FERBAL ÖZKAN YILMAZ, Zülfiye SU, ARZU ÖZLÜER HUNT, Metin YILDIRIM, SERAP YALIN

Nil Tilapyası, Oreochromis niloticus (Linnaeus, 1758) Karaciğer Dokusunda Kurşunun Neden Olduğu Oksidatif Strese Karşı Humik Maddelerin Koruyucu Etkisi

Bu araştırmada, subletal kurşun (Pb) derişiminin Nil tilapyası (Oreochromis niloticus)’nda antioksidan enzim sistemi üzerine etkilerine karşı, yeme katılan humik maddenin koruyucu etkisi incelenmiştir. Bu amaçla 1,5 mg/L kurşun nitrat Pb(NO3)2 ortam derişimi etkisindeki balıklar, %0,2 oranında humik madde katkısı bulunan yem ile beslenmişlerdir. Kontrol grubu, humik madde (HM), Pb, ve Pb+HM grublarını içeren bu çalışmada 4. ve 10. günlerde karaciğer doku örnekleri alınmıştır. Karaciğer dokusu katalaz (CAT) ve süperoksit dismutaz (SOD) enzim aktiviteleri ve lipid peroksidasyon ürünü olan malondealdehit (MDA) seviyesi, kontrol grubu ile karşılaştırıldığında, subletal kurşun derişimi uygulanan grupta 4. ve 10. günlerde önemli oranda arttığı belirlenmiştir. Pb grubu ile HM+Pb grubu karşılaştırıldığında, CAT ve SOD aktivitesinde önemli bir değişime neden olmadığı, ancak humik madde uygulamasının karaciğer dokusu lipid peroksidasyonu üzerine Pb toksisitesinin etkisini azalttığı belirlenmiştir. Deneme sonuçlarına göre, yeme ilave edilen %0,2 humik madde, 1,5 mg/L Pb ortam derişiminin, O. niloticus karaciğer dokusu lipid peroksidasyon üzerindeki toksisitesini belirli düzeyde azalttığı belirlenmiştir.

Protective Effect of Humic Substances Against Oxidative Stress Caused by Lead in Liver of Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758)

In this study, the protective effect of humic substance in the fish feed was investigated against the effects of subletal lead (Pb) concentration on the antioxidant enzyme system in Oreochromis niloticus. For this purpose, the fish which were affected by 1.5 mg/L lead nitrate Pb(NO3)2 medium concentration were fed with feed containing 0.2% of humic substance. Liver samples were taken at 4th and 10th days in this study, which included control group, humic substance (HM), Pb, and Pb + HM groups. The enzyme activity of liver tissue catalase (CAT) and superoxide dismutase (SOD) of O. niloticus and the level of malondialdehyde (MDA), a product of lipid peroxidation, increased significantly at 4th and 10th days in the group with subletal lead concentration compared with the control group. Compared with Pb and HM+Pb groups, it was determined that humic substance application decreased Pb toxicity on lipid peroxidation of liver tissue and did not cause a significant change in CAT and SOD activity. According to the results of the experiment, 0.2% humic substance added to the feed reduced toxicity of subletal Pb concentration on liver tissue lipid peroxidation.

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