Delastria, A NEW GENUS OF HYPOGEOUS FUNGI RECORD FOR THE TURKISH MYCOBIOTA
Bu çalışmada 2022 yılında, Edirne'nin Enez ve Süloğlu ilçerinden trüf dedektör köpeklerin yardımıyla toplanan hipogean fungal örnekler analiz edilmiştir. Örneklerin makroskobik özellikleri ve araştırma alanının ekolojik detayları doğal yaşam alanlarında belgelenmiştir. Örnekler ışık ve taramalı elektron mikroskobuyla araştırılmış, ve aynı zamanda ITS rDNA temelli moleküler filogenetik analiz edilerek Delastria rosea Tul. & C. Tul.'a ait oldukları tespit edilmiştir. Bu tanı, morfolojik verilerle de desteklenmiştir. Bu kayıt Türkiye'den D. rosea'nın cins ve tür düzeyinde ilk kaydıdır. Yeni kaydedilen türün kısa bir tanımı verilmiştir. Sporların ışık ve taramalı elektron mikroskobu (SEM) ile çekilen fotoğrafları dahil edilmiştir. Bu çalışma ile Türkiye trüf ve tüf benzeri mantarların cins ve tür sayısı sırasıyla 36 ve 105'e yükselmiştir.
Delastria, A NEW GENUS OF HYPOGEOUS FUNGI RECORD FOR THE TURKISH MYCOBIOTA
In the present study, fungal samples collected from Enez and Süloğlu districts of Edirne Province, in Türkiye with the help of truffle-detecting dogs were analyzed. The macroscopic features and environmental details of the collection sites were documented in their natural habitats. The samples were investigated with light- and scanning electron microscopy (SEM) and were also used in ITS rDNA-based molecular phylogenetic analysis, which revealed that they belong to Delastria rosea Tul. & C. Tul. The identification was further supported by morphological data. This is the first record of D. rosea in Türkiye at the genus and species level. A brief description of the newly reported species is provided. Macro- and microphotographs of the spores taken by both ligth and electron microscop (SEM). With this study, the number of genera and species of Turkish truffles and truffle-like fungi has increased to 36 and 105, respectively.
___
- 1. Akata, I., Şen, İ., Sevindik, M. & Kabaktepe, Ş. 2022. Truffle checklist of Turkey II with a new record. Turkish Journal of Agriculture-Food Science and Technology, 10(10): 1913-1920. https://doi.org/10.24925/turjaf.v10i10.1913-1920.5482
- 2. Alvarado, P., Moreno, G., Manjón, J.L., Gelpi, C., Kaounas, V., Konstantinidis, G., Barseghyan G.S. & Venturella G. 2011. First molecular data on Delastria rosea, Fischerula macrospora, and Hydnocystis piligera. Boletín de la Sociedad Micológica de Madrid, 35: 31-37.
- 3. Castellano, M.A., Trappe, J.M. & Luoma, D.L. 2004. Sequestrate fungi. pp. 197-213. In: (Mueller, G.M., Bills, G.F. & Foster, M.S. (eds.). Biodiversity of fungi. inventory and monitoring methods. Elsevier, Amsterdam, 777 pp.
- 4. Clark, K., Karsch-Mizrachi, I., Lipman, D. J., Ostell, J. & Sayers, E.W. 2016. GenBank. Nucleic Acids Research, 44(D1): D67-D72.
- 5. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 39(4): 783-791.
- 6. Gilkey H.M. 1954. Tuberales, North American Flora. series II, Part I. The New York Botanical Garden, New York, 36 pp.
- 7. Gómez-Reyes, V.M., Gómez-Peralta, M. & Guevara Guerrero, G. 2017. Primer reporte y distribución de Delastria rosea (Pezizales: Incertae sedis) en México. Acta botánica mexicana, 119: 139-144. https://doi.org/10.21829/abm119.2017.1236
- 8. Harkness H.W. 1899. Californian hypogæous Fungi. Proceedings of the California Academy of Sciences, Botany, 3(8): 241-293.
- 9. Henkrar, F., Meyad, C., Sabaa, S & Khabar, L. 2022. Desert truffles and truffles in morocco: biodiversity of promising fungi to combat desertification. Environmental Sciences Proceedings, 16(1): 35. https://doi.org/10.3390/environsciproc2022016035
- 10. Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular biology and evolution, 35(6): 1547-1549. https://doi.org/10.1093/molbev/msy096
- 11. Henkrar, F. & Khabar, L. 2022. Proximate analysis of lipid composition in Moroccan truffles and desert truffles. Italian Journal of Food Science, 34(2): 67-73. https://doi.org/10.15586/ijfs.v34i2.2202
- 12. Parks H.E. 1921. California Hypogaeous Fungi -Tuberaceae. Mycologia, 13(6): 301-314.
- 13. Paz A. & Lavoise, C. 2013. Propuesta de dos nuevas especies de hongos hipogeos y una primera cita para la Peninsula Ibérica. Boletín Micológico de la FAMCAL, 8: 71-86.
- 14. Paz, A., Lavoise, C., Chautrand, P., Moreau, P. A. & Bellanger, J. M. 2018. The genus Delastria (Pezizaceae), a worldwide revision. Ascomycete.org, 10(6): 229-243. https://doi.org/10.25664/ART-0247
- 15. Raja, H. A., Miller, A. N., Pearce, C. J. & Oberlies, N. H. 2017. Fungal identification using molecular tools: a primer for the natural products research community. Journal of natural products, 80(3): 756-770. https://doi.org/10.1021/acs.jnatprod.6b01085
- 16. Rogers, S. O. & Bendich, A. J. 1994. Extraction of total cellular DNA from plants, algae and fungi. pp. 183-190. In: Gelvin, S.B. & Schilperoort, R.A. (eds). Plant molecular biology manual. Springer Netherlands, Dordrecht, 598 pp.
- 17. Sesli, E., Asan, A., Selçuk, F., Arabacı Günyar, Ö., Akata, I., Akgül, H., Aktaş, S., Alkan, S., Allı, H., Aydoğdu, H., Berikten, D., Demirel, K., Demirel, R., Doğan, H.H., Erdoğdu, M., Ergül, C.C., Eroğlu, G., Giray, G.., Halik Uztan, A., Kabaktepe, Ş., Kadaifçiler, D., Kalyoncu, F., Karaltı, İ., Kaşık, G., Kaya, A., Keleş, A., Kırbağ, S., Kıvanç, M., Ocak, İ., Ökten, S., Özkale, E., Öztürk, C., Sevindik, M., Şen, B., Şen, İ., Türkekul, İ., Ulukapı, M., Uzun, Ya., Uzun, Yu. & Yoltaş, A. 2020. Türkiye Mantarları Listesi. Ali Nihat Gökyiğit Vakfı Yayını, İstanbul, 1177 pp.
- 18. Stielow, J. B. , Lévesque, C. A. , Seifert, K. A. , et al. 2015. One fungus, which genes? Development and assessment of universal primers for potential secondary fungal DNA barcodes. Persoonia-Molecular Phylogeny and Evolution of Fungi, 35(1): 242-263. https://doi.org/10.3767/003158515X689135
- 19. Tamura, K. 1992. Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9: 678-687.
- 20. Tulasne, L.R & Tulasne, C. 1843. Champignons hypogés de la famille des Lycoperdaceae. Annales des sciences naturelles, Botanique, série 19(2): 373-381.