Systematic Determination of The Ultrastructure of Local Faba Bean (Vicia faba L.) Seeds Using Light and Scanning Electron Microscopes

The present study was conducted to determine the seed morphologies and macromorphological characteristics of fifteen local faba bean (Vicia faba L.) genotypes collected from different region of Northern Cyprus. The characteristics in the seeds were evaluated using a light microscope (LM) and scanning electron microscope (SEM). SEM pictures were taken of the whole seed and its details. The scope of the research was examined comparatively using LM and SEM, and the seed shapes, surface ornamentation, and quantitative measurements were determined. Different characteristics for seed size and surface were found among the local faba beans accessions. Seed dimensions were between 12.44 and 24.26 mm long and between 9.49 and 17.45 mm wide, colors ranged from yellowish-brown to dark brown, and ornamentation of the seed coat varied. The local faba bean seeds were of subprolate and prolate types. We believe that using this technique to determine the differences among the seeds of different genotypes may play a role in helping to create different programs that can choose specific genotypes to improve cultivars based on the seed characteristics.

___

Al Barri T, Shtaya MJY. 2013. Phenotypic characterization of faba bean (Vicia faba L.) landraces grown in Palestine. Journal of Agriculture Science, 5(2): 110-117. doi: https:// doi.org/10.5539/jas.v5n2p110

Ammar MH, Alghamdi SS, Migdadi HM, Khan MA, Ehab HEH, Sulieman AA. 2015. Assessment of genetic diversity among faba bean genotypes using agro-morphological and molecular markers. Saudi Journal of Biological Sciences 22: 340-350. doi: https://doi.org/10.1016/j.sjbs.2015.02.005

Balkaya A, Özbakır M, Karaağaç O. 2010. Evaluation of variation and fruit characterization of pumpkin (Cucurbita moschata duch.) populations collected from black sea region. The Journal of Agricultural Science, 16(1): 17-25.

De Cillis F, Leoni B, Massaro M, Renna M, Santamaria P. 2019. Yield and quality of faba bean (Vicia faba L. var. major) genotypes as a vegetable for fresh consumption: a comparison between Italian landraces and commercial varieties. Agriculture. 9: 253. https://doi.org/10.3390/agriculture9120 253.

Duc G, Bao S, Baum M, Redden R, Sadiki M, Suso MJ, Vishniakova M, Zong X. 2010 Diversity maintenance and use of Vicia faba L. genetic resources. Field Crops Research, 115: 270–278.

Ekincialp A, Şensoy S. 2013. Determination of some vegetable features of Van lake basin bean genotypes. YYU, Journal of Agricultural Sciences, 23(2): 102-111. (in Turkish)

Fagundez J, Izco J. 2011. Seed morphology and anatomy of the Mediterranean pentamerous species of Erica (Ericaceae). Turkish Journal of Botany, 35: 643–651.

Gasim S, Abel S, Link W. 2004. Extent, variation and breeding impact of natural cross-fertilization in German winter faba beans using hilum colour as marker. Euphytica, 136: 193- 200.

Gnanasambandam A, Paull J, Torres A, Kaur S, Leonforte T, Li H, Zong X, Yang T, Materne M. 2012. Impact of molecular technologies on faba bean (Vicia faba L.) breeding strategies. Agronomy, 2: 132-166, doi:https://doi.org/10.3390/ agronomy2030132

Göl Ş, Doğanlar S, Frary A. 2016. Genetic diversity and population structure of faba bean (Vicia faba) from Turkey. HortScience, 51(8): 980–983. doi: https://doi.org/10.21273/ HORTSCI11022-16

Javadi F, Yamaguchi H. 2004. A note on seed coat and plumule morphological variation in the genus Cicer (Fabaceae). Biology, 56: 7–16.

Juan R, Pastor J, Fernandez I. 1994. Seed morphology in Veronica L. (Scrophulariaceae) from south-west Sapin. Botanical Journal of the Linnean Society, 115: 133–143.

Kahraman A, Chidire H, Dogan M. 2014. Anatomy, macro- and micromorphology of Lathyrus sect. Nissolia (Fabaceae) and their taxonomic significance. Proceedings of the National Academy of Sciences, 84: 407–417.

Karaköy T, Demirbaş A, Toklu F, Özkan H, Karagöl ET, Uncuer D, Gürsoy N. 2017. Agronomic and morphological characterization of faba bean landraces (Vicia faba l.) collected from different region of Turkey. KSU Journal of Natural. Science, 20 (Special issue): 356-361. doi: https://doi.org/10.18016/ksudobil.349299.

Kaya A, Ünal M, Özgökçe F, Doğan B, Martin E. 2011. Fruit and seed morphology of six species previously placed in Malcolmia (Brassicaceae) in Turkey and their taxonomic value. Turkish Journal of Botany, 35: 653-662.

Kobal Bekar N, Sağlam N, Balkaya A. 2019. The Evaluation of Variation in terms of Broad Bean Characteristics and Broad Bean Quality of Some Bean Genotypes. Yuzuncu Yil Univ. Journal of Agriculture Science, 29: 127-135.

Kurkina YN, Kieu NTZ, Kolesnikov DA, Maradudina ON, Lazarev AV. 2020. Analysis of the ultrastructure of pollen grains and seeds to identify plant materials. EurAsian Journal of BioSciences 14: 1489-1494.

Madakbaş SY, Özçelik H, Ergin M. 2006. Determination of the differences between the lines determined from the dwarf green bean populations in the Çarşamba Plain. Harran Univ. Journal of Agriculture Faculty, 10: 71-77.

Manga İ, Acar Z, Ayan İ. 1995. Legume forage crops. OMU Tech., 274: 65.

Mirzaei L, Assadi M, Nejadsatar T, Mehregan I. 2015. Comparative seed and leaf micromorphology of Colutea species (Fabaceae) from Iran. Environmental and Experimental Biology. 13: 183–187.

Mosquero MAM, Juan R, Pastor JE. 2002. Morphological and anatomical studies on nutlets of Nepeta L. (Lamiaceae) from South-West Spain. Acta Botanica Malacitana, 27: 15-26

Özbek F, Ekici M. 2014. Morphological, anatomical, pollen and seed morphological properties of Melilotus bicolor Boiss. & Balansa (Fabaceae) endemic to Turkey. Australian Journal of Crop Science, 8: 543-549.

Riahi M, Zarre S. 2009. Seed development in Astragalus cemerinus and A. ruscifolius (Fabaceae), and its systematic implications. Acta Biologica Cracoviensia Series Botanica, 51: 111–117.

Sarı N, Solmaz İ, Pamuk S, Çetin MB. 2016. Seed and pod characteristics of different seed coat color beans collected from Black Sea Region. Academia Journal of Agricultural Research, 5: 21-28. (in Turkish).

Sözen Ö, Karadavut U. 2016. Determination of morphological and phenological properties of faba beans grown in Eastern Mediterranean Region of Turkey. Journal of Field Crops Central Research Institute, 25: 209-217. doi: https://doi.org/10.21566/tarbitderg.281344

Toker MC. 2004. Plant Morphology. Ank. Univ. Faculty of Science. (second edition in Turkish), 56p.

Torres AM, Roman B, Avila CM, Satovic Z, Rubiales D, Sillero JC, Cubero JI, Moreno MT. 2006. Faba bean breeding for resistance against biotic stresses: towards application of marker technology. Euphytica. 147: 67-80.

Yılmaz N. 2020. Morphological and molecular characterization of local faba bean (Vicia faba l.) accessions using inter-simple sequence repeat (ISSR) markers. Fresenius Environmental Bulletin 29: 3756-3763.
Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: 12
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)
Sayıdaki Diğer Makaleler

Baklagillerde (Vicia faba L.) Çiçekler ve Bakla Fasulyesi Çiçek Çayında L-DOPA (L-3, 4-Dihidroksifenilalanin) İçeriğinin Belirlenmesi

Hatice BOZOĞLU, Merve BEZMEN

Farmers’ Pest Management Practices of Stored Faba Bean and their Implication to Food Security in Farta District, North West Ethiopia

Wondale ENDSHAW, Berhanu HİRUY

Root and Shoot Traits of Spring Wheat Cultivars with Wild-Type and SemiDwarf Rht Alleles at Early and Late Growth Stages

HAYATİ AKMAN, Philip BRUCKNER

Effects of Allegations Regarding the Use of Antibiotics and Hormones in Diets on Consumer Perceptions, Attitudes and Behaviors towards Broiler Meat Consumption

Kerem KARASU, ERGİN ÖZTÜRK

Effect of Different Inorganic Substrates on Growth Performance of African Catfish (Clarias gariepinus, Burchell 1822) and Lettuce (Lactuca sativa L.)

Murat YEŞİLTAŞ, Mehmet Ali Turan KOÇER, Hüseyin SEVGİLİ, Edis KORU

Effect of Natural Seed Aging on Root and Shoot Traits in Bread Wheat (Triticum aestivum L.) Cultivars

HAYATİ AKMAN

Antioxidant and Cytotoxic Potential of Local Endemic Plant Pastinaca zozimoides Fenzl

Cihan DÜŞGÜN, Teoman KANKILIC, CEMİL İŞLEK, Dilara Fatma BALI, Özgür KANKILIÇ

Systematic Determination of The Ultrastructure of Local Faba Bean (Vicia faba L.) Seeds Using Light and Scanning Electron Microscopes

NİHAT YILMAZ, Handan ŞAPÇI SELAMOĞLU, Zeynep ARI, Hatice BEKCİ

Evaluation of Stabilized Soymilk Prepared from Sprouted Soybean with Orange Flesh Sweet Potato Starch for Ambient Storage

Innocent Nwazulu OKWUNODULU, Anselm Uzochukwu ONWUZURİKE, Alexzandria Obianuju OCHİOG, Comfort Ugochi UZOCHUKWU

Different sources of phosphorus fertilizers and soil amendments affected the phosphorus and cadmium content in soil, roots and seeds of maize (Zea mays L.)

P. C. U. WANNİNAYAKE, M. A. P. W. K. MALAVİARACHCHİ, R. P. HETTİARACHCHİ, P. N. YAPA