İki Calendula Türünde Belirli Fenolik Bileşiklerin ve Karotenoidin Hplc ile Analizi

Asteraceae familyasına ait, tıbbi amaçla kullanılan bir süs bitkisi olan Calendula officinalis (tıbbi nergis, aynısefa) antikanser, antimikrobiyal, antioksidan, antitümör gibi çeşitli tıbbi aktivitelere sahiptir. HPLC ile analizi yapılması planlanan kuersetin (6,445 dak), kaemferol (11,246 dak), kafeik asit (2,333 dak), beta karoten (9,614 dak) standardlarının en iyi piki ve en fazla miktarı verdiği tutulma zamanı belirlenmiştir. Bu metabolitler için en uygun olan HPLC koşulları saptanmıştır. Bu iki Calendula türünün 0., 15., 30., 45., 60. günlerindeki fide yapraklarındaki kuersetin, kaemferol, kefeik asit ve beta karoten miktarları karşılaştırılmıştır. Yapraklarda kafeik asit ve beta karoten tespit edilmiştir. Fakat kuersetin ve kaemferol bileşikleri tespit edilememiştir. C. officinalis türünün kafeik asit ve beta karoten miktarının, C. arvensis türüne göre daha fazla olduğu belirlenmiştir. C. officinalis ve C. arvensis fidelerinin her ikisinde de en yüksek kafeik asit (C. officinalis için 0,4042±0,0123 µg/g kuru ağırlık, C. arvensis için 0,1918±0,0437 µg/g kuru ağırlık) ve beta karoten miktarları (C. officinalis için 0,8520±0,0692 µg/g kuru ağırlık, C. arvensis için 0,6389±0,0189 µg/g kuru ağırlık) 60. günde saptanmıştır. Ayrıca fide gelişim dönemine göre, bu metabolitlerin miktarının farklılık gösterdiği saptanmıştır.

Hplc Analysis of Certain Phenolic Compounds and Carotenoid in Two Calendula Species

Calendula officinalis (pot marigold), a medicinal ornamental plant belonging to the Asteraceaefamily, has various medicinal activities such as anticancer, antimicrobial, antioxidant and antitumor.The retention time of quercetin (6.445 min), kaempferol (11.246 min), caffeic acid (2.333 min), betacarotene (9.614 min) standards, which were planned to be analyzed by HPLC, was determined. TheHPLC conditions that are most suitable for these metabolites have been determined. The amounts ofquercetin, kaempferol, caffeic acid and beta carotene in the seedling leaves of these two Calendulaspecies on the 0., 15., 30., 45., 60. days were compared. Caffeic acid and beta carotene have beendetected in the leaves. However, quercetin and kaempferol compounds were not detected. It wasdetermined that the caffeic acid and beta carotene amounts of C. officinalis species were higher thanthat of C. arvensis. The highest caffeic acid in both C. officinalis and C. arvensis seedlings(0.4042±0.0123 µg/g dry weight for C. officinalis, 0.1918±0.0437 µg / g dry weight for C. arvensis)and beta carotene amounts (0.8520±0.0692 µg/g dry weight for C. officinalis, 0.6389±0.0189 µg/gdry weight for C. arvensis) were determined on 60. day. In addition, it was determined that the amountof these metabolites differed according to the seedling development period.

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