Murat ARDIÇ, Onur KOYUNCU, Güleray AĞAR, Metin TURAN, Zekiye BAŞÇİFTÇİ BUDAK, N. Gözde AYTER, Murat OLGUN, Engin TAKIL

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Identification of Genetic Divergence in Some Bread Wheat Varieties By Rapd and Issr Analyses

Bu çalışmada, bazı buğday çeşitlerinin arasındaki RAPD ve ISSR analizine dayalı genetik mesafelerin belirlenerek ayrımının yapılması amaçlanmıştır. Araştırma sonuçlarına göre, primerler genotipleri güçlendirilmiş bantların sayısı RAPD de 3-10, ISSR de 6-10 arasında değiştiği belirlenmiş olup, primer/primer kombinasyonları içerisinde RAPDde OPBA-03, OPL-15 ve OPY- 13den; ISSRde UBC810 elde edildiği ortaya konmuştur. Dendogram sonuçlarına göre çeşitler göre üç grup altında sınıflandırılmıştır. Yunus, Altay-2000, Naci Bey, Sultan-95, Harmankaya ve Soyer çeşitlerinin uzak ilişkili; Alpu-01 ve Nacibey çeşitlerinin ise yakın ilişkili çeşitler olduğu tespit edilmiştir. Yine RAPD ve ISSR yöntemlerinin genetik varyasyonu değerlendirmek, yeni çeşitlerin geliştirilmesi ve sınıflamasında kullanılabilir yöntemler olduğu belirlenmiştir.

Bazı Ekmeklik Buğday Çeşitlerinde Rapd ve Issr Analizleriyle Genetik Farklılıkların Belirlenmesi

The objective of this study was to determine genetic distance between some wheat varieties based on RAPD and ISSR analyses. The number of amplified bands of genotypes in the primers ranged 3-10 in RAPD and 6-12 in ISSR. The most polymorphic bands in primer/primer combinations were obtained from OPBA-03, OPL-15 and OPY-13 in RAPD, from UBC810 in ISSR. Varieties were classified about in three groups based on the dendogram results. Yunus and Altay-2000, Nacibey and Sultan-95, Harmankaya and Soyer were found to be close related varieties, whereas distant-related varieties were Alpu-01 and Nacibey. RAPD and ISSR are efficiently used to evaluate genetic variation, to determine genetic diversities and improvement and development of novel varieties.
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Süleyman Demirel Üniversitesi Ziraat fakültesi Dergisi-Cover
  • ISSN: 1304-9984
  • Yayın Aralığı: Yılda 2 Sayı
  • Yayıncı: Süleyman Demirel Üniversitesi Ziraat Fakültesi
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