Sevinç AKTEMUR TÜRKER, Emel UZUNOĞLU, Mehtap ASLAN, - -

Mineral trioksit aggregate içerikli kök kanal dolgu patının Enterococcus faecalise karşı antimikrobiyal etkinliğinin değerlendirilmesi

Amaç: Bu çalışmanın amacı, epoksi-resin (AH Plus) ve mineral trioksit aggregate esaslı (MTA Fillapex) kök kanal dolgu patlarının Enterococcus faecalise karşı antimikrobiyal etkinliklerinin karşılaştırılmasıdır. Materyal-Metod: Enterococcus faecalis izolatı triptik soy buyyon besiyerinde 12 saatlik kültüründen Mc Farland standart bulanıklık 0,5 (1.5x 10-8 CFU mL) eşeline denk süspansiyonu hazırlandı. Bu süspansiyondan kanlı agar besiyerlerine ekimler yapıldı. Hazırlanan kök kanal dolgu patları besiyerlerinde açılan çukurlara dolduruldu. 24, 48 ve 72. saat inkübasyon sürelerinin sonunda inhibisyon zonları ölçülerek kaydedildi. Veriler Eşleştirilmiş t testi ve Student's t testi kullanılarak istatistiksel olarak analiz edildi. Analizler % 95 güven aralığında gerçekleştirildi (p 0.05). Kök kanal dolgu patlarının inkübasyon sürelerindeki antimikrobiyal etkinlikleri arasında istatistiksel olarak anlamlı bir fark bulundu (p

Anahtar Kelimeler:

Agar difüzyon, AH Plus, antimikrobiyal etkinlik, MTA Fillapex

Evaluation of the antimicrobial activity of mineral trioxide aggregate-based root canal sealer against Enterococcus faecalis.

Aim: The aim of this study was to compare the antimicrobial properties of epoxy-resin (AH Plus) and mineral trioxide aggregate based root canal sealers (MTA Fillapex) against Enterococcus faecalis. Material &Method: The inocula of Enterococcus faecalis was prepared from 12 h tryptone soy broth and suspensions were adjusted to 0.5 Mc.Farland (1.5x 10-8 CFU/mL) standard turbidity. The inoculum was spread on the blood agar broths. The freshly mixed sealers were placed into the prepared wells on the agar plates. After incubation periods of 24, 48 and 72 h the zone of growth inhibition were measured and recorded. Data were analysed using paired samples t-test and Student's t test. The testing was performed at 95% confi¬dence level (p 0.05). There was a significant difference between root canal sealers with respect to antimicrobial activity in different incubation times (p

Keywords:

Agar diffusion, AH Plus, antimicrobial activity,

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Mohammadi Z. Chemomechanical strategies to manage endodontic infections. Dent Today. 2010; 29: 91-92.
Mohammadi Z, Giardino L, Palazzi F, Shalavi S. Antibacterial activity of a new mineral trioxide aggregate- based root canal sealer. Int Dent J. 2012; 62: 70-73.
Sundqvist G. Ecology of the root canal flora. J Endod. 1992; 18: 427-430.
Nair PN, Henry S, Cano V, Vera J. Microbial status of apical root canal system of human mandibular first molars with primary apical periodontitis after “one-visit” endodontic treatment. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005; 99: 231-252.
Morgental RD, Vier-Pelisser FV, Oliveira SD, Antunes FC, Cogo DM, Kopper PM. Antibacterial activity of two MTA- based root canal sealers. Int Endod J. 2011; 44: 1128-1133.
Siqueira JF Jr. Aetiology Of Root Canal Treatment Failure: Why Well-Treated Teeth Can Fail. Int Endod J. 2001; 34: 1-10.
Dentin extends the antibacterial effect of endodontic sealers against Enterococcus faecalisbiofilms. Wang Z, Shen Y, Haapasalo M. J Endod. 2014; 40: 505-508.
Antibacterial effect of different root canal sealers on three bacterial species. Heyder M, Kranz S, Völpel A, Pfister W, Watts DC, Jandt KD, Sigusch BW. Dent Mater. 2013; 29: 542-549.
Antibiofilm activity, pH and solubility of endodontic sealers. Faria-Jşnior NB, Tanomaru-Filho M, Berbert FL, Guerreiro-Tanomaru JM. Int Endod J. 2013; 46: 755-762.
Rocas In, Sıqueıra Jf, Jr., Santos Kr. Association Of Enterococcus Faecalis With Different Forms Of Periradicular Diseases. J Endod. 2004; 30: 315-320.
Lai CC, Huang FM, Yang HW, Chan Y, Huang MS, Chou MY, et al. Antimicrobial activity of four root canal sealers against endodontic pathogens. Clin Oral Investig. 2001; 5: 236-239.
Cobankara FK, Altinoz HC, Ergani O, Kav K, Belli S. In vitro antibacterial activities of root-canal sealers by using two different methods. J Endod. 2004; 30: 57-60.
Gomes BP, Pedroso JA, Jacinto RC, Vianna ME, Ferraz CC, Zaia AA, et al. In vitro evaluation of the antimicrobial activity of five root canal sealers. Braz Dent J. 2004; 15: 30- 35.
Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root-filled teeth with apical periodontitis. Int Endod J. 1998; 31: 1-7.
Madani ZS, Sefidgar SA, Rashed Mohasel A, Zabihi E, Mesgarani A, Bijani A, et al. Comparative evaluation of antimicrobial activity of two root canal sealers: MTA Fillapex and AH 26. Minerva Stomatol. 2014; 63: 267-272.
Bodrumlu E, Semiz M. Antibacterial activity of a new endodontic sealer against Enterococcus faecalis. J Can Dent Assoc. 2006; 72: 637.
Kayaoglu G, Erten H, Alacam T, Orstavik D. Short- term antibacterial activity of root canal sealers towards Enterococcus faecalis. Int Endod J. 2005; 38: 483-438.
Eldeniz AU, Erdemir A, Hadimli HH, Belli S, Erganis O. Assessment of antibacterial activity of EndoREZ. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006; 102: 119- 126.
Siqueira JF, Jr., Favieri A, Gahyva SM, Moraes SR, Lima KC, Lopes HP. Antimicrobial activity and flow rate of newer and established root canal sealers. J Endod. 2000; 26: 274- 277.
Miyagak DC, de Carvalho EM, Robazza CR, Chavasco JK, Levorato GL. In vitro evaluation of the antimicrobial activity of endodontic sealers. Braz Oral Res. 2006; 20: 303- 306.
Bodrumlu E, Avşar A, Eğilmez T. Üç farklı rezin esaslı kök kanal patının Antimikrobiyal etkinliğinin değerlendirilmesi. GÜ Diş Hek Fak Derg. 2008; 25: 19-22.
Zhou HM, Shen Y, Zheng W, Li L, Zheng YF, Haapasalo M. Physical properties of 5 root canal sealers. J Endod. 2013; 39:1281-1286.
Siqueira JF, Jr., de Uzeda M. Intracanal medicaments: evaluation of the antibacterial effects of chlorhexidine, metronidazole, and calcium hydroxide associated with three vehicles. J Endod. 1997; 23: 167-169.
Fraga RC, Siqueira JF, Jr., de Uzeda M. In vitro evaluation of antibacterial effects of photo-cured glass ionomer liners and dentin bonding agents during setting. J Prosthet Dent. 1996; 76: 483-486.