Karthik Madhayan, Sunder Suganya, Venkadachalam Balamurugan

Phytochemical Screening, Antioxidant and Anti-Inflammatory Properties of Litsea floribunda Gamble, Leaf and Bark Extract

The Litsea floribunda Gamble was widely used for various medicinal purposes. The present study was aimed to screening the phytochemicals presentin theleaf and bark extract of L. floribunda and also assess their antioxidant and anti-inflammatory properties. The results revealed that the leaf and bark extract showed the extracts are rich in phytomoleculeslikephenolics and tannin. The leaf extract showed more antioxidant activitiessuch as total antioxidant, ABTS, superoxide radical scavenging, FRAP, and DPPH reducing power activities were done respectively. Better radical activities Albumin denaturation and Anti-proteinase were observed in leaf extractthan in the bark extract, also it showed significant anti-inflammatory activities. Finally, L. floribunda could be considered as the most valuablealternative medicinalsource for many diseases

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Agarwal N, Chowdhary AS, Sharma MC, Dobhal MB. 2011. Chemical constituents of plants from the genus Litsea. ChemBiodivers, 8: 223-243.
Akgul H, Korkmaz N, Dayangaç A, Sevindik M. 2020. Antioxidant potential of endemic Salvia absconditiflora. Turkish Journal of Agriculture-Food Science and Technology, 8(10): 2222-2224.
Angel LJ, Mary KV. 2019. Phytochemical studies on Litsea floribunda (blume) gamble. (leaf). Adalya Journal, 8(9): 196- 202.
Angel LJ, Mary KV. 2020. GC-MS analysis on ethanolic flower extract of Litsea floribunda (BLUME) GAMBLE. Cikitusi Journal for Multidisciplinary Research, 7(1): 22-30.
Arnao MB, Cano A, Acosta M. 2011. The Hydrophilic and Lipophilic Contribution to Total antioxidant activity. Food Chemistry, 73(2): 239-244.
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N. 2019. Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants, 8: 96.
Balamurugan V, Raja K, Selvakumar S, Vasanth K. 2020. Phytochemical screening, antioxidant, anti-diabetic and cytotoxic activity of leaves of Pandanus canaranus Warb. Materials Today: Proceedings, 48: 322-329.
Benzie IF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239: 70-76.
Braca A, Tommasi ND, Bari LD, Pizza C, Politi, Morelli I. 2001. Antioxidant principles from Bauhunia terapotensis. Journal of Natural Products, 64: 892-895.
Cespedes CL, El-Hafidi M, Pavon N, Alarcon J. 2008. Antioxidant and cardioprotective activities of phenolic extracts from fruits of Chilean blackberry Aristotelia chilensis (Elaeocarpaceae), Maqui. Food Chemistry, 107: 820-829.
De- Fatima A, Modolo LV, Conegero LS, Pilli RA, Ferreira CV, Kohn LK, De- Carvalho JE. 2006. Lactones and their derivatives: biological activities, mechanisms of action and potential leads for drug design, Current Medicinal Chemistry, 13: 3371-3384.
Devika M, Nalini MS. 2018. In vivo evaluation of anxiolytic activity of aqueous and ethanolic extracts of Litsea floribunda (Bl.) Gamble –Lauraceae. Asian Journal of Pharmacy and Pharmacology, 4(1): 31-37.
Devika M, Joshi H, Nalini MS. 2016. Phytochemicals, Antioxidative and in vivo Hepatoprotective Potentials of Litsea floribunda (BL.) Gamble (Lauraceae)-An Endemic Tree Species of the Southern Western Ghats, India. Jordan Journal of Biological Sciences, 9(3).
Doss A. 2009. Preliminary phytochemical screening of some Indian medicinal plants. Ancient Science of Life, 29(2): 12-16.
Doss A. 2009. Preliminary phytochemical screening of some Indian medicinal plants. Ancient Science of Life, 29(2): 12- 16
Gamble JS, Fischer CEC. 1998. Flora of Presidency of Madras. Adlard and Son Limited 21, Hart street, WC Vol: 2.
Ghani A. 2003. Medicinal plants of Bangladesh: Chemical constituents and uses, Asiatic society of Bangladesh, Nimtali, Dhaka, 1000: 285.
Girish KE, Pradeep KG, Sasikala K, Shivadasan KK. 2014. Plants used in traditional herbal shampoos ( thaali) of Kerala, India; a documentation. Asia Pacific Journal of Research, 10: 56-63.
Hodge JE, Hofreiter BT. 1962. Determination of reducing sugars and carbohydrates. In: methods in carbohydrate chemistry, whistler, RL, ML wolfrom, JN Be-Miller and F Shafizadeh (Eds.). Academic Press Inc, New York USA, pp: 380-394.
Karthik Madhayan, Balamurugan Venkatachalam , Karthick Saravanan , Ananthi Palanisamy , Malarvizhi Annamalai. 2021. Comparative study of phytochemical, antioxidant and anti-inflammatory properties of ripe, semi ripe and unripe fruits of Phoenix pusilla Roxb. European Journal of Biotechnology and Bioscience, 9(3): 22-28.
Kına E, Uysal İ, Mohammed FS, Doğan M, Sevindik M. 2021. In-vitro antioxidant and oxidant properties of Centaurea rigida. Turkish Journal of Agriculture-Food Science and Technology, 9(10): 1905-1907.
Kirtikar K, Basu B. 1981. Indian medicinal plants (revised issue), vol. III, Allahabad, pp. 2156-61.
Koparde AA, Doijad RC, Magdum CS. 2019. Natural products in drug discovery. Pharmacognosy-medicinal plants, 1-20.
Korkmaz N, Dayangaç A, Sevindik M. 2021. Antioxidant, antimicrobial and antiproliferative activities of Galium aparine. Journal of Faculty of Pharmacy of Ankara University, 45(3): 554-564.
Lopez-Romero JC, Gonzalez-Rios H, Pena-Ramos A, Velazquez C, Navarro M, Robles-Zepeda R, Hernandez J. 2018. Seasonal effect on the biological activities of Litseag laucescens Kunth extracts. Evidence-Based Complementary and Alternative Medicine, 1-11.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193: 265-275.
Makkar HP. 2003. Quantification of tannins in tree and shrub foliage: a laboratory manual. Springer Science and Business Media Nov, 30.
Makkar HP, Siddhuraju P, Becker K. 2007. Plant secondary metabolites, Totowa: Humana Press Inc, pp 93-100.
Moor S, Stein WH. 1948. In methods in enzymol.Academic press, New York, pp 3: 468.
Nahak G, Sahu RK. 2010. In vitro antioxidant activity of Azaderachta indica and Meliaazedarach leaves by DPPH scavenging assay. Nature and Science, 6: 123- 128.
Nishikimi M, Rao NA, Yagi K. 1972. The occurrence of superoxide anion in the reaction of reduced phenazinemethosulfate and molecular oxygen. Biochemical and Biophysical Research Communications, 46: 849-854.
Pehlivan M, Mohammed FS, Şabik AE, Kına E, Dogan M, Yumrutaş Ö, Sevindik M. 2021. Some Biological activities of ethanol extract of Marrubium globosum. Turkish Journal of Agriculture-Food Science and Technology, 9(6): 1129-1132.
Pisoschi AM, Negulescu GP. 2011. Methods for total antioxidant activity determination: a review. Biochemistry Analytical Biochemistry, 1(1): 106.
Pradeepa K, Krishna V, Kumar KG, Thirumalesh BV, Kumar KN. 2011. Antibacterial screening of the stem bark and leaf extracts of Litsea glutinosa (Lour.) CB Rob-an ethnomedicinally important tree of the Western Ghats. Pharmacognosy Journal, 3(21): 72-76.
Prieto P, Pineda M, Aguilar M. 1999. Spectrophotometric quantification of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application of vitamin-E. Analytical biochemistry, 269: 337-341.
Rout SD, Thatoi HN. 2009. Ethnomedicinal practices of KoltribesinSimilipal Biosphere Reserve, Orissa, India.Ethnobotanical Leaflets, 13: 379-387.
Rout SK, Kar DM, Rout AB. 2012. Study of central nervous system activity of leaf extracts of neriumoleanderin experimental animal models. International Journal of Pharmacy and Pharmaceutical Sciences, 4(4): 378-382.
Sakat S, Juvekar AR, Gambhire MN, 2010. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn.International Journal of Pharmacy and Pharmaceutical Sciences, 2: 146-155.
Saldanha CJ. 1996. Flora of Karnataka. Oxford and IBH publishing Ltd, New Delhi, Vol: 1.
Saraswathy MK, Kalimuthu K, Chinnadurai V, Sharmila Juliet Y. 2017. In vitro Evaluation of Antidiabetic and Anti- inflammatory Activity of Ethanolic and Methanolic Extracts of Ceropegia juncea. British Journal of Pharmaceutical Research, 15: 1-9.
Sevindik M, Akgul H, Pehlivan M, Selamoglu Z. 2017. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull, 26(7): 4757-4763.
Shilpa K, Nimmy C, Prerana S. 2018. Investigation of anti- arthritic activity (in-vitro models) of Hibiscus hispidissimus Griffith. The Journal of Phytopharmacology, 7(1): 60-65.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. 2011. Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia, 98-106
Uysal İ, Mohammed FS, Şabik AE, Kına E, Sevindik M. 2021. Antioxidant and Oxidant status of medicinal plant Echium italicum collected from different regions. Turkish Journal of Agriculture-Food Science and Technology, 9(10): 1902- 1904.
Vasu K, Goud JV, Suryam A, Chary MAS. 2009. Biomolecular and phytochemical analyses of three aquaticangiosperms. African Journal Microbiology Research, 3(8):418-421.
Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64: 555-559.