Probiyotik Lactobacillus Süpernatantlarının Streptococcus Mutans'a Karşı İnhibitör Etkisi ve Biyofilm Oluşumunu Önleme

Probiyotik mikroorganizmaların ürettiği biyoaktif maddeler, Streptococcus mutans gibi patojenik mikroorganizmaların büyümesini ve biyofilm oluşumunu engellemeye yardımcı olabilirler. Diş çürükleri, esas olarak S. mutans'ın neden olduğu bakteriyel biyofilm oluşumuyla başlayan, çok faktörlü kronik bir enfeksiyon hastalığıdır. Bu çalışmada oral probibiyotik olarak Lactobacillus spp. suşlarının karakteristik özellikleri araştırılmıştır. On iki Lactobacillus spp. türü fermente süt ve süt ürünlerinden elde edilmiştir. S. mutans'a karşı antimikrobiyal aktivite, Minimum İnhibisyon Konsantrasyonu ile belirlenmiştir. Tespit edilen Lactobacillus suşlarının süpernatant ve kültür ortamında biyofilm oluşturma yetenekleri araştırılmış, ayrıca α-amilaz toleransı ve pH değişim (24h-48h) kabiliyetleri incelenmiştir. L. plantarum, diğer Lactobacillus suşlarına kıyasla en yüksek antimikrobiyal aktiviteyi göstermiş, ayrıca L. plantarum, biyofilm oluşumunu inhibe etmiştir.

Inhibitory Effect of Probiotics Lactobacillus Supernatants Against Streptococcus Mutans and Preventing Biofilm Formation

Probiotic microorganisms can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. Dental caries is a multi-factorial chronic infection disease, which starts with bacterial biofilm formation caused mainly by S. mutans. This study investigated the characteristics of Lactobacillus spp. strains as an oral probiotic. Twelve Lactobacillus spp. species obtained from fermented milk and dairy products were identified. Antimicrobial activity was determined with the Minimum Inhibition Concentration against S. mutans as an oral pathogen. Biofilm formation capabilities of the identified Lactobacillus strains in supernatant and culture media were determined. In addition, their ability to α-amylase tolerance and pH values (24h-48h) were determined. L.plantarum showed the highest antimicrobial activity compare other Lactobacillus strains. Also, L. plantarum inhibited biofilm formation.

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