DURALİ DANABAŞ, Mehmet KOCABAŞ, Filiz KUTLUYER

Improvement of Sperm Motility of Rainbow Trout (Oncorhynchus mykiss W., 1792) by Supplementation of L-Arginine

In this study, the effect of supplementing amino acid-precursor of nitric oxide (NO) L-arginine were examined on sperm motility of rainbow trout Oncorhynchus mykiss. Different concentrations [0 mM (Control), 1 mM, 2 mM, 4 mM and 8 mM] of L-arginine were used in the study. To assess the effects on percentage of motile sperm and longevity, L-arginine was added to activation medium containing NaCl (52 mM). The higher L-arginine concentration (4mM) has promoting-effect on sperm motility. The treatments containing L-arginine caused significant effect on percentage of motile sperm and longevity. Overall, the findings of the present study indicated that supplementation of L-arginine may have improving effects on sperm motility of rainbow trout.

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Aas GF, Refsie T, Gjerde B. 1991. Evaluation of milt quality of Atlantic salmon. Aquaculture, 95: 125-132.

Aydin S, Inci O, Alagol B. 1995. The role of arginine in domethacin and kallikrein in the treatment of oligospermia. IntUrolNephrol., 27:199–202.

Bozkurt Y, Öğretmen F, Kökçü Ö, Erçin U. 2011. Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: With emphasis on sperm motility. Czech J Anim Sci., 56(8): 355–364.

Buzadzic B, Vucetic M, Jankovic A, Stancic A, Korac A, Korac B, Otasevic V. 2015. New insights into male (in) fertility: The importance of NO. Brit J Pharmacol., 172(6): 1455–1467.

Ciereszko A, Dabrowski K. 1993. Estimation of sperm concentration of Rainbow trout, Whitefish and Yellow perch using a spectrophotometric technique. Aquaculture, 109: 367- 373.

Hajirezaee S, Amiri BM, Mirvaghefi A. 2010. Fish milt quality and major factors influencing the milt quality parameters: A review. African Journal of Biotechnology, 9(54): 9148-9154

Hassanpour H, Mirshokrai P, Shirazi A, Aminian A. 2007. Effect of nitricoxide on ram sperm motility in vitro. Pak J BiolSci PJBS., 10(14): 2374-2378.

Hassanpour H, Teshfam M, Karimi Goodarzi A, Tajik P, Mirshokraei P. 2010. In vitro effects of L-arginine on motion parameters in ram epididymal sperm. CompClinPathol., 19(4): 351-355.

Hellstrom WJG, Bell M, Wang R, Sikka SC. 1994. Effect of sodium nitroprusside on sperm motility, viability, and lipid peroxidation. Fert Steril., 61: 1117–1122.

Herrero MB, Cebral E, Boquet M, Viggiano JM, Vitullo A, Gimeno MA. 1994. Effect of nitricoxide on Mouse sperm hyperactivation. Acta Physiol Pharmacol Ther Latinoam., 44: 65–69.

Iaffaldano N, Di Iorio M, Manchisi A, Gibertoni PP, Esposito S. 2016. Semen quality of threatened native population of Mediterranean brown trout (Salmo cettii, Rafinesque 1810) in the Biferno River (Molise Region - South Italy). Turk J Fish Aquat Sci., 16: 259-266.

Inan SY, Yalcin I, Aksu F. 2004. Dual effects of nitricoxide in the Mouse forced swimming test: Possible contribution of nitricoxide-mediated serotonin release and potassium channel modulation. Pharmacol Biochem Behav., 77(3): 457-464.

Kocabas M, Can E, Kutluyer F, Aksu Ö. 2011. The reproduction traits of native brown trout (Salmo trutta macrostigma T., 1954). J Anim Vet Adv., 10(13): 1632-1637.

Kocabas M, Can E, Kızak V, Aksu Ö, Kutluyer F, Serdar O. 2012. Seasonal variation in food preference of the brown trout Salmo trutta macrostigma (T., 1954) from Uzungöl Stream, Turkey. Afr JAgricultRes., 7(13): 1982-1987.

Kocabas M, Kutluyer F. 2017a. In vitro effect of zinc: Evaluation of the sperm quality of endangered trout Salmo coruhensis and rainbow trout Oncorhynchus mykiss and fertilizing capacity. Int J Aquac Fish Sci., 3(2): 046-050.

Kocabas M, Kutluyer F. 2017b. Effect of Cobalt on sperm motility in an endangered trout species, Salmo coruhensis. Bull Environ Contam Toxicol., 99(6): 690-694

Kocabas M, Kutluyer F. 2017c. Dose dependent treatment with boric acid induces more changes in the sperm cells of endangered trout Salmo coruhensis and rainbow trout Oncorhynchus mykiss. J SciEngin Res., 4(9): 475-481.

Kocabas M, Kutluyer F, Erişir M, Benzer F. 2018. L-Arginine improves sperm motility in threatened Coruh Trout Salmo coruhensis (Teleostei: Salmonidae). Iranian Journal of Science and Technology, Transactions A: Science, Doi:https://doi.org/10.1007/s40995-018-0648-z.

Kutluyer F, Erisir M, Benzer F, Ögretmen F, Inanan BE. 2015. The in vitro effect of Lambda-cyhalothrin on quality and antioxidant responses of rainbow trout Oncorhynchus mykiss spermatozoa. Environ Toxicol Pharmacol 40: 855-860.

Kutluyer F, Benzer F, Erisir M, Ögretmen F, Inanan BE. 2016. The in vitro effect of cypermethrin on quality and oxidative stress indices of rainbow trout Oncorhynchus mykiss spermatozoa. Pest Biochem Physiol 128: 63-67.

Kutluyer F, Kocabas M. 2017. Dose dependent treatment with boric acid induces more changes in the sperm cells of endangered Anatolian trout Salmo rizeensis. Int J Aquac Fish Sci., 3(2): 042-045.

Lefièvre L, Chen Y, Conner SJ, Scott JL, Publicover SJ, Ford WCL. 2007. Human spermatozoa contain multiple targets for protein S-nitrosylation: An alternative mechanism of the modulation of sperm function by nitric oxide? Proteomics, 7: 3066–3084.

Patel AB, Srivastava S, Phadke RS, Govil G. 1998. Arginine activates glycolysis of goat epididymal spermatozoa: An NMR study. Biophy J., 75: 1522–1528.

Patel AB, Srivastava S, Phadke RS, Govil G. 1999. Arginine acts as a protective and reversal agent against glycolytic inhibitors in spermatozoa. Physiol Chem Phys Med NMR., 31: 29–40.

Piironen J, Hyvarinen H. 1983. Composition of the milt of some teleost fishes. Journal of Fish Biology, 22(3): 351-361, DOI: https://doi.org/10.1111/j.1095-8649.1983.tb04757.x.

Revelli A, Costamagna C, Moffa F, Aldieri E, Ochetti S, Bosia A, Massobrio M, Lindblom Bo, Ghigo D. 2001. Signaling pathway of nitric oxide-induced acrosome reaction in human spermatozoa. Biol Reprod., 64(6): 1708-1712.

Rurangwa E, Kime DE, Ollevier F, Nash JP. 2004. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234: 1–28,

Santana PPB, da Silva BB, Silva TVG, Costa NN, Cordeiro MS, Santos SSD, Ohashi OM, Miranda MS. 2016. Addition of Larginine to the fertilization medium enhances subsequent bovine embryo development rates. Theriogenology, 85: 1132–1138.

Salgueiroa RB, Gerlinger-Romero F, Guimarães-Ferreira L, de Castro Barbosa T, Nunes MT. 2017. Exercise training reverses the negative effects of chronic L-arginine supplementation on insulin sensitivity. Life Sci., 191: 17–23.

Scott GS, Bolton C. 2000. L-Arginine modifies free radical production and the development of experimental allergic encephalomyelitis. InflamRes., 49: 720-726.

ShikhMaidin M, Adanana NF, Aminudin MT, Tawang A. 2014. In vitro supplements improves motility and progressive score of spermatozoa in jermasia goats. APCBEE Proc., 8: 329- 333.

Srivastava S, Prashant D, Coutinho E,Govil G. 2006. Mechanism of action of L-Arginine on the vitality of spermatozoa is primarily through increased biosynthesis of nitric oxide. Biol Reprod., 74: 954–958.

Suquet M, Billard R, Cosson J, Dorange G, Chauvaud L, Mugnier C, Fauvel C. 1994. Sperm features in turbot (Scophthalmus maximus): A comparison with other freshwater and marine fish species. Aquat. Living Resour., 7: 283-294.

Tekin N, Seçer S, Akçay E, Bozkurt Y, Kayam S. 2003. The effect of age on spermatological properties in rainbow trout (Oncorhynchus mykiss W., 1792). Turkish Journal of Veterinary and Animal Sciences, 27: 37-44.

Wilson-Leedy JG, Ingermann RL. 2011. Production of nitric oxide by sperm of the steelhead (Oncorhynchus mykiss) and its actions on motility and respiration. Theriogenology, 75: 144–154.

Yeoman RR, Jones WD, Rizk BM. 1998. Evidence for nitric oxide regulation of hamster sperm hyperactivation. J Androl., 19: 58–64.

Zhang H, Zheng RL. 1996. Possible role of nitric oxide on fertile and as the no zoospermic in fertile human sperm functions. Free Rad Res., 25: 347–354.