Hüseyin KAHRAMAN, Emel AYTAN, Aslı GİRAY KURT, Duygu ÖZCAN

Hemoglobin Geni Taşıyan Rekombinant Erwinia herbicola’da Ağır Metal Varlığında Fenazin Üretimi

Bu çalışmada, Vitreoscilla sp. elde edilen bakteriyel hemoglobin (VHb) geni (vgb) aktarılmış yabanıl Erwinia herbicola ve rekombinat suş Eh [pUC8:15] da ağır metal varlığında fenazin üretimi araştırılmıştır. Yabanıl bakteri, zamana bağlıolarak fenazin üretiminde önemli bir değişim göstermemiştir. Genel olarak, Pb varlığında yabanıl bakteri, 96 saatin sonunda 2.91 mg/ml fenazin üretirken rekombinant bakteride 9.5 mg/ml dir. Fenazin üretimi en yüksek değerlere rekombinant bakteride sırasıyla, 7, 8.8, 8 ve 9.5 mg /ml olacak şekilde Cd, Co, Fe ve Pb varlığında gerçekleşirken; yabanıl bakteride; 6.32, 3.33, 6.02 ve 2.99 mg /ml olacak şekilde Cd, Co, Fe ve Pb varlığında gerçekleşmiştir. Genetik mühendisliği ile vgb, fenazin üretimi artırmak için etkili bir yöntem olabilir.

Anahtar Kelimeler:

Sekonder metabolizma, Erwinia herbicola, fenazin pigmenti, Vitreoscilla hemoglobin

Phenazine Production in The Presence of Heavy Metals in Recombinant Erwinia herbicola Bearing the Hemoglobin Gene

In this study, from Vitreoscilla sp. recombinant strains were obtained from cloned bacterial hemoglobin (VHb) gene (vgb) Eh [pUC8:15] and its wild-type strain Erwinia herbicola phenazine production in the presence of heavy metals were investigated. Time-dependent production of phenazine in the wild bacteria not shows a significant change. In generally, at the end of 96 hours in the presence of Pb in terms of production of wild-type bacteria phenazine 2.91 μg/ml of recombinant bacteria is to produce fenazin production 9.5 μg /ml, respectively. The average phenazine highest values were 7, 8.8, 8 and 9.5 μg/ml Cd, Co, Fe and Pb for Eh [pUC8:15]. E. herbicola is 6.32, 3.33, 6.02 and 2.99 μg/ml Cd, Co, Fe and Pb, respectively. Genetic engineering vgb, may be an effective method to increase phenazine production.

Keywords:

Seconder metabolism, Erwinia herbicola, phenazine pigment, Vitreoscilla hemoglobin,

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