Mehdi GHORBANİ, Aghil GHORBANİ, Mansoor OMIDI, Seyed Mohammad HASHEMİ

Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

Common purslane (Portulaca oleracea L.) is an annual plant as one of the natural sources for noradrenaline hormone. In this research, hairy root culture of purslane was established by using Agrobacterium rhizogenes strain ATCC 15834. In the following, Box-Behnken model of response surface methodology (RSM) was employed to optimize B5 medium for the growth of P. oleracea L. hairy root line. According to the results, modelling and optimization conditions, including sucrose, CaCl2 .H2O, H2PO4 and NO3 - /NH4 + concentrations on maximum dry weight (0.155 g) and noradrenaline content (0.36 mg.g-1 DW) was predicted. These optimal conditions predicted by RSM were confirmed the enhancement of noradrenaline production as an application potential for production by hairy root cultures.

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Ahmadi Moghadam Y, Piri K, Bahramnejad B, Ghiyasvand T. 2014. Dopamine production in hairy root cultures of Portulaca oleracea (Purslane) using Agrobacterium rhizogenes. Journal of Agricultural Science and Technology 16: 409-420.

Ahmadi Moghadam Y, Piri K, Bahramnejad B, Habibi P. 2011. Hairy roots induction in purslane (Portulaca oleracea Linn.) using Agrobacterium rhizogenes. Planta Medica 77: PB24.

Amirul Alam M, Juraimi A, Rafii MY, Hamid A, Kamal Uddin M, Alam MZ, Latif MA. 2014. Genetic improvement of Purslane (Portulaca oleracea L.) and its future prospects. Molecular Biology Reports 41: 7395-7411.

Bas D, Boyacı IH. 2007. Modeling and optimization II: Comparison of estimation capabilities of response surface methodology with artificial neural networks in a biochemical reaction. Journal of Food Engineering 78: 846-854.

Chen J, Shi YP, Liu JY. 2003. Determination of noradrenaline and dopamine in Chinese herbal extracts from Portulaca oleracea L. by high-performance liquid chromatography. Journal of Chromatography A 1003: 127-132.

Gamborg OL, Miller RA, Ojima K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50: 151-158.

Mairet F, Sierra J, Glorian V, Villon P, Shakourzadeh K, BoitelConti M. 2009. A new approach to define optimized range of medium composition for enhancement of hairy root production in fed-batch process. Bioprocess and Biosystems Engineering 32: 257-265.

Maschke RW, Geipel K, Bley T. 2015. Modeling of plant in vitro cultures: Overview and estimation of biotechnological processes. Biotechnology and Bioengineering 112: 1-12.

Pirian K, Piri K. 2013. Influence of yeast extract as a biotic elicitor on noradrenaline production in hairy root culture of Portulaca oleracea L. International Journal of Agronomy and Plant Production 4: 2960-2964.

Pirian K, Piri K, Hamedan I. 2012. Effect of methyl jasmonate and salicylic acid on noradrenaline accumulation in hairy roots of Portulaca oleracea L. International Research Journal of Applied and Basic Sciences 3: 213-218.

Safdari Y, Kazemitabar S. 2009. Plant tissue culture study on two different races of purslane (Portulaca oleracea L.). African Journal of Biotechnology 8: 5906-5912.

Safdari Y, Kazemitabar SK. 2010. Direct shoot regeneration, callus induction and plant regeneration from callus tissue in mose rose (Portulaca grandiflora L.). Plant Omics 3: 47-51.

Shen H, Tang G, Zeng G, Yang Y, Cai X, Li D, Zhou N. 2013. Purification and characterization of an antitumor polysaccharide from Portulaca oleracea L. Carbohydrate Polymers 93:395- 400.

Srivastava S, Srivastava AK. 2012. Azadirachtin production by hairy root cultivation of Azadirachta indica in a modified stirred tank reactor. Bioprocess and Biosystems Engineering 35: 1549- 1553.

Sung LS, Huang SY. 2000. Medium optimization of transformed root cultures of Stizolobium hassjoo producing L−DOPA with response surface methodology. Biotechnology Progress 16: 1135-1140.

Tian JL, Liang X, Gao PY, Li DQ, Sun Q, Li LZ., Song SJ. 2013. Two new alkaloids from Portulaca oleracea and their cytotoxic activities. Journal of Asian Natural Products Research 16: 259- 264.

Zargari A. 1991. Medicinal Plants. University of Tehran Press.