelatins.

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  • Alfaro AT, Costa CS, Fonseca GG, Prentice C. 2010. Effect of extraction parameters on the properties of gelatin from king weakfish (Macrodon ancylodon) bones. Food Science Technology International. 15: 553-562.
  • Amiza MA, Siti Aishah D. 2011. Effect of drying and freezing of Cobia (Rachycentron canadum) skin on its gelatin properties. International Food Research Journal. 18(1): 159- 166.
  • AOAC. 1999. Official Methods of Analysis. 16th ed. Association of Official Analytical Chemists. Washington, DC.
  • Arnesen JA, Gildberg A. 2006. Extraction of muscle proteins and gelatin from cod head. Process Biochemistry. 41:697- 700.
  • Arnesen JA, Gildberg A. 2007. Extraction and characterization of gelatine from Atlantic salmon Bioresource Technology. 98 (1): 53-57. (Salmo salar) skin.
  • Balti R, Jridi M, Sila A, Souissi N, Nedjar-Arroume N, Guillochon D, Nasri M. 2011. Extraction and functional properties of gelatin from the skin of cuttlefish (Sepia officinalis) using smooth hound crude acid protease-aided process. Food Hydrocolloid. 25: 943-950.
  • Baziwane D, He Q. 2003. Gelatin: The paramount food additive. Food Review International.19: 423-35.
  • Benjakul S, Oungbho K, Visessanguan W, Thiansilakul Y, Roytrakul S. 2009. Characteristics of gelatin from the skins of bigeye snapper, Priacanthus tayenus and Priacanthus macracanthus. Food Chemistry. 116(2): 445-451.
  • Binsi PK, Shamasundar BA, Dileep AO, Badii F, Howell NK. Rheological and functional properties of gelatin from the skin of bigeye snapper (Priacanthus hamrur) fish: influence of gelatin on the gel forming ability of fish mince. Food Hydrocolloid. 23:132-145.
  • Boran G, Mulvaney SJ, Regenstein JM. 2010. Rheological properties of gelatin from silver carp skin compared to commercially available gelatins from different sources. Journal of Food Science. 75: 565-571.
  • BSI (British Standard Institution) 1975. Methods for sampling and testing gelatin (Physical and Chemical Methods), BSI, BS 757, London.
  • Cheow CS, Norizah MS, Kyaw ZY, Howell NK. 2007. Preparation and characterisation of gelatins from the skins of sin croaker (Johnius dussumieri) and short finscad (Decapterus macrosoma). Food Chemistry. 101:386-391.
  • Cho SM, Gu YS, Kim SB. 2005. Extraction optimization and physical properties of yellowfin tuna (Thunnus albacares) skin gelatine compared to mammalian gelatins. Food Hydrocolloid. 19:221-229.
  • Choi S, Regenstein JM. 2000. Physicochemical and sensory characteristics of fish gelatin. Journal of Food Science. 65:194-199.
  • Eastoe JE, Eastoe BA. 1952. Method for the determination of total nitrogen in proteins. The British Gelatin and Glue Research Association Research Report, Series B. 5:1-17.
  • Gelatin Manufactures of Europe. 2008. Official website of GME. Downloaded from http://www. gelatine.org on 8/3/2011.[Erişim Tarihi:8/3/2011] Manufacturer Gelatin Institute of America (GMIA). 2012. Gelatin Hand Book. America.
  • Gildberg A, Arnesen JA, Carlehög M. 2002. Utilisation of cod backbone by biochemical fractionation. Process Biochemistry. 38. 475-480
  • Gómez-Guillén MC, Turnay J, Fernandez-Diaz MD, Ulmo N, Lizarbe MA, Montero P. 2002. Structural and physical properties of gelatin extracted from different marine species.a comparative study. Food Hydrocolloid. 16: 25-34.
  • Gudmundsson M, Hafsteinsson H. 1997. Gelatin from cod skins as affected by chemical treatment. Journal of Food Science. 62(1): 37-39.
  • Hinterwaldner R. 1977. Raw materials. In: (Ward AG. and Courts A) The Science and Technology of Gelatins, New York: Academic Press. pp. 295-314
  • Jamillah B, Harvinder KG. 2002. Properties of gelatins from skins of fish-black tilapia (Oreochromis mossambicus) and red tilapia (Oreochromis nilotica). Food Chemistry. 77: 81- 84.
  • Johnston-Banks FA. 1983. From tannery to table: an account of gelatin Technologies and Chemists. 68: 141-145.
  • Johnston-Banks FA., 1990. Gelatin. In: (Harris P) Food gels. London, Elsevier Appl. Sci, pp. 233-289.
  • Jongjareonrak A, Benjakul S, Visessanguan W, Prodpran T, Tanaka M. 2006. Characterization of edible films from skin gelatin of Brown stripe red snapper and bigeye snapper. Food Hydrocolloid. 20: 492-501.
  • Jongjareonrak A, Rawdkuen S, Chaijan M., Benjakul S, Osako K, Tanaka, M. 2010. Chemical compositions and characterisation of skin gelatin from farmed giant (Pangasianodon Technology. 43: 161-165. catfish and gigas). LWT- Food Science
  • Karim AA, Bhat, R. 2009. Fish gelatin: properties, challenges, and prospecte as an alternative to mammalian gelatins. Food Hydrocolloid. 23 (3): 563- 576.
  • Kasankala LM, Xue Y, Weilong Y, Hong SD, He Q. 2007. Optimization of gelatin extraction from grass carp (Catenopharyngodon idella) fish skin by response surface methodology. Bioresource Technology. 98(17): 3338-3343.
  • Khiari Z, Rico D, Martin-Diana AB, Barry-Ryan C. 2011. The extraction of 535 gelatin from mackerel (Scomber scombrus) heads with the use of different organic 536 acids. Journal of Fisheries Science. 5: 52-63.
  • Liu HY, Han J, Guo SD. 2009. Characteristics of the gelatin extracted from channel catfish (Ictalurus punctatus) head bones. LWT - Food Science and Technology.42(2): 540- 544.
  • Mohtar NF, Perera CO, Quek, SY. 2010.Optimisation of gelatine extraction from hoki (Macruronus novaezelandiae) skins and measurement of gel strength and SDS-Page. Food Chemistry. 122: 307-313.
  • Mohtar NF, Perera CO, Quek SY. 2011. Utilisation of gelatine from NZ hoki (Macruronus novaezelandiae) fish skins. International Food Resource Journal. 18(3): 1111-1115.
  • Montero P, Gómez-Guillén MC. 2000. Extracting conditions for megrim (Lepidorhombus boscii) functional properties of the resulting gelatin. Journal of Food Science. 65(3):434-438. skin collagen affect
  • Montero P, Gómez-Guillén MC, Borderías AJ. 1999. Functional characterization of muscle and skin collagenous material from hake (Merluccius merluccius L.) Food Chemistry. 65: 55-59.
  • Montero P, Fernandez-Dlaz MD, Gomez-Guillen, MC. 2002. Characterization of gelatin gels by high pressure. Food Hydrocolloid. 16: 197-205.
  • Muyonga JH, Cole CGB, Duodu KG. 2004. Extraction and physico-chemical characterisation of Nile perch (Lates niloticus) skin and bone gelatin. Food Hydrocolloid. 18:581-592.
  • Nikoo M, Xu X, Benjakul S, Wu G, Ramirez-Suarez JC, Ehsani A, Kasankala LM, Duan X, Abbas S. 2011. Characterization of gelatin from the skin of farmed Amur sturgeon Acipenser schrenckii. International Aquatic Research. 3: 135-145.
  • Ninan G, Jose J, Abubacker Z. 2011.Preparation and characterization of gelatin extracted from the skins of Rohu (LabeoRohıta) and Common Carp (CyprınusCarpıo). J. Food Processing and Preservation. 35:143-162.
  • Norziah MH, Al-Hassan A, Khairulnizam AB, Mordi MN, Norita M. 2009. Characterization of fish gelatin from surimi processing wastes: Thermal analysis and effect of transglutaminase on gel properties. Food Hydrocolloid. 23: 1610-1616.
  • Ockerman HW, Hansen C. L.1988.Animal by-product processing. England: Ellis Horwood Ltd.
  • Pranoto Y, Lee CM Park HJ. 2007. Characterizations of fish gelatin films added with gellan and k-carrageenan. Food Science and Technology. 40(5): 766-774.
  • Ratnasari I, Yuwono SS, Nusyam H, Widjanarko SB. 2013. Extraction and characterization of gelatin from different fresh water fishes as alternative sources of gelatin. International Food Resource Journal. 20(6): 3085-3091.
  • Songchotikunpan P, Tattiyakul J, Supaphol P. 2008. Extraction and electrospinning of gelatin from fish skin. International Journal of Biological Macromolecules. 42: 247-255.
  • Steel RGD, Torrie JH. 1980. Principles and procedures of statistics: A biometrical approach 2nd ed. New York, McGraw-Hill.
  • Taheri A, Abedian Kenari, AM, Gildberg A, Behnam S. 2009. Extraction and physicochemical characterization of greater lizardfish (Saurida tumbil) skin and bone gelatin. Journal of Food Science. 74: 160-165.
  • Tavakolipour H. 2011. Extraction and evaluation of gelatin from silver carp waste. World Journal of Fish and Marine Sciences . 3(1): 10-15.
  • Techochatchawal K, Therdthai N, Khotavivattana S. 2009. Development of calcium supplement from the bone of Nile tilapia (Tilapia nilotica). Asian Journal of Food and Agro- Industry. 2: 539-546.
  • Wainewright FW. 1977. Physical tests for gelatin and gel products. In: (Ward, AG and Courts A), The science and technology of gelatins. London, Academic Press Inc. p. 507-531.
  • Wangtueai S, Noomhorm A. 2009. Processing optimization and characterization of gelatin from lizardfish (Saurida spp.) scales. LWT - Food Science and Technology. 42: 825-834.
  • Ward AG, Courts A. 1977. The Science and Technology of Gelatins. Academic Press. New York.
  • Yang H, Wang Y, Jiang M, Oh JH, Herring J, Zhou P. 2007. 2- step optimization of the extraction and subsequent physical properties of Channel Catfish (Ictalurus punctatus) skin gelatin. Journal of Food Science. 72(4):188-195.
  • Zhou P, Regenstein JM. 2004. Optimization of extractionconditi ons for pollock skin gelatin. Journal of Food Science. 69: 393-398.
  • Zhou P, Regenstein JM. 2005. Effects of alkaline and acid pretreatments on Alaska Pollock skin gelatin extraction. Journal of Food Science. 70(6): 392 396.