Kemal Murat OKÇU, NECDET DOĞAN, Metin ŞENÇİMEN, Cem KORKMAZ, HASAN AYBERK ALTUĞ, Tayfun İDE, MESUT AKYOL

The effect of a hemostatic agent (fastact) to wound and tissue repair in a rat model

Amaç: Bu çalışmanın amacı, in vivo olarak hemostatik bir ajanın (FastAct) prilokain hidroklorid ile beraber veya onsuz, yara ve doku iyileşmesini kolaylaştırdığı hipotezinin test edilmesidir. Yara iyileşmesi kanama ve hemostaz ile başlayan kompleks bir prosedürdür. Sığır protein faktörleri II, VII, IX ve X'u ihtiva eden FastAct pıhtı oluşumunda faktör II, V, VIII ve XIII'ü aktive etmesi için katalizör olarak kullanılır. Gereçler ve Yöntemler: Çalışma 21 erkek Wistar rat üzerinde gerçekleştirildi. Tüm hayvanların sırtında 4 cm'lik kutanöz insizyonlar yapıldı. Yedi hayvanda yara kenarları arasına sadece FastAct uygulanırken, diğer yedisinde FastAct prilokain ile birlikte uygulandı. Yara dudakları rezorbe olan sutur materyali ile kapatıldı. Bulgular: Örnekler yedinci gün elde edildi. Histolojik inceleme öncesinde örneklere germe ve çekme testleri uygulandı. FastAct grubunda epitelizasyon ve yara iyileşmesinin diğer gruba göre daha iyi olduğu tespit edildi. Germe ve çekme testlerinde FastAct grubu sonuçlarının daha iyi olduğu gözlendi. Sonuç: FastAct'in hemostazisi sağladığı, doğal süreçler yardımıyla yara iyileşmesini kolaylaştırdığı sonucuna ulaşılmıştır.

Bir hemostatik ajanin (fastact) deneysel rat modelinde yara ve doku iyileşmesi üzerine etkisi

Objectives: The purpose of this study was to test the hypothesis that the hemostatic agent (FastAct) exerts favourable effect on wound healing and tissue repair alone and along with prilocaine hydrochloride in vivo. Wound healing process is a complex procedure that starts with bleeding and hemostasis. Comprising of bovine factor proteins II, VII, IX and X, FastAct is used as a catalyst in the clot formation process to activate factors II, V, VIII and XIII. Materials and Methods: The study was performed on 21 Wistar male rats. Cutaneous incisions at the length of 4 cm. were made on the dorsal side of all animals. In seven, FastAct was applied alone between the wound edges and, in the other seven, FastAct was applied along with prilocaine. The wounds were sutured by resorbable suture material. Results: The specimens were obtained on the seventh day. Wound breaking strength test was performed before histological examination. In the FastAct group the epithelialization and the closure of the wound edge were better than the other groups. The results of the breaking strength test showed that the results were best in FastAct group. Conclusion: It's concluded that FastAct accomplished hemostasis, facilitated wound healing in accordance with natural processes.

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