Ceren GÜNEL, Buket DEMİRCİ, İbrahim METEOĞLU, Mustafa YILMAZ, İmran KURT ÖMÜRLÜ, Tolga KOCATÜRK

Sıçan alerjik rinit modelinde timokinonun anti-enflamatuvar etkileri

Amaç: Bu çalışmada sıçan alerjik rinit AR modelinde timokinonun TQ havayolu enflamasyonu üzerindeki etkisi araştırıldı.Gereç ve Yöntem: Ovalbümin OVA ile intraperitoneal sensitizasyon ve intranazal uygulama ile 42 sıçanda alerjik rinit indüklendi. Hayvanlar altı alt gruba ayrıldı kafes başına n=7 : sağlıklı kontroller, AR grubu, kortikosteroid ile tedavi edilen AR grubu 1 mg/kg deksametazon; CS+AR , sadece 10 mg/kg TQ verilen sağlıklı sıçanlar TQ10 , 3 mg/kg TQ ile tedavi edilen AR grubu TQ3+AR ve 10 mg/kg TQ ile tedavi edilen AR grubu TQ10+AR . İnterferon-gama IFN-g , interlökin-4 IL-4 , IL-10 ve OVA’ya özgü immünoglobülin E’nin Ig-E serum düzeyleri ölçüldü. Nazal mukozadaki histopatolojik değişiklikler ve tümör nekroz faktör-alfa TNF-a ve IL-1b ekspresyonu değerlendirildi.Bulgular: Timokinon, IL-4, OVA’ya özgü IgE ve TNF-a ve IL-1b’nın ekspresyonunu engelledi. Aynı zamanda burun mukozasında eozinofil infiltrasyonunu ve ödemi azalttı, ancak IFN-g ve IL-10 üzerinde artmış bir etki göstermedi.Sonuç: Timokinonun alerjik reaksiyon üzerinde birden fazla baskılayıcı etkiye sahip olduğu gözlemlendi. Timokinon, alerjik rinit tedavisinde yardımcı bir ajan olarak düşünülebilir

Anahtar Kelimeler:

İnterlökin-10, interlökin-1 beta, interlökin-4, ovalbümine özgü IgE, timokinon, alerjik rinit, tümör nekroz faktör-alfa

The anti-inflammatory effects of thymoquinone in a rat model of allergic rhinitis

Objectives: This study aims to investigate the effect of thymoquinone TQ on airway inflammation in a rat model of allergic rhinitis AR . Materials and Methods: Allergic rhinitis was induced in 42 rats by intraperitoneal sensitization and intranasal challenge with ovalbumin OVA . The animals were divided into six subgroups n=7/per cage : healthy controls, AR group, AR group treated with corticosteroid dexamethasone 1 mg/kg; CS+AR , healthy rats that were given only TQ of 10 mg/kg TQ10 , AR group treated with TQ of 3 mg/kg TQ3+AR and AR group treated with TQ of 10 mg/kg TQ10+AR . We measured the serum levels of interferon-gamma IFN-g , interleukin-4 IL-4 , IL-10, and OVA-specific immunoglobulin E Ig-E . Histopathologic changes in nasal mucosa and expression of tumor necrosis factor-alpha TNF-a and IL-1b were evaluated. Results: Thymoquinone has inhibited the production of the IL-4, OVA-specific IgE, and the expression of TNF-a and IL-1b. It also reduced eosinophil infiltration and edema in the nasal mucosa while it has no increased effect on IFN-g and IL-10. Conclusion: We observed that TQ has multiple suppressive effects on allergic response. Thymoquinone may be considered as a supplemental agent in the treatment of allergic rhinitis.

Keywords:

Interleukin-10, interleukin-1 beta, interleukin-4, ovalbumin-specific IgE, thymoquinone, allergic rhinitis, tumor necrosis factor-alpha,

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