Determination of Heavy Metals in Locally Available Chocolates in Lahore Region

Heavy metal toxicity can either be acute or chronic effects. Long-term exposure of the body to heavy metal can progressively lead to muscular, physical and neurological degenerative process. In this research, a total of 30 representative chocolate samples were collected from local shops and markets in Lahore. All the samples were analysed to assess the levels of Lead (Pb), Nickel (Ni), Chromium (Cr) and Cadmium (Cd) by using Atomic Absorption Spectrophotometer in Pakistan Council of Scientific and Industrial Research (PCSIR), Lahore. It was observed that the majority of chocolate samples contain heavy metals levels higher than the permissible limits as recommended by Punjab Food Rules (PFR) 2011. The levels of Pb ranged between 0.375 and 3.4 mg/kg with 90% samples having concentrations exceeding PFR allowable limit (0.5 mg/kg). Whereas, Ni concentrations varied from 0.005 to 0.28 mg/kg and 80% samples were above the PFR limit (0.025 mg/kg). Further, Cr levels analysed between 0.005 to 0.28 mg/kg and 53% samples had Cr concentrations higher than PFR limit (0.02 mg/kg). Similarly, Cd levels were observed between 0.50-3.25 mg/kg with 53% samples having Cd concentrations exceeding the PFR limit (1.0 mg/kg). Investigation indicates that the quality of available chocolates is not recommendable for eating because of the higher concentrations of toxic heavy metals.

___

Akhtar, S. 2015. Food safety challenges—a Pakistan’s perspective. Critical reviews in food science and nutrition, 55(2): 219-226.

Abnet C. C. 2007. Carcinogenic food contaminants. Cancer investigation, 25(3): 189-196.

Alagić N, Huremović J. 2015. Determination of metal contents in various chocolate samples. Glas. Hem. Technol. Bosne Herceg, 45: 39-42.

Abt E, Robin L. P. 2020. Perspective on cadmium and lead in cocoa and chocolate. Journal of agricultural and food chemistry, 68(46): 13008-13015.

Amjad M, Hussain S, Javed K, Khan A. R, Shahjahan, M. 2020. The Sources, Toxicity, Determination of Heavy Metals and Their Removal Techniques from Drinking Water. World, 5(2): 34-40.

Clemens S Ma J F. 2016. Toxic heavy metal and metalloid accumulation in crop plants and foods. Annual review of plant biology, 67: 489-512.

Dahiya S, Karpe R, Hegde A, Sharma R. 2005. Lead, cadmium and nickel in chocolates and candies from suburban areas of Mumbai, India. Journal of Food Composition and Analysis, 18(6): 517-522.

Dapul H, Laraque D. 2014. Lead poisoning in children. Advances in pediatrics, 61(1): 313-333.

Devi P, Bajala V, Garg V. Mor S, Ravindra K. 2016. Heavy metal content in various types of candies and their daily dietary intake by children. Environmental monitoring and assessment, 188(2): 86.

Dohnalova L, Bucek P, Vobornik P, Dohnal V. 2017. Determination of nickel in hydrogenated fats and selected chocolate bars in Czech Republic. Food chemistry, 217, 456-460.

Godbersen L, Duijnisveld W, Utermann J, Gäbler H, E, Kuhnt G, Böttcher, J. 2012. Application of groundwater thresholds for trace elements on percolation water: a case study on percolation water from Northern German Lowlands. Journal of environmental quality, 41(4): 1253-1262.

Goldstee R, Goldsteen K, Dwelle T. 2014. Introduction to Public Health: Promises and Practice: Springer Publishing Company.

Fong I. 2017. Animals and Mechanisms of Disease Transmission. Emerging Zoonoses, 15-38.

Gizaw Z. 2019. Public health risks related to food safety issues in the food market: a systematic literature review. Environmental health and preventive medicine, 24(1): 1-21.

Hezbullah M, Sultana S, Chakraborty S, Patwary M. 2016. Heavy metal contamination of food in a developing country like Bangladesh: An emerging threat to food safety. Journal of Toxicology and Environmental Health Sciences, 8(1): 1-5.

Iwegbue C. M. 2011. Concentrations of selected metals in candies and chocolates consumed in southern Nigeria. Food additives and Contaminants, 4(1): 22-27.

Iwegbue C. M, Nwozo S. O , Overah C. L, Bassey F. I, Nwajei, G. E. 2013. Concentrations of selected metals in some readyto-eat-foods consumed in southern Nigeria: estimation of Dietary intakes and target hazard quotients. Turkish Journal of Agriculture-Food Science and Technology, 1(1): 1-7.

Jalbani N, Kazi T. G, Afridi H. I, Arain, M. B. 2009. Determination of toxic metals in different brand of chocolates and candies, marketed in Pakistan. Pakistan Journal of Analytical & Environmental Chemistry, 10(1 & 2): 5.

Järup L. 2003. Hazards of heavy metal contamination. British medical bulletin, 68(1): 167-182.

Kirk M. D, Pires S. M, Black R. E, Caipo M, Crump J. A, Devleesschauwer B, Döpfer D, Fazil A, Fischer-Walker C. L, Hald T. 2015. World Health Organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal, and viral diseases, 2010: a data synthesis. PLoS medicine, 12(12): e1001921.

Kruszewski B, Obiedziński M. W, Kowalska, J. 2018. Nickel, cadmium and lead levels in raw cocoa and processed chocolate mass materials from three different manufacturers. Journal of Food Composition and Analysis, 66: 127-135

Kanmani S, Gandhimathi R. 2013. Assessment of heavy metal contamination in soil due to leachate migration from an open dumping site. Applied Water Science, 3(1): 193-205.

Lari S. Z, Khan N. A, Gandhi K N, Meshram T. S, Thacker N. P. 2014. Comparison of pesticide residues in surface water and ground water of agriculture intensive areas. Journal of Environmental Health Science and Engineering, 12(1): 1-7.

Leiter J, Harding S. 2004. Trinidad, Brazil, and Ghana: three melting moments in the history of cocoa. Journal of rural Studies, 20(1): 113-130.

Montes-Santiago J. 2013. The lead-poisoned genius: saturnism in famous artists across five centuries. Progress in brain research, 203: 223-240.

Malakootian M, Nekoie Moghdam M, Jannati A, Pourshaaban Mazandarany M. 2012. Determination of Lead Levels in Candy, Chocolate and Chewing Gum Existent in Kerman in 2009 and Compare it’s by FDA Standard: A Short Reaport. Journal of Rafsanjan University of Medical Sciences, 11(2): 179-184.

Mohammed FS, Akgul H, Sevindik M, Khaled BMT. 2018. Phenolic content and biological activities of Rhus coriaria var. zebaria. Fresenius Environmental Bulletin, 27(8): 5694- 5702.

Mohammed FS, Pehlivan M, Sevindik M. 2019. Antioxidant, antibacterial and antifungal activities of different extracts of Silybum marianum collected from Duhok (Iraq). International Journal of Secondary Metabolite, 6(4): 317-322.

Mohammed FS, Günal S, Şabik AE, Akgül H, Sevindik M. 2020. Antioxidant and Antimicrobial activity of Scorzonera papposa collected from Iraq and Turkey. Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, 23(5): 1114-1118.

Nordberg G. F, Nogawa K, Nordberg, M. 2015. Cadmium Handbook on the Toxicology of Metals. UK: Academic Press. ISBN 978-0-12-398292-6

Odeyemi O. A, Sani N. A, Obadina A. O, Saba C. K. S, Bamidele F. A, Abughoush M, Asghar A, Dongmo F. F. D, Macer D, Aberoumand A. 2019. Food safety knowledge, attitudes and practices among consumers in developing countries: An international survey. Food research international, 116: 1386- 1390.

Ochu J, Uzairu A, Kagbu, J, Gimba C, Okunola O. 2012. Evaluation of Some Heavy Metals in Imported Chocolate and Candies Sold in Nigeria. Journal of Food Research, 1(3): 169.

Olmedo P, Pla A, Hernández A, Barbier F, Ayouni L, Gil, F. 2013. Determination of toxic elements (mercury, cadmium, lead, tin and arsenic) in fish and shellfish samples. Risk assessment for the consumers. Environment international, 59: 63-72.

Ramtahal G, Yen I. C, Bekele I, Bekele F, Wilson L, Sukha B, Maharaj K. 2015. Cost-effective method of analysis for the determination of cadmium, copper, nickel and zinc in cocoa beans and chocolates. Journal of Food Research, 4(1):193.

Rehman S, Husnain, S. M. 2012. Assessment of trace metal contents in chocolate samples by Atomic Absorption Spectrometry. J. Trace Elem. Anal, 1(1): 1-11.

Rivas L, Mellor G. E, Gobius K, Fegan, N. 2015. Detection and Typing Strategies for Pathogenic Escherichia coli. Australia: Springer New York Heidelberg Dordrecht London. ISBN 978-1-4939-2345-8

Sankhla M. S, Kumar, R. 2019. Contaminant of Heavy Metals in Groundwater & its Toxic Effects on Human Health & Environment, 18 (5): 01-05.

Saha R, Nandi R, Saha B. 2011. Sources and toxicity of hexavalent chromium. Journal of Coordination Chemistry, 64(10): 1782-1806.

Salama A. K. 2018. Health risk assessment of heavy metals content in cocoa and chocolate products sold in Saudi Arabia, 38(4): 318-327

Sevindik M, Akgul H, Pehlivan M, Selamoglu Z. 2017. Determination of therapeutic potential of Mentha longifolia ssp. longifolia. Fresen Environ Bull, 26(7): 4757-4763.

Sevindik M. 2019. The novel biological tests on various extracts of Cerioporus varius. Fresenius Environmental Bulletin, 28(5): 3713-3717.

Temiz H, Soylu A. 2012. Heavy metal concentrations in raw milk collected from different regions of Samsun, Turkey. International journal of dairy technology, 65(4): 516-522.

Turan G. Y. M, Akccedil F. 2011. The importance of Turkish hazelnut trace and heavy metal contents for human nutrition. Journal of Soil Science and Environmental Management, 2(1): 25-33.

Verna R. 2013. The history and science of chocolate. The Malaysian journal of pathology, 35(2): 111.

Villa J. E, Peixoto R. R, Cadore, S. 2014. Cadmium and lead in chocolates commercialized in Brazil. Journal of agricultural and food chemistry, 62(34): 8759-8763.

Yanus R. L, Sela H, Borojovich E. J, Zakon Y, Saphier M, Nikolski A, Karpas Z. 2014. Trace elements in cocoa solids and chocolate: an ICPMS study. Talanta, 119(15):1-4.

Zeinali T, Salmani F. Naseri K. 2019. Dietary intake of cadmium, chromium, copper, nickel, and lead through the consumption of meat, liver, and kidney and assessment of human health risk in Birjand, Southeast of Iran. Biological trace element research, 191(2): 338-347.
Türk Tarım - Gıda Bilim ve Teknoloji dergisi-Cover
  • ISSN: 2148-127X
  • Yayın Aralığı: Aylık
  • Başlangıç: 2013
  • Yayıncı: Turkish Science and Technology Publishing (TURSTEP)