Likenler ve moleküller biyoloji uygulamaları

Polimeraz zincir reaksiyonu (PZR), liken filogenisi ve populasyon genetiğinin moleküler incelemelerinin yapılabilmesi için oldukça basit ve güçlü bir tekniktir. Likenler alg ve mantardan oluşan simbiyotik bir birlikteliktir bu nedenle moleküler çalışmalarda özel dikkat edilmesi gerekir. Bu derlemede, likenlerden DNA ekstraksiyonu, RAPD metodu ve rRNA'nın ITS bölgesi çalışmaları üzerine yoğunlaşılmıştır. Önümüzdeki yıllarda, moleküler biyolojik teknikler daha güvenli tanımlamalar için kullanılabilecek ve yeni moleküler teknikler liken filogenisini daha iyi anlayabilmemize katkıda bulunacaktır.

Lichens and molecular biological applications

The Polymerase Chain Reaction (PCR) is a fairly simple but powerful technique for molecular investigations of lichen phylogeny and population genetics. Lichens are a symbiotic association of a fungus and alga, thus they require special considerations for molecular studies. The present review focuses on nucleic acid extraction from lichens studies on randomly amplified polymorphic DNA (RAPD) method and the internal transcribed spacer regions of rRNA. In the coming years, molecular biological techniques will be used for more accurate identification of lichen and the newly available molecular methods will significantly contribute to our understanding of lichen phylogeny.

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