The cytogenetic effects of maleic hydrazide in human lymphocyte culture

Bu çalışmada Maleik Hidrazid'in (MH) farklı konsantrasyon ve muamele sürelerinde insan lenfositleri üzerindeki sitogenetik etkisi periferal kan kültürü yöntemiyle araştırılmıştır. Bu amaçla distile suda eritilen MH'in 75, 300, 500 ve 1000 μg/ml'lik konsantrasyonları kullanılmıştır. İnsan lenfositleri farklı konsantrasyonlardaki MH ile 24 ve 48 saat süre boyunca muamele edilmiştir. Kromatid gap, kromatid kırık, izokromatid gap ve isokromatid kırık gibi kromozom anormallikleri meydana geldiği saptandı. Sonuçlar iki şekilde değerlendirilmiştir. Gaplar kromozom aberasyonları (CA) olarak kabul edildiğinde 75 μg/ml MH'in 48 saat muamele edilmiş grup dışındaki tüm gruplarda MH insan lenfositlerinde kontrole göre anlamlı CA'larına sebep olduğu görülmüştür. Gaplar CA olarak kabul edilmediğinde MH'in insan lenfositleri üzerine etkili olmadığı saptanmıştır. MH artan konsantrasyon ve muamele süresine bağlı olarak mitotik indeksi (MI) de azaltmıştır.

Anahtar Kelimeler:

kromozom hataları, maleik hidrazit, lenfositler, büyüme engelleyiciler, sitogenetik, hücre kültürü, insan

Maleik Hidrazid'in İnsan lenfosit kültürüne sitogenetik etkileri

In this study the cytogenetic effects of Maleic hydrazide (MH) at different concentration and treatment periods were investigated in peripheral blood culture. For this purpose, 75, 300, 500, 1000 μg/ml concentrations of MH dissolved in distilled water were used. Human lymphocytes were treated with MH at different concentrations for 24 and 48 hours. Chromosome abnormalities were observed such as chromatid gap, chromatid break, isochromatid gap and isochromatid break. Results were evaluated in two ways. If gaps are accepted as chromosome aberrations (CA), at all the groups MH significantly induced CA except 75 μg/ml 48 hour treatment group compared with control. If not, it was observed that MH is not affective. MH also decreased mitotic index (MI) depending upon the increasing concentrations and treatment periods.

Keywords:

chromosome aberrations, maleic hydrazide, lymphocytes, growth inhibitors, cytogenetics, cell culture, man,

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