TÜMAY ÖZGÜR, OĞUZHAN ÖZCAN, Seçkin AKKÜÇÜK, Erol KILIÇ, ONUR KOYUNCU, Cem ORUÇ, Akın AYDOĞAN, Ozan Utku ÖZTÜRK, Sedat MOTOR, İBRAHİM YETİM, Muhittin TEMİZ

Deneysel Kolit Modelinde Centella Asiatika Ekstresinin Etkinliği

Amaç: İnflamatuvar barsak hastalıkları (İBH) çevresel, genetik ve immün faktörlerin sebep olduğu düşünülen bir grup kronik ve inflamatuvar durum olarak tanımlanmaktadır. Tedavisinde başlıca antiinflamatuvar, immünsupresif ve sitotoksik ilaç kombinasyonları kullanılmaktadır. Fakat bu ajanların ciddi yan etkilerinden dolayı yeni tedavi yöntemleri bulmak için araştırmalar yapılmaktadır. Bu çalışmanın amacı dekstran sülfat sodium (DSS) ile indüklenmiş deneysel kolit modelinde Centella Asiatica (CA) ekstresinin etkinliğini araştırmaktır. Gereç ve Yöntem: Çalışmada 24 adet Wistar Albino rat üç eşit gruba ayrıldı. Grup 1' e (kontrol) sadece su verildi. Grup 2' ye (kolit) DSS' li içme suyu ve Grup 3'e (medikasyon grubu) 200mg/kg/gün CA ekstresi ve DSS' li içme suyu verildi. Deney sonunda kolonun histopatolojik incelemesi ve myeloperoksidaz (MPO), malonildialdehit (MDO), tümör nekroz factör alfa (TNF-?) ve interlökin-10 (IL-10) düzeyleri belirlendi. Bulgular: CA ekstratı veilen grupta kolit grubu ile kıyaslandığında TNF- ? düzeyi daha düşüktü ve İL 10 düzeyi daha yüksekti. MPO ve MDA düzeyleri kolit grubunda diğer gruplarla karşılaştırıldığında anlamlı düzeyde daha yüksek bulundu. (p

The Effectivity of Centella Asiatica Extract in Experimental Colitis Model Induced by Dextran Sulfate Sodium in Rats

Background: Inflammatory bowel disease (IBD) is a group of chronic and inflammatory conditions that are considered to be caused by environmental, genetic, and immune factors. The treatment of IBD mainly involves combinations of anti-inflammatory, immunosuppressive, and cytotoxic agents. However, due to serious side effects of these agents, research is being performed to find new treatment methods. The aim of this study is to investigate the efficacy of Centella asiatica (CA) in the experimental colitis model. Materials and Methods: In this study, twentyfour rats were divided into 3 equal groups; control group (group 1) received only water and standard rat chow; colitis group (group 2) received water with dextran sulfate sodium (DSS); medication group (group 3) received water with DSS, and 200 mg/kg/day CA extract was administered Histopathologic examination of colon, concentrations of myeloperoxidase (MPO), malondialdehyde (MDA), tumor necrosis factor alpha (TNF-?), and interleukin-10 (IL-10), were determined.Results: The results indicated that the TNF-? level was lower and the IL-10 was higher in the rats administered with CA extract compared to the rats in the colitis group (p<0.05). The MPO and MDA levels were significantly higher in the colitis group compared to other groups (p<0.05). Histopathologic examination revealed less tissue damage in the rats administered with CA extract. Conclusion: The CA extract exerts an immunoregulatory effect by altering the levels of anti- and pro-inflammatory cytokines in experimental IBD models. It also prevents tissue damage by inhibiting the formation of free oxygen radicals. Depending on these effects, we consider that the CA extract can be a useful agent in the treatment of IBD.

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