Yumurtacı Tavuklarda Biberiye (Rosmarinus officinalis L.) Uçucu Yağının Bazı Kan Parametreleri ve İnce Bağırsak Mikroflorası Üzerine Etkileri

Bu çalışma, yumurtacı tavuklarda biberiye (Rosmarinus officinalis L.) uçucu yağının bazı kan parametreleri ve jejunum bakteri içeriği üzerindeki etkilerini, antibiyotik (klortetrasiklin) ve E vitamini (?-tokoferol asetat) ilaveli yem grupları ile karşılaştırmalı olarak belirlemek üzere yapılmıştır. Çalışmada 32 haftalık yaşta 240 adet Bovans genotipi beyaz yumurtacı tavuk (5 tekrarlamalı ve her bir tekerrür grubunda 8 tavuk olmak üzere), 6 ana gruba ayrılmıştır. Gruplardan biri standart bazal yemle beslenirken, diğerleri aynı yeme 500 mg/kg antibiyotik, 200 mg/kg E vitamini, 100, 200 ve 300 mg/kg biberiye uçucu yağı içeren karma yemlerle 90 gün süreyle beslenmişlerdir. Karma yeme ilave edilen katkıların incelenen kan parametreleri üzerine herhangi bir etkisi saptanmamıştır. Ancak antibiyotik ve biberiye uçucu yağının tüm dozları bağırsak jejunum bakteri içeriğini etkilemiş ve muhtemel koliform, fekal koliform, Escherichia coli (E. coli) ve Clostridium perfringens (Cl. perfringens) bakterilerini önemli düzeyde düşürmüştür. Biberiyenin 100 mg/kg uçucu yağ dozu, incelenen tüm bakteri türlerinde, antibiyotik ile aynı etkiye sahip olurken, 300 mg/kg doz uygulamaları muhtemel fekal koliform 200 ve 300 mg/kg dozların ise E. coli üzerinde antibiyotikten çok daha etkili olmuştur. Sonuç olarak biberiye uçucu yağının yüksek antimikrobiyal etkiye sahip olduğu ve bazı patojen bakterilere karşı koruyucu olarak yumurtacı tavuk karma yemlerinde kullanılabileceği söylenebilir.

Influences of Rosemary Essential Oil on Some Blood Parameters and Small Instentine Microflora Laying Hens

This experiment was designed to investigate the effects of antibiotic, vitamin E and rosemary essential oils in various doses on some blood parameters, beneficial and harmful microorganisms in instentine jejunum microflora of lying hens. The experiment was conducted according to random parcel experiment design. A total of 240 Bovans genotype and 32 weeks aged were equally divided into 6 groups (8 hens in each group with 5 replicates). While the control group was fed with basal diet the treatment groups were supplemented with 500 mg/kg antibiotics; 200 mg/kg vitamin E; 100, 200, and 300 mg/kg REO to basal diet for 90 consecutive days. The result showed that there were no effects of all additions to basal diets on examined blood parameters. Antibiotic and all rosemary essential oils doses to basal diet affected the instentine jejunum bacteria content by decreasing probable coliforms, faecal coliforms, Escherichia coli (E. coli) and Clostridium perfringens (Cl. perfringens) bacteria level significantly. While a 100 mg/kg rosemary essential oils dose was of the same influence with antibiotic (500 mg/kg) on all bacteria species, 200 and 300 mg/kg doses applications were more effective on E. coli compare to antibiotics. 300 mg/kg doses applications dose was more effective on probable faecal coliforms than antibiotics. As conclusion, the results shows the rosemary essential oils has the high antimicrobial effect on some pathogenic bacterias and could be used in laying hen for feed addition.

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Abd El-Hack ME, Mahgoub SA, Alagawany M, Dhama K. 2015. Influences of dietary supplementation of antimicrobial cold pressed oils mixture on growth performance and intestinal International Journal of Pharmacology, 11(7): 689-696.
Al-Kassie GAM, Mohammed MF, Hamood MF, Jameel YJ. 2008. The effect of anise and rosemary on the microbial balance in gastro ıntestinal tract for broiler chicks. International Journal of Poultry Science, 7(6): 610-612.
Altıntaş A, Fidancı UR. 1993. Evcil hayvanlarda ve insanda kanın biyokimyasal normal değerleri. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 40(2): 173-186.
Anonim 2005. Merck Gıda Mikrobiyolojisi Uygulamaları. Ed: Halkman AK, Başak Matbaacılık ve Tanıtım Hizmetleri Ltd. Şti., Ankara. 358 sayfa. ISBN: 975-00373-0-8.
AOAC 1984. Official Methods of Analysis (14th ed.), Association of Official Analytical Chemists, Washington, DC.
Belenli D, Udum D, Cengiz SŞ, Polat Ü. 2015. Influence of various volatile oils as a dietary supplement on biochemical and performance parameters in broilers. Journal of Environmental Sciences, 9(25): 47-55.
Bendich A. 1998. Vitamin E and immune functions. Basic Life Science. 49: 615-620.
Beyazıtoğlu Ş. 2009. Yüksek sıcaklık altında rasyona biyoantioksidan (alfa-tokoferol asetat, karvakrol, karnosik asit) katkısının yumurtacı tavuklarda performans, yumurta verimi, yumurta kalitesi ve immun sistem üzerine etkileri. Yüksek Lisans Tezi (Basılmamış). Çukurova Üniversitesi Fen Bilimleri Enstitüsü, 72 s Adana.
Bölükbaşı SC, Erhan MK, Kaynar Ö. 2008. The effect of feeding thyme, sage, and rosemary oil on laying hen performance, cholesterol and some protein ratio of egg yolk and Eschrichia Coli count in feces. Arciv. Für Geflugelkunde, 72: 231-237.
Buğdaycı KE, Ergün A. 2011. Esansiyel yağ ve/veya probiyotiğin broylerlerde performans, immun sistem ve bazı kan parametreleri üzerine etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 58, 279-284.
Carpenter KJ, Clegg KM. 1956. The metabolizable energy of poultry feeding stuffs in relation to their chemical composition. Journal Science Food Agriculture, 7(1):45-51.
Case GL, He L, Mo H, Elson CE. 1995. Induction of geranyl pyrophosphate pyrophosphatase activity by cholesterol- suppressive isoprenoids, Communication Lipids, 30(4): 357- 359.
Cervantes H. 2006. Banning antibiotic growth promoters: Learning International, 45:14-15. experience. Poultry
Ceylan A. 1997. Tıbbi Bitkiler-II, Ege Üniversitesi Ziraat Fakültesi Yayını, No:481, ISBN:975-483-362-1, Bornova- İzmir.
Ceylan N, Çiftçi İ, İlhan Z. 2003. Büyütme faktörü antibiyotiklere alternatif yem katkılarının etlik piliçlerde besi performansı ve bağırsak mikroflorası üzerine etkileri. Turkish Journal of Veterinary and Animal Sciences, 27: 727-733.
Christaki E, Bonos E, Giannenas I, Florou-Paneri P. 2012. Evaluation of oregano and ?-tocopheryl acetate on laying japanese quail diets. Journal of Basic and Applied Sciences, 8: 238-242.
Çiftçi M, Şimşek ÜG, Azman MA, Çerçi İH, Tonbak F. 2013. The effects of dietary rosemary (Rosmarinus officinalis L.) oil supplementation on performance, carcass traits and some blood parameters of japanese quail under heat stressed condition. Kafkas Universitesi Veteriner Fakültesi Dergisi, 19(4): 595-599.
Crespy V, Williamson G. 2004. International Research Council on Food, Nutrition, and Cancer, A review of the health effects of the catechins in in vivo animal models, The Journal of Nutrition, 134: 3431-3440.
Crowell PL. 1999. Prevention and therapy of cancer by dietary monoterpenes. Journal of Nutrition, 129(3): 775S-778S.
Düzgüneş O, Kesici T, Kavuncu O, Gürbüz F. 1987. Araştırma ve deneme metodları (İstatistik metodları-II). 186 s, Ankara.
Gachkar L, Yadegari D, Rezaei MB, Taghizadeh M, Astaneh SA, Rasooli I. 2007. Chemical and biological characteristics of cuminum cyminum and Rosmarinus officinalis essential oils. Food Chemistry, 102:898-904.
Ghazalah AA, Ali AM. 2008. Rosemary leaves as a dietary supplement for growth in broiler chickens. International Journal of Poultry Science, 7(3): 234-239.
Günal M, Yaylı G, Kaya N, Karahan N, Sulak O. 2006. The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, ıntestinal microflora and tissue of broylers. Int. Journal of Poultry Science, 5(2): 149-155.
Govaris A, Florou-Paneri P, Botsoglou E, Giannenas I, Amvrosiadis I, Botsoglou N. 2007. The inhibitory potential of feed supplementation with rosemary and/or ?-tocopheryl acetate on microbial growth and lipid oxidation of turkey breast during refrigerated storage. Lwt-Food Science and Technology, Phytochemistry, 68(6): 840-852.
Iqbal R, Aziz T, Sarfaraz I, Shabir R, Ansari MS, Malik MF, Saleem R, Zahra A, Mehwish S. 2013. Effect of vitamin E and selenium on ımmunity, egg production and lever function in laying hens (HY-LINE W-98). Middle-East Journal of Scientific Research, 14(9): 1165-1170.
Kaya A, Kaya H, Macit M, Çelebi Ş, Esenbuğa N, Yörük MA, Karaoğlu M. 2013. Effects of dietary ınclusion of plant extract mixture and copper into layer diets on egg yield and quality, yolk cholesterol and fatty acid composition. Kafkas Universitesi Veteriner Fakültesi Dergisi, 19(4): 673-679.
Lee KW, Everts H, Beynen AC. 2004. Essential oils in broiler nutrition. Int. Journal of Poultry Science, 3(12): 738-752.
Leshchinsky TV, Klasing KC. 2001. Relationship between the level of dietary vitamin E and the immune response of broiler chickens. Poultry Science, 80: 1590-1599.
Macala LJ, Yu RK, Ando S. 1983. Analysis of brain lipids by high densitometry. The Journal of Lipid Research, 24(9): 1243- 1250. chromatography and
Manafi M, Hedayati M, Yari M. 2014. Application of rosemary (Rosmarinus officinalis L.) essence on chicks fed aflatoxin B1: Impacts on internal organ weights, biochemical traits and mortality. Research in Zoolog, 4(1): 13-19.
Mathlouthi N, Bouzaienne T, Oueslati I, Recoquillay F, Hamdi M, Urdaci M, Bergaoui R. 2012. Use of rosemary, oregano, and a commercial blend of essential oils in broiler chickens: In vitro antimicrobial activities and effects on growth performance. Journal Animal Science, 90(3): 813-823.
Nockels CF, Slota SJ, Mathians MM. 1992. Effects of dietary vitamin E level and unsaturation of fatty acids on chick immune responses. J. Nut. Immun, 1(4): 83-100.
O'Gara E, Hill DJ, Maslin DJ. 2000. Activities of garlic oil, garlic powder, and their diallyl constituents against Helicobacter pylori. Appl. Environ. Microbiol., 66(5): 2269- 2273.
Polat U, Yesilbag D, Eren M. 2011. Serum biochemical profile of broiler chickens fed diets containing rosemary and rosemary volatile oil. J. Biol. Environ. Science, 5(13), 23- 30.
Qureshi AA, Lehmann JW, Peterson DM. 1996. Amaranth and its oil inhibit cholesterol biosynthesis antimicrobial properties of plant essential oils and in 6-week-old female chickens. Journal of Nutrition, 126: 1972-1978.
Radwan Nadia L, Hassan RA, Qota EM, Fayek HM. 2008. Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. International Journal of Poultry Science, 7(2): 134- 150.
Rice D, Kennedy K. 1988. Vitamin E function and effects of deficiency. British Veterinary Journal, 144(5):482- 496.
Santonyo S, Cavero S, Janıme L, Ibenaz E, Senorans FJ, Reglero G. 2005. Activity of Rosmerinus officinalis L. esaaential oil obtained via supercritical fluid extraction. J. Food Protect., 68(4): 790-795.
Şimşek ÜG, Güler T, Çiftçi M, Ertaş ON, Dalkılıç B. 2005. Esans yağ karışımının (kekik, karanfil ve anason) broylerlerde canlı ağırlık, karkas ve etlerin duyusal özellikleri üzerine etkisi. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 16(2):1-5.
Swain BK, Johri TS, Majumdar S. 2000. Effect of supplementation of vitamin E, selenium and their different combinations on the performance and immune response of broilers. British Poultry Science, 41(3): 287-292.
Tanaka J, Fujiwara H, Torisu M. 1979. Enhancement of Hepler T cell activity by dietary supplementation of vitamin E in mice. Immunology, 38(4): 727-734.
Turkmen G, Öztabak K, Demircan H, Toker N, Matur E. 2006. The effect of vitamin E on cellular immune responses in laying hens forced-moulted by different methods. European Poultry Sci. Arch.Geflügelk., 70( 1): 28-34. 2006, ISSN 0003-9098.
Wenk C. 2000. Why All The Discussion About Herbs? Biotechnolgy in the feed industry. Proc. of Alltech's 16 Th Annu. Symp. Alltech Technical Puplications, Nottingham Universty Press, Nicholasville, KY. Pages:79-96.
Yıldız CH. 2007. Carvacrol, Tymol ve Rosmarinic Asit İçeren Bitki Ekstraktlarının Etlik Piliçlerde Performans, Sindirim Kanalı Histomorfolojisi ve Kan Parametreleri Üzerine Etkileri (doktora tezi, basılmamış). TÜ, Fen Bilimleri Enstitüsü, Tekirdağ.
Zaoui A, Cherrah Y, Alaoui K, Mahassine N, Amarouch H, Hasar M. 2002. The effects of nigella sativa fixed oil on blood homeostatis in rat. Journal of Ethnopharmacology, 79(1): 23-26.