Hasan ESEN, Sami CERAN, Güven SUNAM, Mustafa GÜLTEKİN, Bayram ALTUNTAŞ

Tavşanlarda ameliyat öncesi kemoterapi ve beta glukanın bronş anastomozuna etkileri: Preliminar bir çalışma

Amaç: Bu çalışmada ameliyat öncesi dönemde kemoterapigören tavşanlarda bir immünstimülan olan beta glukanınbronş anastomozuna etkileri araştırıldı. Çalışmaplanı:Bu çalışmada kırk beş Yeni Zelanda türüerkek tavşan kullanıldı. Tavşanlar her birinde 15 tavşanolacak şekilde grup 1 (kontrol), grup 2 (kemoterapi)ve grup 3 (kemoterapi + beta glukan) olarak üç grubabölündü. Tüm gruplarda sol ana bronş tam olarak kesildi vedaha sonra uç uca anastomoz yapıldı. Grup 2 de ameliyatöncesi birinci günde sisplatin ve ameliyat öncesi birinci,ikinci ve üçüncü günde etoposid uygulandı. Grup 3 te aynıkemoterapi protokolüne ilave olarak ameliyattan yedi günönceden başlanarak sakrifikasyonların bir gün öncesinekadar enteral yolla 10 mg/kg/gün beta glukan verildi.Tavşanlar ameliyat sonrası üçüncü, beşinci ve yedincigünlerde beşerli gruplar halinde sakrifiye edildi. Bronşiyalanastomoz hatları çıkarıldı ve doku kesitleri hematoksilinve eozin ile boyandı. Bul gu lar: Patolojik incelemede grup 1 ve grup 2 arasındave grup 2 ve grup 3 arasında istatistiksel olarak anlamlıfarklılık var iken grup 1 ve grup 3 arasında bronşiyileşmesi açısından farklılık yok idi. So nuç: Kemoterapi tedavisi sırasında beta glukankullanılması ameliyat sonrası dönemde bronşiyal iyileşmeyidüzeltebilir.

The effects of preoperative chemotherapy and beta glucan on bronchial anastomosis in rabbits: a preliminary study

Background: This study aims to investigate the effects of animmunostimulant, beta glucan, on the bronchial anastomosisin rabbits that received chemotherapy in preoperative period. Methods: Forty-five New Zealand male rabbits were usedin this study. The rabbits were divided into three groupsas group 1 (control), group 2 (chemotherapy), and group 3(chemotherapy + beta glucan) with 15 rabbits in each group.The left main bronchus was incised completely and then end- to-end anastomosis was performed in all groups. Cisplatin wasadministered on the preoperative first day and etoposide wasadministered on the preoperative first, second, and third daysin group 2. In group 3, in addition to the same chemotherapyprotocol, 10 mg/kg/day beta glucan was administered viaenteral route starting from seven days before operation untilone day before sacrifications. The rabbits were sacrificed atpostoperative third, fifth, and seventh days by groups of five.The bronchial anastomosis lines were removed and tissuesections were stained with hematoxylin and eosin. Results: In pathological analysis, while there were statisticallysignificant differences between group 1 and group 2 andbetween group 2 and group 3, there was no differencebetween group 1 and group 3 in terms of bronchial healing. Conclusion: The use of beta glucan during chemotherapytreatment may improve bronchial healing at postoperativeperiod.

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