EMEL DEMİRBAĞ, KENAN ÇINAR, MEHMET ALİ TABUR, Resat Nuri ASTI

Histochemical Structure of Stomach (Proventriculus and Gizzard) in Some Bird Species

Bu çalışmada atmaca (Accipiter nisus), karga (Corvus corone) ve serçeden (Passer domesticus) alınan mide örnekleri (proventrikulus ve gizzard) incelendi. Histokimyasal incelemelerde, proventrikulusun bileşik bezlerinde güçlü sülfatlı musinlere rastlanmadı; fakat atmaca ve karga proventrikulusunda bileşik bezlerde AB (pH 0.5)e karşı zayıf bir reaksiyon gözlendi. Serçe gizzardında zayıf AB (pH 1.0) (+) karakter bulunurken, atmacanın superficial bezlerinde yoğundu. Bununla birlikte karga gizzardında bu karaktere rastlanmadı. AF(+) karakterin (sülfatlı musinler) atmaca gizzardının epitelinde yoğun, bezlerinde zayıf olduğu tespit edilirken; karga ve serçe gizzardlarının bezlerinde bu karakter gözlenmedi. Serçe proventrikulusunun hem epiteli hem de bezlerinde Periyodik Asit-Shiff (PAS) boyamasına karşı hiç bir reaksiyon gözlenmedi.

Bazı Kuş Türlerinde Mide (Proventrikulus ve Gizzard)nin Histokimyasal Yapısı

Stomach samples (proventriculus and gizzard) of sparrow hawk (Accipiter nisus), crow (Corvus corone) and sparrow (Passer domesticus) were investigated in this study. In histochemical investigations, strong sulfated mucins were not determined in compound glands of proventriculus, but a little reaction to AB (pH 0.5) in compound glands was observed in proventriculus of sparrow hawk and crow. While weak AB (pH 1.0) (+) character was found in sparrow gizzard, it was found as dense in superficial glands of sparrow hawk. However, it was not observed in crow gizzard. While AF (+) character (sulfated mucins) was detected as dense in epithelium and as weak in glands of gizzard of sparrow hawk, this character was not observed in glands of gizzards of crow and sparrow. Any reactivity to Periodic Acid-Shiff (PAS) staining was not observed in both epithelium and glands of sparrow proventriculus.

PDF

___

Allen, A. 1981. Structure and function of gastrointestinal mucus. Johnson L. (Editor), Physiology of the gastroenterology Tract, Raven Press, New York. 617-39 pp.
Altamirano, F., Avila, R., Samar, M.E., de Fabro, S.P. 1984. Cytochemical characterization of mucosubstances in the chick glandular stomach during embryonary and postnatal development. Folia Histochem Cytobiol, 22(2): 105-11.
Boat, T.F., Cheng, P.W. 1980. Biochemistry of airway mucus secretions. Fed. Proc., 39 (13): 3067-74.
Chikilian, M., De Speroni, N.B. 1996. Comparative study of the digestive system of three species of tinamou. I. Crypturellus tataupa, Nothoprocta cinerascens, and Nothura maculosa (Aves: Tinamidae). J. Morphol., 228 (1): 77-88.
Gomari, G. 1952. Gomaris aldehyde fuchsin stain. Culling CFA, Allison RT. and Barr WT (Editors.): Cellular Pathology Tecnique, Butterworths, London. 238 pp.
Imai, M., Shibata, T., Moriguchi, K., Yamamoto, M., Hayama, H. 1991. Proventricular glands in fowl. Okajimas Folia Anat Jpn, 68 (2-3): 155-160.
Inforzato de Lima, M.A., Sasso Wda, S. 1985. Histochemical detection of glycoproteins in the gastric epithelia of Columba livia. Acta Histochem, 76 (2):145-150.
Jamroz, D., Wertelecki, T., Houszka, M., Kamel, C. 2006. Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejenum walls in chicken. J Anim Physiol Anim Nutr (Berl), 90 (5-6): 255-68.
Jones, R. 1977. Modification of mucus in animal models of disease. Proceedings of International Symposium on Mucus in Health and Disease. Raven Pres, New York. 63-72 pp.
Lev, R., Spicer, S.S. 1964. Specific staining of sulfate groups with alcian blue at low pH. J. Histohem. Cytochem, 12: 309.
Liman, N., Alan, E., Küçük Bayram, G. 2010. The differences between the localizations of MUC1, MUC5AC, MUC6 and osteopontin in quail proventriculus and gizzard may be a reflection of functional differences of stomach parts. Journal of Anatomy, 217: 57-66.
Linden, S.K., Sutton, P., Karlsson, N.G., Korolik, V., McGuckin, M.A. 2008. Mucins in the mucosal barrier to infection. Mucosal Immunology, 1: 183 197.
McManus, J.F.A. 1948. Histological and histochemical uses of periodic acid. Stain Technol, 23: 99-108.
Mowry, R.W. 1956. Alcian blue tecniques for the histochemical study of acidic carbohydrates. J. Histochem. Cytochem, 4: 407-408.
Neutra, M., Forstner, J. 1987. Gastrointestinal mucus: synthesis, secretion, and function. Johnson L (Editor): Physiology of the Gastrointestinal Tract, Chapter 34. Raven Pres, New York.
Pastor, L.M., Ballesta, J., Madrid, J.F., Perez-Thomas, R., Hernandez, F. 1988. A histochemical study of the mucins in the digestive tract of the chicken. Acta Histochem, 83: 91-97.
Selvan, P.S., Ushakumary, S., Ramesh, G. 2008. Studies on the histochemistry of the proventriculus and gizzard of post-hatch guinea fowl (Numida meleagris). International Journal of Poultry Science, 7 (11): 1112-1116.
Shyla, P., Ommer, P.A., Lucy, P. 1992. Structure and post-natal development of the proventriculus in the duck. Ind J Poult Sci, 27: 10-14.
Spicer, S.S., Mayer, D.R. 1960. Aldehyde fuchsin/alcian blue. Culling CFA, Allison RT and Barr WT (Editors): Cellular Pathology Tecnique, Butterworths, London. 233 pp.
Straus, G.J., Dekker, J. 1992. Mucin-type glycoproteins. Crit Rev Biochem Mol Biol, 27 (1-2): 57-92.