Ayşe Nur DEMİRCİ, NAZAN ÇÖMLEKCİOĞLU, Ashabil AYGAN

Cedrus libani ve Pinus nigra subsp. pallasiana’nın Uçucu Yağlarının Kimyasal Bileşimi, Antimikrobiyal Aktivitesi ve Flavonoid İçeriklerinin Belirlenmesi

Kahramanmaraş’ta doğal olarak yayılış gösteren Cedrus libani A. Rich ve Pinus nigra subsp. pallasiana (Lamb.) Holmboe ’nın iğne yaprak ve meyvelerine ait uçucu yağların bileşimi GC-MS ile; antimikrobiyal etkileri disk difüzyon yöntemi ile flavonoid içerikleri ise HPLC cihazı ile belirlenmiştir. Nisan, Temmuz ve Eylül olmak üzere üç farklı zamanda toplanan yaprak örneklerine ait uçucu yağların kimyasal kompozisyonundaki mevsimsel değişimin incelendiği bu çalışmada, uçucu yağ ana bileşenlerini P. nigra subsp. pallasiana’da α-pinene, β-pinene, limonene, βcaryophyllene ve germacrene-D ile; C. libanii’de bu bileşenlere ek olarak, myrcene ve α–terpineol olarak bulunmuştur. HPLC analizi sonucunda rutin, quercetin, kaempferol, naringin, ve resveratrol flavonoidleri çeşitli oranlarda bitkilerde bulunmuştur. C. libani’de en yüksek oranda rutin ve resveratrol (sırasıyla 154,33 ve 20,02 µg g- ) bulunurken; P. nigra subsp. pallasiana’da ise yine rutinin (39,66 µg g- ) yanı sıra quercetin ve naringin (sırasıyla 9,65 ve 9,31 µg g- ) elde edilmiştir. Antimikrobiyal aktivite sonuçlarına göre Nisan’da toplanan C. libani uçucu yağları Haziran ve Eylül ayı uçucu yağlarından daha yüksek aktivite göstermiştir. Buna karşılık P. nigra subsp. pallasiana uçucu yağları en iyi antimikrobiyal aktiviteyi Nisan ve Haziran’a kıyasla Eylül’de göstermiştir. Sonuç olarak, C. libani ve P. nigra subsp. pallasiana antimikrobiyal aktivite gösteren bir içeriğe sahiptir ancak en iyi ve etkin bir içerik için hasat dönemlerine dikkat edilmelidir.

Determination of the Chemical Composition, Antimicrobial Activity and Flavonoid Content of the Essential Oils of Cedrus libani and Pinus nigra subsp. pallasiana

Essential oil composition, antimicrobial activity and flavonoid contents of leaf-fruits of Cedrus libani and Pinus nigra subsp. pallasiana were determined with GC-MS, disc diffusion method and HPLC in three different period. When the essential oil composition of leaf and fruits of P. nigra ssp pallasiana collected in April, July and September, major components were α-pinene, β-pinene, limonene, β-caryophyllene ve germacrene-D. On the other hand, additionally to these contents, myrcene and α–terpineol were also detected in C. libani. According to HPLC analysis, rutin, quercetin, kaempferol, naringin and resveratrol flavonoids were detected in different proportions. While rutin (154.33 µg g- ) and resveratrol (20.02 µg g- ) has the highest ratio in C. libani, quercetin (9.65 µg g - ) and naringin (9.31 µg g- ) were detected in P. nigra subsp. pallasiana along with rutin (39.66 µg g - ). According to the antimicrobial activity results the essential oils of C. libani obtained in April has produced higher activity than that of July and September. On the contrary, the essential oils from P. nigra subsp. pallasina have produced the best antimicrobial activity on September compared to April and July. As a result, C. libani and P. nigra subsp. pallasiana essential oils have a composition showing antimicrobial activity and their harvesting season should be determined for the best and effective content.

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