Heracleum platytaenium Ekstraktlarının ve Uçucu Yağının Antioksidan Aktiviteleri

Heracleum platytaenium Boiss. geleneksel tıpta kullanılmaktadır. H. platytaenium’un uçucu yağının ve ekstrelerinin antioksidan aktivitesi incelenmiştir. Uçucu yağ, Clevenger cihazı kullanılarak su buharı damıtma yoluyla elde edildi ve bileşenler GC-MS ile belirlendi. Uçucu yağ ve ekstreler için ABTS+•, DPPH• ve indirgeme gücü aktivitesi yöntemleri kullanılarak antioksidan kapasiteleri belirlendi. Ayrıca bitkinin toplam fenolik ve toplam flavonoid içerikleri araştırıldı. n-Oktil asetat (%36,5), apiol (%24,9) ve elemisin (%20,8) bileşikleri uçucu yağın ana ürünleridir. Uçucu yağ ve ekstreler zayıf ile orta derece antioksidan aktivite sergilemişlerdir. Toplam fenolik bileşik 19,01 ile 130,99 mg GAE/g ekstre arasında ve toplam flavonoid 2,0 ile 118,4 mg QE/g ekstre arasında değişiklik göstermiştir. En yüksek DPPH serbest radikal giderme etkisi EtOAc ekstresinde gözlenmiştir (IC50 = 24,09 mg $ml-1$). EtOAc ekstresinin ABTS+ radikal giderme etkisi sentetik antioksidan olan BHA, BHT ve Trolox'dan daha yüksektir.

Antioxidant Activities of Heracleum platytaenium extracts and Essential Oil

Heracleum platytaenium Boiss. has been used in traditional medicine. Antioxidant effectof essential oil as well as extracts of H. platytaenium was investigated. The essential oilwas acquired by hydro distillation using a Clevenger type apparatus and GC-MS wasused to analyze the essential oil compounds. Antioxidant capacity including ABTS+•,DPPH• scavenging and reducing power activity tests were carried out for essential oil andextracts. Moreover, total phenolic and total flavonoid contents were investigated. n-Octylacetate (36.5%), apiole (24.9%), and elemicin (20.8%) were the chief products ofessential oil. The essential oil and extracts exhibited from weak to moderate activity. Thetotal phenol varied from 19.01 to 130.99 mg GAE/g extract and total flavonoid wasfluctuated from 2.0 to 118.4 mg QE/g extract. The most $DPPH•$ scavenging effect wasobserved in EtOAc extract (IC50 = 24.09 mg ml $–1$). The $ABTS•+$ scavenging effect ofEtOAc extract was better than synthetic antioxidants BHA, BHT and Trolox.

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