MEHMET YAMAN, Hasan PINAR, Ubeyid SEDAY, Duygu ALTUNÖZ, AYDIN UZUN, Nur Selin ÇABUK

Bazı Turunçgil Anaçlarında Tuza Tolerans için In vitro Testleme

Turunçgiller coğrafi dağılımlarından dolayı tuzluluğa hassas bölgelerde yetiştirilen ürünler arasında önemli bir yerdedir. Testleme metotları, ıslah ve ürün geliştirme programlarında önemli bir konuma sahiptir. Yapılan bu çalışmada in vitro koşullarda Kleopatra mandarini (Citrus reshni Tan.), Turunç (Citrus aurantium L.), Kaba limon (Citrus jambhiri Lush.), Volkamer limon (Citrus volkameriana Tan & Pasq.), Carrizo sitranjı (Poncirus trifoliata L. Raf. × Citrus sinensis L. Osbeck) ve Üç yapraklı (Ponciru trifoliata Raf.) gibi bazı turunçgil anaçlarının farklı NaCl konsantrasyonlarında tuza olan tepkilerini belirlemek amaçlanmıştır. Söz konusu turunçgil anaçlarına ait tohumlar 0, 45, 90 ve 135 mM NaCl içeren MS ortamında in vitro tohum çimlendirilme testine tabi tutulmuştur. Genel olarak bütün tuz konsantrasyonlarında en yüksek çimlenme Volkamer Limon ve Turunç anacından elde edilirken, en düşük çimlenme Kaba limon ve Üç yapraklı anacında gerçekleşmiştir. Sonuç olarak bu çalışmayla turunçgil anaçlarında tuza toleransı belirlenmesinde in vitro koşulların hızlı testleme de kullanılabileceği kanısına varılmıştır.

In vitro Screening for Salt Tolerance of Some Citrus Rootstocks

Just because of geographical spread, citrus species generally grow in places sensitive to salinity. Testing methods have a significant role in breeding and cultivar development programs. This study was conducted to investigate in vitro salt response of Cleopatra mandarin (Citrus reshni Tan.), sour orange (Citrus aurantium L.), rough lemon (Citrus jambhiri Lush.), Volkamer lemon (Citrus volkameriana Tan & Pasq.), Carrizo citrange (Poncirus trifoliata L. Raf. X Citrus sinensis L. Osbeck) and trifoliate orange (Poncirus trifoliata Raf.) rootstocks at different NaCl concentrations. Seeds were germinated in MS medium with 0, 45, 90 and 135 mM NaCl concentrations. In general, the greatest germination rates in all salt concentrations in Volkamer lemon and sour orange rootstocks and the lowest values were observed in rough lemon and trifoliate orange rootstocks. Present findings revealed that in vitro conditions could reliably be used in salt tolerance tests of citrus rootstocks.

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