Gamze GÖGER, Esma ÇEVİK, Alişan VARNALI, Ömer Koray YAYLACI, Medine Münevver UMA, Gülmira ÖZEK

SCREENING OF Achillea coarctata Poir. AND Achillea setacea Waldst. & Kit. (Asteraceae) FOR THEIR VOLATILE AND FATTY ACIDS COMPOSITIONS, AND ANTIMICROBIAL ACTIVITIES

Bu çalışmanın ana amacı, Achillea setacea Waldst. & Kit. ve Achillea coarctata Poir. ‘nın toprak üstü kısımlarından elde edilen n-hekzan, etil asetat ve sulu ekstrelerinin Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 14028 ve Candida albicans ATCC 6633'e karşı mikrodilüsyon yöntemi ile antimikrobiyal aktivitesinin belirlenmesidir. Buna ek olarak, A. coarctata'nın flukonazol içeren sulu ekstresinin C. albicans ATCC 6633, C. parapsilosis ATCC 22019, C. tropicalis ATCC 750 ve C. glabrata ATCC 90030'a karşı etkisi dama tahtası yöntemiyle araştırılmıştır. Uçucu bileşenlerin ve yağ asidi metil esterlerinin kimyasal bileşimleri Gaz Kromatografisi (GK) ve Gaz Kromatografisi/Kütle Spektrometresi (GK/KS) ile tanımlanmıştır. Achillae setacea'nın uçucu yağının ana bileşenleri 1,8-sineol (%16,1) ve β-pinen (%14,5), A. coarctata’nın ise kafur (%35,4) ve borneol (%12,9) olarak belirlenmiştir. Achillae setacea ve A. coarctata'nın etil asetat ekstrelerinin (MİK=156,25 µg/mL ve MİK=312,5 µg/mL, sırasıyla) test edilen Candida türlerine karşı daha etkili olduğu bulunmuştur. Achillae coarctata su ekstresinin flukonazol ile kombinasyonu test edilen Candida Berkh. türleri için FIC= 0,53-0,625 µg/mL arasında aditif etki göstermiştir.

SCREENING OF Achillea coarctata Poir. AND Achillea setacea Waldst. & Kit. (Asteraceae) FOR THEIR VOLATILE AND FATTY ACIDS COMPOSITIONS, AND ANTIMICROBIAL ACTIVITIES

The main aim of this study is antimicrobial screening of the n-hexane, ethyl acetate and aqueous extracts obtained from aerial parts of Achillea setacea Waldst. & Kit. and Achillea coarctata Poir. against the bacterial strains Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 14028 and the yeast Candida albicans ATCC 6633 by the microdilution method. In addition, the effect of the aqueous extract of A. coarctata combined with fluconazole againts C. albicans ATCC 6633, C. parapsilosis ATCC 22019, C. tropicalis ATCC 750 and C. glabrata ATCC 90030 was tested with the checkerboard method. The chemical components of the volatiles and fatty acid methyl esters were identified using the Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS) techniques. The main components of the volatile oil were found to be 1,8-cineole (16.1%) and β-pinene (14.5%) for A. setacea and camphor (35.4%) and borneol (12.9%) for A. coarctata. The ethyl acetate extracts of A. setacea (MIC=156.25 µg/mL) and A. coarctata (MIC=312.5 µg/mL) were found more active against the tested Candida Berkh. species. The combination of aqueous extract of A. coarctata with fluconazole showed additive effect for the tested Candida species with FIC values within the range of 0.53-0.625 µg/mL.

Keywords:

Achillea setacea, Achillea coarctata, Phytochemical analyses, GC/MS, Checkerboard, Additive effect,

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