Silimarin’in Paklitaksel Kaynaklı Nefrotoksisiteye Karşı Koruyucu Etkileri

Kanser tedavisinde kullanılan ilaçların etkileri yalnızca kanser hücrelerine özgü olmayıp sağlıklı hücreleri de olumsuz etkilemektedir. Bu çalışmada antikanser bir ilaç olan paklitaksel’in (PAX) böbrek dokusundaki olası zararlı etkilerine karşı antioksidan etkileriyle öne çıkan silimarin’in (SLY) olası koruyucu etkilerinin ortaya konulması amaçlandı. Toplam 28 adet Sprague-Dawley cinsi dişi sıçandan rastgele bir seçimle: Kontrol, PAX, SLY ve PAX + SLY olmak üzere dört grup (n=7) oluşturuldu. PAX grubuna 2 mg/kg intraperitoneal yolla PAX, SLY grubuna oral gavaj sonda ile 100 mg/kg SLY, PAX + SLY grubuna ise önceki gruplarla aynı doz ve yolla PAX ve SLY uygulandı. Deneysel işlemlerin sonunda hayvanlardan alınan kan ve böbrek dokularında yapılan biyokimyasal analizlerde PAX’ın, böbrek dokusunda oksidatif stresi, serumda ise kreatinin (Cr) ve kan üre azotu (blood urea nitrogen, BUN) seviyelerini artırdığı tespit edildi. Böbrek dokusunda yapılan histopatolojik incelemelerde PAX’ın renal korpüskül atrofisi, fırçamsı kenarda hasar, vakuolar dejenerasyon ve deskuamasyon gibi patolojik değişikliklere yol açtığı gözlendi. Antienflamatuar, antiapoptotik ve antioksidan etkilere sahip bir farmakolojik ajan olan SLY uygulanması ise, PAX kullanımı sonucu meydana bu gelen patolojik değişiklikleri büyük oranda engelledi. Mevcut çalışmada elde edilen sonuçlar PAX ile yapılacak tedavilerde böbrek dokusunda şekillenecek zararlı etkilere karşı koruyucu olarak SLY’nin kullanılabileceğini gösterdi.

Silymarin Protects Kidneys from Paclitaxel-Induced Nephrotoxicity

The effects of drugs used in cancer treatment are not only specific to cancer cells but also negatively affect healthy cells. This study, it was aimed to investigate the protective effects of silymarin (SLY), which stands out with its antioxidant effects, against the possible harmful effects of paclitaxel (PAX), an anticancer drug, on kidney tissue. A total of 28 Sprague-Dawley female rats were randomly selected into four groups (n=7): Control, PAX, SLY, and PAX + SLY. PAX was administered 2 mg/kg to the PAX group by intraperitoneally (i.p.); SLY was administered 100 mg/kg to the SLY group by oral gavage. Also, The PAX + SLY group was administered the same dose and route of PAX and SLY as the previous groups. In the biochemical analyzes performed in blood and kidney tissues taken from animals at the end of the experimental procedures, it was determined that PAX increased oxidative stress in kidney tissue and creatinine (Cr) and blood urea nitrogen (BUN) levels in serum. In the histopathological examinations of the kidney tissue, it was observed that PAX caused pathological changes such as renal corpuscle atrophy, damage to the brush border, vacuolar degeneration, and desquamation. The administration of SLY, a pharmacological agent with antiinflammatory, anti-apoptotic, and antioxidant effects, largely prevented these pathological changes that occurred as a result of PAX use. The results obtained in the current study showed that SLY can be used as a protective agent against harmful effects on kidney tissue in treatments with PAX.

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