Dyestuffs are used in almost every industry. However, due to the negative effects of synthetic dyestuffs on the environment and human health, environmentally friendly natural dyestuffs have gained importance in recent years, especially in the textile sector. Mostly plant and plant waste-based dyes are used in coloring fabrics with natural dyes. However, with the developments in the field of biotechnology, there are also studies in which pigment is produced by microorganisms. Within the scope of this study, 100% cotton woven fabric was dyed using 6 different processes with 3 bio-colors, pink, blue and brown, produced by bacterial fermentation. The dyeings were carried out in the absence of chemicals, in the presence of salt, salt-soda, salt-alum, salt-soda-alum and alum respectively. In order to evaluate the dyeing performance, the CIELab values of the fabrics were measured and their color fastness to washing, water, alkali and acid perspiration and also dry and wet rubbing fastness were checked. As a result of the studies, it was concluded that each dyestuff react in a different way with a different process. While the most suitable process for blue and pink bio-color is that salt used process, the highest K/S value with brown bio-color was obtained as a result of dyeing with salt-soda. All fastness values of the dyed fabrics were quite good. As a result of the trials, it can be said that laboratory-scale studies of bio-colors obtained by bacterial fermentation are promising for the future.