NEVAL TOPCU ALTINCI, Rustem CANGİ, Deniz ÜSTÜN

Narince Üzüm Çeşidinde Salisilik Asit Uygulamalarının Yüksek Sıcaklık Stresine Karşı Etkilerinin Belirlenmesi

Bitkiler bulundukları çevre içinde, gelişimlerini sınırlayan, farklı şekillerde meydana gelebilen olumsuz koşullara maruz kalabilmektedirler. Bitkilerde büyüme, gelişme ve metabolizmayı etkileyen ve/veya engelleyen bu olumsuz koşullara stres adı verilmektedir. Salisilik asit (SA) bitkilerde yaygın olarak bulunan ve artık günümüzde bitki büyüme ve gelişmesinin düzenlenmesinde önemli rol oynadığı kabul edilen bir fitohormondur. SA aynı zamanda, tuzluluk, yüksek ve düşük sıcaklık, su, ağır metal, don ve kuraklık stresi gibi abiyotik stres şartlarında bitkilerin toleransını artırmaktadır. Çalışmamız da Türkiye’nin önemli beyaz şaraplık üzüm çeşitlerinden olan ve Tokat’ta yaygın yetiştiriciliği yapılan Narince üzüm çeşidine ait fidanlar kullanılmıştır. Uygulanan yüksek sıcaklık stresi öncesi bitkilere 4 farklı dozda ( 0, 0.5, 1.0, 1.5 µmol l-1 ) salisilik asit (SA) uygulanmış olup bitki büyütme kabininde 40˚C’de 12 saat boyunca yüksek sıcaklığa maruz bırakılmıştır. Stres uygulamasından 21 gün sonra ise bitkilerden bitki büyümesini takip etmek amacıyla, sürgün uzunluğu (cm), yaş- kuru sürgün ve kök ağırlıkları (g) alınmış, Salisilik asidin etkisini ortaya koymak amacıyla ise oransal su kapsamı (%), iyon akışı (%) ve hücre zarı zararlanma oranı (%) hesaplanmıştır.

Determining The Effects of Salicylic Acid Applications on High Temperature Stress in Narince Grape Variety

Plants are constantly exposed to adverse conditions in their environment, which may occur in different ways, limiting their growth. These negative states that affect and / or prevent growth, development and metabolism in plants are called stress. Salicylic acid (SA) is a hormone-like substance commonly found in plants and is now accepted to play an important role in regulating plant growth and development. Salicylic acid also increases the tolerance of plants under abiotic stress conditions such as salinity, high and low temperature, water, heavy metal, frost and drought stress. This study is one of the important white wine grape varieties belonging to Turkey's Narince grape varieties growing in Tokat made widely used. Salicylic acid (SA) was applied to the plants in 4 different doses (0, 0.5, 1.0, 1.5 µmol l-1 ) before high temperature stress to be applied and they were exposed to high temperature for 12 hours at 40°C in the growth chamber. At the end of the 21 days after the stress application, shoot length (cm), fresh -dry shoot and root weights (g) were taken in order to follow the plant growth from the plants. Proportional water content (%), Ion flow (%) to reveal the effect of salicylic acid and the cell membrane damage rate (%) was calculated.

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Ağaoğlu YS. 2002. Bilimsel ve Uygulamalı Bağcılık (Asma Fizyolojisi-1). Kavaklıdere Eğitim Yayınları No. 5. 98 s. Ankara
Altıncı NT, Cangi R, Yıldız K, Yağcı A, Öztürk L, Ünlükara A. 2018. A Study on the High Temperature Treatment to Three Grapevine Varieties Grown Under in vitro Conditions. Int. J. of Agr. and Natural Sci. (IJANS) E-ISSN: 2651-3617, 1(2), 127-131
Baran A. 2011. Bitkilerin Tuz Stresine Toleransında Salisilik Asit ve Prolin’in Fizyolojik Rolünün Araştırılması Yüksek Lisans Tezi, Harran Üniv. Fen Bilimleri Ens., Biyoloji Anabilim Dalı, 55 s, Şanlıurfa
Basra AS, Cheema DS, Dhillongrewal R, Singh S, Basra RK. 1993. Proline and Polyamine Accumulation in Relation to Heat Tolerance in Tomato. In Adaptation of Food Crops to Temperature and Water Stress, 493–495.
Bertamini M, Zulini L, Muthuchelian K, Nedunchezhian N. 2006. Effect of Water Deficit on Fotosynthetic and Physiological Responses in Grapevine (Vitis Vinifea L., Cv. Riesling) Plants. Photosynthetica, 44 (1),151-154.
Camejo D, Rodríguez P, Morales MA, Dell’Amico JM, Torrecillas A, Alarcón JJ. 2005. High Temperature Effects on Photosynthetic Activity of Two Tomato Cultivars With Different Heat Susceptibility. Journal of Plant Physiology Volume 162, Issue 3, 14 March 2005, Pages 281–289. DOI:10.1016/j.jplph.2004.07.014
Chylinski WK, Lukaszewka AJ, Kutnik K. 2007. Drought Response of Two Bedding Plants. Acta Physiologiac Plantarum, 29, 399-406.
Çoban SS. 2007. Nohut Genotiplerinde Kuraklığa Bağlı Fizyolojik Parametreler ve Mineral Beslenme Üzerine Salisilik Asitin Etkisi Ankara Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi Toprak Anabilim Dalı Ankara
Dadaşoğlu E, Ekinci M. 2013. Farklı Sıcaklık Dereceleri, Tuz ve Salisilik Asit Uygulamalarının Fasulye (Phaseolus vulgaris L.) Tohumlarında Çimlenme Üzerine Etkisi. Atatürk Üniv. Ziraat Fak. Derg., 44 (2): 145-150.
Ferrini F, Mattii GB, Nicese FP. 1995. Effect of temperature on key physiological responses of grapevine leaf. Am. J. Enol. Vitic., 46: 375-379.
Greer DH, Weston C. 2010. Heat Stress Affects Flowering, Berry Growth, Sugar Accumulation and Photosynthesis of Vitis Vinifera Cv. Semillon Grapevines Grown in acontrolled Environment. Functional Plant Biology . 37(3) 206–214
Harborne JB. 1980. Plant phenolics. In: Secondary Plant Products. E. A. Bell, B. V. Charlwood (ed.), Springer Verlag, Berlin, 329-402 p.
Hasanuzzaman M, Hossain MA, da Silva JAT, Fujita M. 2012. Plant Responses and Tolerance to Abiotic Oxidative Stress: Antioxidant Defenses is a Key Factor. In Crop Stress and Its Management: Perspectives and Strategies; Bandi, V., Shanker, A.K., Shanker, C., Mandapaka, M., Eds.; Springer: Berlin, Germany, 2012; pp. 261–316.
Hasanuzzaman M, Nahar K, Fujita M. 2013. Extreme Temperatures, Oxidative Stress and Antioxidant Defense in Plants. In Abiotic Stress—Plant Responses and Applications in Agriculture; Vahdati, K., Leslie, C., Eds.; ıntech: Rijeka, Croatia, pp. 169–205.
Gregory J, Stouffer RJ, Molina M, Chidthaisong A, Solomon S, Raga G, ... & Lohmann U. 2007. Climate change 2007–The Physical Science Basis. In Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change; Cambridge University Press: Cambridge, UK, 2007.
Jackson DI, Lombard PB. 1993. Environmental and Management Practices Affecting Grape Composition and Wine Quality – A Review. Am. J.. of Enol. and Vitic., 44; 409–430.
Jones GV, White MA, Cooper OR, Storchmann K. 2005. Climate Change and Global Wine Quality. Clim Change, 73(3):319– 343.
Kara Z. 1990. Tokat Yöresinde Yetiştirilen Üzüm Çeşitlerinin Ampelografik Özelliklerinin Belirlenmesi Üzerinde Araştırmalar. Doktora Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Ankara.
Keller M. 2010. The Science of Grapevines Anatomy and Physiology. Elsevier, Amsterdam
Koca H. 2007. Tuz Stresinin Farklı Susam Çeşitlerinin Fizyolojik ve Biyokimyasal Özellikleri Üzerine Etkisi. Ege Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, 132 Sayfa, İzmir
Korkmaz A. 2005. Inclusion of Acetyl Salicylic Acid and Methyl Jasmonate into The Priming Solution Improves Low Temperature Germination and Emergence of Sweet Pepper. Hort. Sci., 40 (2005), pp. 197-200, DOI: 10.21273/HORTSCI.40.1.197
Kriedemann PE. 1968. Photosynthesis in Vine Leaves as a Function of Light İntensity, Temperature and Leaf Age. Vitis, 7: 213-220
Kuşvuran Ş. 2010. Kavunlarda Kuraklık ve Tuzluluğa Toleransın Fizyolojik Mekanizmaları Arasındaki Bağlantılar. Çukurova Üniversitesi, Fen Bilimleri Enstitüsü, Doktora Tezi, 355s. Adana.
Lebon E, Pellegrino A, Louarn G, Lecoeur J. 2006. Branch Development Controls Leaf Area Dynamics in Grapevine (Vitis Vinifera) Growing İn Drying Soil. Annals of Botany, 98: 175-185, DOI:10.1093/aob/mcl085
Lobell DB, Asner GP. 2003. Climate and Management Contributions to Recent Trends in U.S. Agricultural Yields. Science, 299:1032, DOI:10.1126/science.1078475
Lobell DB, Field CB. 2007 . Global Scale Climate–Crop Yield Relationships and The İmpacts of Recent Warming. Environ. Res. Lett. 2, DOİ:10.1088/1748-9326/2/1/014002.
Raskin I. 1992. Role of Salicylic Acid in Plants. Annu Rev Plant Physiol Plant Mol Biol 1992, 43:439-63.
Salazar-Parra C, Aguirreolea J, Sanchez-Diaz M, Irigoyen JJ. Morales F. 2010. Effects of Climate Change Scenarios on Tempranillo Grapevine (Vitis Vinifera L.) Ripening: Response to a Combination of Elevated CO2 and Temperature, and Moderate Drought. Plant Soil, 337(1-2): 179–191.
Senaratna T, Touchell D, Bunn E, Dixon K. 2000. Acetyl Salicylic Acid (Aspirin) and Salicylic Acid Induce Multiple Stress Tolerance in Bean and Tomato Plants. Plant Growth Regulation 30: 157–161,
Wahid A, Gelani S, Ashraf M, Foolad MR. 2007. Heat Tolerance in Plants: An Overview. Environ Exp Bot, 61(3):199–223.
Wang LJ, Li SH. 2006: Salicylic acid-induced heator cold tolerance in relation to Ca2+homeostasis andantioxidant systems in young grape plants. Plant Sci. 170,685–694.
Wang L, Fan L, Loescher W, Duan W, Liu G, Cheng J, Luo H, Li S. 2010. Salicylic Acid Alleviates Decreases in Photosynthesis under Heat Stress and Accelerates Recovery in Grapevine Leaves. BMC Plant Biology 2010, 10:34.
Williams LE, Dokoozlian NK, Wample R. 1994. Grape. in B. Schaffer & P.C. Andersen (Eds.), Handbook of Environmental Physiology of Fruit Crops. Vol. I. Temperate Crops (Pp. 85–133). Boca Raton, FL: CRC Press.
Winkler AJ, Cook JA, Kliewer WM, Lider LA. 1974. General Viticulture. University of California Press. Berkeley and Los Angeles. 633p.
Xingyuan Y, Fengwang M, Shuncai W, Dong L. 2004. Physiological Effects of Kiwifruit Lamina under High Temperature Stress. Journal of Northwest Sci-Tech University of Agriculture and Forestry (Natural Science Edition), 32(12):33-37.