Kanat GÜLLE, İbrahim PALA, Meryem FERAH AKPOLAT, Bekir Hakan BAKKAL

İyonize Radyasyona Maruz Kalan Sıçan Ovaryumunda PARP-1 Ekspresyonu ve Follikülogenez Üzerine Curcuminin Koruyucu Etkisi

Amaç: Çalışmamızda, iyonize radyasyonun folliküleratrezi üzerine etkilerini göstererek, oluşacak hasarlara karşıcurcuminin koruyucu etkisinin olup olmadığını ve hücreçekirdeklerinde meydana gelen DNA hasarını tamir eden Poli(ADP-riboz)polimeraz-1 enziminin immünohistokimyasalboyama yöntemiyle gösterilmesi amaçlandı.Materyal-Method: Çalışmamız için seçilen dişi sıçanlar,biri kontrol olmak üzere toplam 4 gruba ayrıldı. Radyasyonhasarı oluşturmak amacıyla kontrol ve curcumin grubudışındaki deneklere tek doz tüm vücut 8.3 Gy iyonizeradyasyon uygulandı. Curcumin ve radyasyon+curcumingrubu deneklere; ışınlamadan 7 gün önce başlayarak günde100 mg/kg curcumin oral yoldan verildi. Işınlamadan sonraki4. günde deneklerden anestezi altında ovaryum dokularıalınarak mikroskobik incelemeler için işlemlendirildi.Bulgular: Radyasyon uygulamasından 4 gün sonra alınanovaryum dokularının kesitlerini incelediğimizde, ışınlanmışgruplardaki atretik özellik gösteren folliküller sayıcafazlaydı. Radyasyon ile ışınlanan curcumin tedavili grupta iseatretik follikül oranı radyasyon grubuna göre daha düşüktü.Curcumin ile tedavi edilen grupta, curcuminin radyasyonabağlı meydana gelen hasarları kısmen engellediği tespit edildi.Sonuç: Bu sonuçlar curcuminin ovaryum hasarında koruyucuetksi olduğunu göstermiştir.

Protective Effect of Curcumin on Folliculogenesis, and PARP-1 (Poly ADP-ribose polymerase) Expression Exposed İonising Raditation in Rat Ovary

Objective: : The aim of our study was to show the effects of ionising radiation on ovarium follicular atresia so that the protective effect of curcumin on the resulting damage could be determined, and in addition to immunohistochemically determining to level of expression of PARP-1 enzyme is responsible for DNA repair in the nucleus of the cells protected from radiation or irradiated the cells. Material-Method: The female rats chosen for the study were divided into 4 groups, one being the control group. The whole body of the rats in the other groups with the exception of those in the control group and the curcumin group was exposed to a single dosage of ionising radiation (8.3 Gy) with a view to causing radiation-induced damage. The animals in the curcumin and in the irradiated with treated curcumin had been fed a daily 100 mg/kg of curcumin through the intragastric tract for seven days in a row before the radiation process was launched. Ovarium tissues sample of these animals were collected for the purpose of microscopic examinations. Results: The ovarium tissues cultivated at the 4 day of the application of ionising radiation showed that there were the number of follicles showing atresic features was significantly high in the irradiated group. On the other hand, in the irradiated with treated curcumin group showed that the number of atretic follicles was lower than that in the irradiated group. The irradiated group given curcumin were observed to prevent the follicular damage caused by radiation. Conclusions: These results suggest that curcumin is protective effect in ovarian damage.

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