AYFER ALKAN TORUN, HALİL ERDEM, MUSTAFA BÜLENT TORUN

Ayçiçeği Genotiplerinin Demir Noksanlığına Karşı Tolerans Düzeylerinin Belirlenmesi

Demir (Fe) noksanlığı dünyada bitkilerde ve insanlarda görülen önemli bir beslenme problemidir. Bu sorun aynı zamanda Türkiye'de de yaygın bir beslenme problemi olup ayçiçeği, önemli derecede verim ve kalite kayıplarına yol açan Fe noksanlığına duyarlı bir tür olarak bilinmektedir. Bu çalışmada farklı ayçiçeği genotiplerinin Fe noksanlığına karşı duyarlılığı test edilmiştir. Çalışmada, bitki materyali olarak, TR-6149-SA, TR-3080 ve 6480 genotipleri kullanılmıştır. Bitkiler, Fe'siz (0 µmol Fe) ve Fe (100 µmol Fe) içeren su kültürü yetiştirme ortamında test edilmiştir. Deneme sonunda bitkilerde simptom derecesi, SPAD değeri, klorofil konsantrasyonu, yeşil aksam kuru madde verimi, Fe-redüktaz enzim aktivitesi, yeşil aksam Fe konsantrasyonu ve büyüme ortamının pH değeri ölçülmüştür. Sonuçlar ayçiçeği bitkisinin Fe noksanlığını etkileyen en önemli faktörlerin Fe alımı ve Fe-redüktaz aktivitesi olduğunu göstermiştir. Çeşitlerin Fe noksanlığına karşı toleransta önemli rolü olan köklerin Fe redüktaz enzim aktivitesinin TR-3080 nolu genotipde Fe noksanlığı koşullarında diğer genotiplerden daha yüksek olduğu bulunmuştur. Sonuç olarak, uygulanan değişik Fe konsantrasyonlarına karşı genotiplerin tepkilerinin önemli derecede farklılık gösterdiği ve Fe noksanlığına karşı tolerant genotiplerin belirlenmesinde Fe redüktaz enzim aktivitesinin önemli rol oynayabileceği ortaya çıkmıştır.

Determination of Fe Deficiency Tolerance in Sunflower Genotypes

Iron deficiency (Fe) is an important nutritional disorder of plants and humans worldwide including Turkey. Sunflower is known as a Fe sensitive crop and its deficiency leads to severe yield and quality losses. In this study, the sensitivity of different sunflower genotypes to Fe deficiency was determined. For this purpose, sunflower genotypes TR6149-SA, TR-3080 and 6480 were grown either without Fe (0 µmol) or with Fe (100 µmol) hydroponically. At the end of experiment symptom grade, SPAD value, chlorophyll concentration, shoot dry matter yield, Fe-reductase activity, shoot Fe concentration and pH value of growth medium were measured. It was found out that Fe uptake and Fe reductase activity were the most important factors determining the Fedeficiency tolerance of a genotype. In the deficiency of Fe conditions, the activity of Fe reductase enzyme in the roots was found higher in the genotype of TR-3080 compared to the other genotypes. The results revealed that the genotypic responses to the different Fe concentrations applied are significantly different and Fe reductase enzyme activity may play an important role in amelioration of iron deficiency

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