Nermin KARAHAN, İlker KİRİŞ, Şahin KAPAN, Hüseyin OKUTAN, Aynur KILBAŞ, İrfan ALTUNTAŞ

Eritropoietinin sıçan aortik iskemi-reperfüzyonunda akciğer hasarı üzerine etkisi

Amaç: Bu çalışmada sıçan infrarenal abdominal aortunda(İAA) oklüzyon-reperfüzyon sonrası akciğerlerde oluşaniskemi-reperfüzyon (İR) hasarına eritropoietinin etkisiaraştırıldı. Ça lış ma pla nı: Yirmi dört adet Wistar albino cinsi sıçanrastgele ve eşit sayıda üç gruba ayrıldı. Kontrol grubundalaparatomi ve İAA diseksiyonu yapıldı, İR uygulanmadı.Aortik İR grubunda, İAA’ya travmatik olmayan mik- rovasküler klemp konularak 30 dakika iskemi, klempkaldırılarak 60 dakika reperfüzyon uygulandı. AortikİR+eritropoietin grubunda da 30 dakika iskemi, 60 daki- ka reperfüzyon uygulandı ve ayrıca klemp kaldırılmadan5 dakika önce 1000 Ü/kg eritropoietin subkutan yollaverildi. Anestezi altında yaşamları sonlandırılan sıçanlarınakciğerleri çıkarıldı ve akciğer dokularında malondialde- hid (MDA) düzeyi ve süperoksit dismutaz (SOD), katalazve glutatyon peroksidaz aktivite düzeyleri ölçüldü ve his- topatolojik inceleme yapıldı. Bul gu lar: Aortik İR grubunda MDA, SOD ve katalazdeğerleri kontrol grubundaki değerlerden anlamlı dere- cede yüksek bulundu (p

The effect of erythropoietin on lung injury in rat aortic ischemia-reperfusion

Background: We investigated the effect of erythropoietinon ischemia-reperfusion (IR) injury in lungs occurringafter occlusion–reperfusion of rat infrarenal abdominalaorta (IAA). Methods: Twenty-four Wistar albino rats were randomizedto three groups equal in number. Control rats underwentlaparotomy and IAA dissection without IR. Two groupsunderwent aortic IR designed as 30 min of ischemia byclamping of the IAA with an atraumatic microvascularclamp, followed by 60 min of reperfusion after removal ofthe aortic clamp. In one aortic IR group, 1000 U/kg eryth- ropoietin was administered subcutaneously 5 min beforedeclamping of the IAA. The rats were decapitated underdeep anesthesia and the lungs were removed. Tissue levelsof malondialdehyde (MDA) and activity levels of superox- ide dismutase (SOD), catalase, and glutathione peroxidasewere measured and lung specimens were examined histo- pathologically. Results: Rats treated with aortic IR alone had significantlyhigher tissue MDA levels and activity levels of SOD and cata- lase compared to the control group (p

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