Semanur ÖZCAN, Cennet Gülnihal ŞEKER, Monira RAHİM, Şeyma KILIÇARSLAN, Rasih YAZKAN, Kadir ÇEVİKER

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Biyolojik damarsal greft üretiminde hücresizleştirme metodları; güncel literatür derlemesi

Hücresizleştirme (Decellularization) hücre ve hücre içi organellerin zarlarının bütünlüğünü bozarak DNA, RNA, yağlar, mitokondri ve sitoplazmik zardaki hücre kimliğini taşıyan proteinler ve sitozolik içerik gibi tüm hücresel yapıların temizlenerek, önemli hücre dışı matriks yapılarının üç boyutlu ultrastrüktür, temel matriks proteinleri ve büyüme faktörleri gibi öğelerinin korunması ve sonrasında implantasyon ile in vivo ya da in vitro ortamda dokunun kendine ait özelliklerle başarılı bir şekilde yeniden modellenmesi işlemidir. Bu derleme makalesinin amacı damar dokusunun hücresizleştirilmesi işlemi sırasında kullanılan birçok farklı çeşit yöntemin incelenerek başarılı veya başarısız yönlerinin ortaya konmasıdır.

The decellularization methodologies for the production of biologic vascular graft; an updated literature review

Decellularization is disrupting the integrity of cells and intracellular organelles' membranes, cleaning all of the cytosolic content and cellular structures that have the identity of the cells such as DNA, RNA, lipids, proteins and membranes, and preserving the important extracellular matrix structures such as three-dimensional ultrastructure, basic matrix proteins and growth factors. The successful decellularization may ensure a successful recellularization of the tissue after implantation in vivo or in vitro. The aim of this review article is inspecting successful or unsuccessful aspects of the different types of methods used during the decellularization process of the vascular tissue.
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Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi-Cover
  • ISSN: 1300-7416
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 1994
  • Yayıncı: SDÜ Basımevi / Isparta
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