Dilek DEMİREL ŞENTÜRK, RAMAZAN DEMİREL, Muzaffer Aydın KETANİ, Kadri BALCI

Effects on Performance, Skin and Liver Histology of Different Clinoptilolite Levels in Rat Diets#

Bu araştırma rat yemlerine ilave edilen doğal zeolitin (klinoptilolit) performans, deri ve karaciğer histolojisi üzerine etkilerini incelemek amacıyla yapılmıştır. Bu çalışmada, hayvan materyali olarak 24 adet on haftalık yaştaki, yaklaşık 306  18,93 g canlı ağırlığındaki sütten kesilmiş erkek yetişkin Spraque-Dawley rat kullanılmıştır. Ratlar tesadüf parselleri deneme desenine göre üçer tekerrürlü, 4 gruba (klinoptilolit içermeyen grup kontrol grubunu oluştururken, %2, %4, and %6 düzeylerinde klinoptilolit ilavesinin yapıldığı gruplar muamele gruplarını oluşturacak şekilde) rastgele dağıtılmıştır. Deneme 56 gün sürmüştür. Deneme bireysel paslanmaz çelikten yapılmış kafeslerde yürütülmüştür. Primer ve sekonder folikül sayıları bakımından gruplar arasında istatistiki olarak önemli düzeyde farklılık tespit edilmemekle beraber, her iki folikülün çap değerleri ise önemli bulunmuştur. Primer ve sekonder folikül sayıları ve çapları en yüksek ve en düşük değerler olarak sırasıyla 2,00-2,33; 4,50-7,17 ve 11,53-20,42; 57,63- 102,12m, olarak tespit edilmiştir. Önemli düzeydeki farklılık control grubuyla, IV. grup (%6 klinoptilolit içeren) arasında meydana gelmiştir. Ayrıca, derinin ve karaciğerin histolojik olarak incelenmesi sonucunda gruplar arasında önemli düzeyde farklılık tespit edilmemiştir.

Rat Rasyonlarında Farklı Düzeylerde Klinoptilolit Kullanımının Performans, Deri ve Karaciğer Histolojisi Üzerine Etkileri

The objective of this study was to test the effects of dietary natural zeolite (clinoptilolite) on performance, skin and liver histology in rats. In this study, 24 10-week-old, weaned, adult male Spraque-Dawley rats with approximately 306  18.93 g initial live weight were used. The rats were randomly divided into four groups with three replicates, including a control group and groups with one of three doses of clinoptilolite (2%, 4%, and 6%) in their diets. All the rats were fed these concentrates throughout the experimental period of 56 days. The animals were reared individually in stainless steel cages. There were no significant differences in the primary and secondary follicle numbers among groups, but the diameters of each follicle were found to be significant. The primary and secondary follicle numbers and diameters ranged from lowest to highest as follows: 2.00-2.33, 4.50-7.17; and 11.53-20.42, 57.63-102.12m, respectively. The differences occurred between the control group and group IV (containing 6% zeolite). In addition, the skin and liver histology results showed that there were no differences among the groups.

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