Aysel KIZILTAY, Zeynep GÜNDOĞAN, İrem Erel GÖKTEPE, Nesrin HASIRCI

ceftriaxone sodyumun kontrollü salımı için çok katmanlı polimerik filmler

Amaç: Bu çalışmanın amacı konakçı bölgede enfeksiyonu tedavi etmek amacıyla medikal alanda kullanılabilecek kontrollü salım sistemi hazırlamaktır.Yöntem: Mikro katmanlı filmler çözelti döküm metodu ile hazırlanmıştır. Üç katmanlı filmler kitosan CHI , jelatin GEL ve aljinat ALG kullanılarak aljinat tabakasına ceftriaxone sodyum CS yüklenmiş, ayrıca kontrol grubu için ilaç yüklenmemiş olarak hazırlanmış ve sırasıyla CHI /ALG/CHI ve CHI-GEL/ALG-CS/CHI-GEL olarak kodlandırılmıştır. Bütün filmler glutaraldehit GA buharına farklı süre boyunca 2 h, 10 h veya 24 h tabi tutularak çapraz bağlanmıştır. Filmlerin mekanik özellikleri ve CS’nin filmlerden salım kinetikleri üç farklı pH şartlarında pH 5,5, 7,4, ve 10,0 incelenmiştir. Salınan CS’nin Escherichia coli’ye karşı antibakteriyel etkinliği agar spot yöntemiyle test edilmiştir. Bulgular: Sonuçlar üç-katmanlı film yapılarının üst ve alt katmanında GEL varlığının filmlerin kırılganlığı önlediğini ve mekanik dayanımını arttırdığını, ancak orta katmanda CS varlığının mekanik özellikleri düşürdüğünü göstermiştir. Filmlerin GA ile çapraz bağlanması CS’nin salımında anlamlı bir etki göstermemiştir, ancak kitosanın aljinat ve CS ile etkileşiminden dolayı ilaç salım hızında azalma olmuştur. İlaç yüklü filmlerden salınan ilaç çözeltisinin kullanılmasıyla E. coli ile yapılan antibakteriyel deneyler, agar spor testlerinde inhibisyon zonu oluştuğunu göstermiştir. Sonuç: Çözücü döküm metodu ile hazırlanan çok katmanlı filmlerin medikal uygulamalarda ilaç taşıyıcı sistem olarak kullanım için iyi bir aday olduğu düşünülmektedir

Anahtar Kelimeler:

Jelatin, kitosan, aljinat, ceftriaxone sodyum, polimerik film, ilaç salımı

Multilayer polymeric films for controlled release of ceftriaxone sodium

Objective: The objective of this study was to prepare a controlled release system, which could be used in medical applications in order to treat infections at the host region.Methods: Microlayer films were prepared via solvent casting method. Films with 3-layers were prepared by using chitosan CHI , gelatin GEL and alginate ALG in the form of CHI /ALG/CHI and CHI-GEL /ALG/CHI-GEL with or without ceftriaxone sodium CS which is loaded in the middle ALG layer. All films were crosslinked by exposing them to glutaraldehyde GA vapor for different 2 h, 10 h or 24 h durations. Mechanical properties of the films and release kinetics of CS at three different pH conditions pH 5.5, 7.4, and 10.0 were investigated. The antibacterial efficiency of the released CS against Escherichia coli was examined via agar spot test.Results: The results indicated that the presence of GEL in the upper and lower layers of the 3-layer construct prevented fragility and increased the mechanical strength of the films, whereas the presence of CS in the middle layer caused decrease in the mechanical properties. Crosslinking with GA did not demonstrate a significant effect on the release profile of CS, but salım hızında azalma olmuştur. İlaç yüklü filmlerden salınan ilaç çözeltisinin kullanılmasıyla E. coli ile yapılan antibakteriyel deneyler, agar spor testlerinde inhibisyon zonu oluştuğunu göstermiştir. Sonuç: Çözücü döküm metodu ile hazırlanan çok katmanlı filmlerin medikal uygulamalarda ilaç taşıyıcı sistem olarak kullanım için iyi bir aday olduğu düşünülmektedir

Keywords:

Gelatin, chitosan, alginate, ceftriaxone sodium, polymeric film, drug release,

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