Elçin TAŞ, ZERRİN ERGİNKAYA, SELİN KALKAN, EMEL ÜNAL TURHAN

Determination of Antimicrobial Effects of Probiotic Lactic Acid Bacteria and Garlic Extract Against Some Foodborn Pathogenic Bacteria

In this study, it was investigated that the inhibition effect of some lactic acid bacteria (Lactobacillus acidophilus NCC68, Lactobacillus casei Shirota, Lactobacillus rhamnosus (Ezal, commercial starter cultures)) which possessed with probiotic characteristics, against Bacillus cereus, Salmonella Enteritidis, Escherichia coli, Escherichia coli 0157:H7 ATCC 35150 and Staphylococcus aureus ATCC 25923. Besides, the inhibitory effect of probiotic cultures which used with meat and meat product additives that garlic extract over the antagonistic effects of sensitive pathogens were investigated in vitro. Consequently, the whole of lactic acid bacteria and garlic extract which were used in this study, showed inhibition effects against all selected pathogenic bacteria. Staphylococcus aureus ATCC 25923 was determined as the most sensitive pathogenic bacteria while Bacillus cereus was the most resistant bacteria against lactic acid bacteria and garlic extract. There was a distinctive increase in inhibition effects were observed by used of a combination with lactic acid bacteria and garlic extract.

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Andersson A, Rfnner U, Granum PE. 1995. What problems does the food industry have with the spore-forming pathogens Bacillus cereus and Clostridium perfringens?. Int. J. of Food Microbiol. 28: 145– 155.

Arora DS, Kaur J. 1999. Antimicrobial activity of species. Int. J. Antimicrobial Agents. 12: 257-262.

Arqués JL, Rodríguez E, Langa S, Landete JM, Medina M. 2015. Antimicrobial activity of lactic acid bacteria in dairy products and gut: effect on pathogens. BioMed Research Int. Article ID 584183. 9 pages.

Aslım B, Yüksekdağ ZN, Sarıkaya E, Beyatlı, Y, 2005. Determination of the bacteriocin-like substances produced by some lactic acid bacteria isolated from Turkish dairy products. LWT. 38: 691-694.

Benkeblia N. 2004. Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). LWT. 37: 263-268.

Boyce TG, Pemberton AG, Wells JG, Griffin PM. 1995. Screening for Escherichia coli O157: H7-a nationwide survey of clinical laboratories. J. Clin. Microbiol. 33(12): 3275-3277.

Casella S, Leonardi M, Melai B, Fratini F, Pistelli L. 2013. The role of diallyl sulfides and dipropyl sulfides in the in vitro antimicrobial activity of the essential oil of garlic, Allium sativum L. and leek Alliumporrum L. Phytother. Res. 27: 380–383.

Chuayana Jr EL, Ponce CV, Rıvera MRB, Cabrera EC. 2003. Antimicrobial activity of probiotics from milk products. Phil. J. Microbiol. Dis. 32 (2): 71-74.

Cizeikiene D, Juodeikiene G, Paskevicius A, Bartkiene E. 2013. Antimicrobial activity of lactic acid bacteria against pathogenic and spoilage microorganism isolated from food and their control in wheat bread. Food Cont. 31: 539-545.

Daka D. 2011. Antibacterial effect of garlic (Allium sativum) on Staphylococcus aureus: An in vitro study. Afr. J. of Biotechnol. 10 (4): 666-669.

Del Castillo V, Goldner MC, Armada M. 2016. Evaluation of texture profile, color and determination of FOS in yacón products (Smallanthus sonchifolius). Turk. J. of Agriculture - Food Sci. and Technol.4(7): 540-544.

Ghanbari M, Jami M, Domig KJ, Kneifel W. 2013. Seafood biopreservation by lactic acid bacteria-A review. LWT-Food Sci. and Technol. 54: 315-324.

Gonzalez L, Sandovel H, Sacristan N, Castro JM, Fresno JM, Tornadijo ME. 2006. Identification of lactic acid bacteria isolated from genestoso cheese throughout ripening and study of their antimicrobial activity. Food Control. 18: 716-722.

Granum PE, 2001. Bacillus cereus. In: Doyle, M.P., Beuchat, L.R., Montville, T.J. (Eds.), Food Microbiology. Fundamentals and frontiers, second edition. ASM Press, Washington, DC. 373– 381 pp.

Gulfraz M, Imran M, Khadam S, Ahmed D, Asad MJ, Abassi KS, Irfan M, Mehmood S. 2014. A comparative study of antimicrobial and antioxidant activities of garlic extract in various localities of Pakistan. Afr. J. of Plant Sci. 8(6): 298-306.

Hayaloğlu AA, Erginkaya Z. 2001. Gıda endüstrisinde kullanılan laktik asit bakterileri. Gıda Teknolojileri Derneği Yayın No:23. Ankara.

Indu MN, Hatha AMM, Abirosh C, Harsha U, Vivekanandan G. 2006. Antimicrobial activity of some of the South-Indian spices against serotypes of Escherichia coli, Salmonella, Listeria monocytogenes and Aeromonas hydrophila. Brezillian J. Microbiol. 37:153-158.

Kumar M, Berwal JS. 1998. Sensitivity of food pathogens to garlic (Allium sativum). J. App. Microbiol. 84: 213-215.

Kumar Y, Agarwal S, Srivastava A, Kumar S, Agarwal G, Khan MZA. 2014. Antibacterial activity of clove (Syzygium aromaticum) and garlic (Allium sativum) on different pathogenic bacteria. Int. J. Pure App. Biosci. 2 (3): 305-311.

Kyung KH. 2012. Antimicrobial properties of allium species. Current Op. in Biotechnol. 23:142–147.

Larsen HD, Jørgensen K. 1997. The occurrence of Bacillus cereus in Danish pasteurized milk. Int. J. of Food Microbiol. 34: 179– 186.

Li G, Ma X, Deng L, Zhao X, Wei Y, Gao Z, Jia J, Xu J, Sun C. 2015. Fresh garlic extract enhances the antimicrobial activities of antibiotics on resistant strains in vitro. Jundishapur Microbiol. 8(5): 14-18.

Makras L, De Vuyst L. 2006. The in vitro inhibition of Gramnegative pathogenic bacteria by Bifidobacteria is caused by the production of organic acids. Int. Dairy J. 16: 1049-1057.

Mayr R, Eppert I, Scherer S. 1999. Incidence and identification of psychrotrophic (7 degrees C-tolerant) Bacillus spp. in German HTST pasteurized milk. Milchwissenschaft. 54: 26–30.

Meer RR, Baker J, Bodyfelt FW, Griffiths MW. 1991. Psychotropic Bacillus spp. in fluid milk-products-a review. J. of Food Pro. 54: 969-979.

Mukhtar S, Ghori I. 2012. Antibacterial activity of aqueous and ethanolic extracts of garlic, cinnamon and turmeric against Escherichia coli ATCC 25922 and Bacillus subtilis. 3(2): ISSN:0976-4550.

Nieto-Lozano JC, Raguera-Useros JI, Pelaez-Martinez MC, De La Torre AH. 2002. Bacteriocinogenic activity from starter cultures used in Spanish meat industry. Meat Sci. 62: 237-243.

Öz F, Kaya M, Aksu Mİ. 2002. Sucuk üretiminde farklı nitrit dozlarının ve starter kültür kullanımının Escherichia coli O157:H7'nin gelişimi üzerine etkisi. Türk J Vet. Anim. Sci. 26: 651-657.

Pascual MO, Hugas M, Badıola JI, Monfort JM, Garrıga M. 1999. Lactobacillus salivarius CTC2197 prevents Salmonella Enteritidis colonization in chickens. App. and Env. Microbiol. 65 (11): 4981–4986.

Philips CA, Duggan J. 2001. The effect of EDTA and trisodium phosphate, alone and in combination with nisin, on the growth of Arcobacter butzleri in culture. Food Microbiol. 18: 5547-554.

Ranjan S, Dasgupta N, Saha P, Madhumita Rakshit M, Cizeikiene R. 2012. Comparative study of antibacterial activity of garlic and cinnamon at different temperature and its application on preservation of fish. Adv. in App. Sci. Research. 3(1): 495-501.

Reis JA, Paula AT, Casarotti SN, Penna ALB. 2012. Lactic acid bacteria antimicrobial compounds: characteristics and applications. Food Eng Rev. 4:124–140.

Rossland E, Langsrud T, Sorhaug T. 2005. Influence of controlled lactic fermentation on growth and sporulation of Bacillus cereus in milk. Int. J. Food Microbiol. 103: 69-77.

Rossland E, Morge GIA, Langsrud T, Sorhaug T. 2003. Inhibition of Bacillus cereus by strains of Lactobacillus and Lactococcus in milk. Int. J. Food Microbiol. 89: 205-212.

Sameshima T, Magome C, Takeshita K, Arihara K, Itoh M, Kondo Y. 1998. Effect of intestinal Lactobacillus starter cultures on the behavior of Staphylococcus aureus in fermented sausages. Int. Journal of Food Microbiol. 41: 1-7.

Schillinger U, Lucke FK. 1989. Antimicrobial activity of Lactobacillus sake isolated from meat. App. and Env. Microbiol. 55(8): 1901-1906.

Singh B, Falahee MB, Adams MR. 2001. Synergistic inhibition of Listeria monocytıgenes by nisin and garlic extract. Food Microbiol. 18: 133-139.

Siroli L, Patrignani F, Serrazanetti D, Tabanelli G, Montanari C, Gardini F, Lanciotti R. 2015. Lactic acid bacteria and natural antimicrobials to improve the safety and shelf-life of minimally processed sliced apples and lamb's lettuce. Food Microbiol. 47: 74-84.

Sobrino O, Rodriguez JM, Moreira WL, Fernandez MF, Sanz B, Hernandez PE. 1991. Antibacterial activity of Lactobacillus sake isolated from dry fermented sausages. Int. J. Food Microbiol. 13: 1-10.