Burak ÇİTİL, Yasemin BAYRAM, Selçuk KILIÇ, Bekir ÇELEBİ

Francisella tularensis antikorları ile Brucella çapraz reaksiyonlarının araştırılması

Amaç: Francisella tularensis antikorları ile Brucella, Legionella, Yersinia ve Mycoplasma türleri arasında çapraz reaksiyonlar bildirilmiştir.F.tularensis liposakkarit yapısında bulunanBrucellaO-polisakkaritindeki benzer antijenik yapılar serolojiktestlerde çapraz reaksiyonlara neden olabilmektedir.Aglütinasyon testlerinde F.tularensis ve Brucella abortus arasındaki çapraz reaksiyonlar sonuçlarınyorumlanmasını güçleştirebilir.Yöntem: Brucella ve F.tularensis arasındaki çapraz reaksiyonların sıklığı ve bu reaksiyonlardan sorumluolan antikorlar mikroaglütinasyon, Brucella ELISAve 2-merkaptoetanol mikroaglütinasyon testleri ilearaştırılmıştır.Bulgular: 260 tularemi pozitif örneğin 49 (%18.8)’unda B.abortus antijeniyle çapraz reaksiyonlar saptanırken, bruselloz pozitif 252 örneğin 23(%9.1)’ünde F.tularensis antijeni ile çapraz reaksiyongözlenmiştir. Otuzaltı tularemi (%73.5) pozitif serumdaBrucella çapraz reaksiyon titreleri 1:20-1:80 iken, 13örnekte (%26.5) ise ≥1:160 titrelerde çapraz reaksiyongözlenmiştir. 2-ME testi ile

Investigation of cross-reactions with Francisella tularensis antibodies to Brucella

Objective: Cross-reactions with Francisella tularensis antibodies to Brucella, Legionella, Yersinia, and Mycoplasma species have been described. F.tularensis LPS is able to induce serological cross- reactions indistinguishable from brucellosis due to a similar immunodominant epitope in the Brucella O-polysaccharide. In agglutination tests, cross-reaction occurred only between F.tularensis and Brucella abortus, cross reactivity with Brucella can confuse interpretation of the results. Method: The aim of this study was to assess cross reactions and whether IgM or IgG antibodies are responsible for Brucella and F.tularensis cross- reactions, by the use of microagglutination,Brucella ELISA and 2-mercaptoethanol microagglutination test Results: Cross-reaction agglutinin titers to B.abortus antigen were found in 49 of 260 (18.8%) tularemia positive serum specimens, and cross-reaction titers to F.tularensis antigen were found in 23 of 252 (9.1%) Brucella positive serum specimens. While thirty six (73.5%) tularemia positive serum titers had Brucella cross reactivity at the titers of 1:20-1:80, cross-reaction with ≥1:160 titers was found in 13 samples (26.5%). The cross-reaction titers to B.abortus antigen were reduced to equal or less 1:40 titers by 2-ME. Cross- reactivity at the titers of 1:20-1:80 was determined in 22 of 23 Brucella positive samples. Conclusion: The cross-reaction titers determined with MA test in both infections were reduced to 40 or less by 2-ME, suggesting that the titers are due to immunoglobulin M antibody.

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