Batı Akdeniz Sahil Kuşağından Toplanan Yonca (Medicago sativa L.) Populasyonlarının Moleküler Karakterizasyonu

Medicago tür ve populasyonlarında moleküler markırlar kullanılarakgenotipik varyasyon seviyesini belirlemek yaygın olarak kullanılmaktadır. Buçalışmada; Batı Akdeniz sahil kuşağında yer alan, Antalya iline ait 13 ilçede, 26farklı lokasyonda, doğal vejetasyondan toplanan üstün yonca genotipleri klon yöntemi ile çoğaltılmıştır. Elde edilen 26 genotip ve 2 standart çeşidinaralarındaki benzerlikler, Basit Tekrarlı Sekanslar (SSR) moleküler analiz tekniğiyle, 15 adet primer kullanılarak belirlenmiştir. SSR primerlerinin % 99,7'si polimorfik bant vermiştir. Araştırma sonucunda; kümeleme analizi sonucuoluşan dendograma göre yonca genotipleri 2 ana ve 4 alt gruba ayrılmıştır.Birinci ana grup % 60, ikinci ana grup ise % 65 seviyesinde kendi içerisinde iki alt gruba ayrılmıştır. Benzerlik katsayıları 0.58-0.88 değerleri arasında değişimgöstermiştir. En yakın genetik benzerlik Kaş-2 ve Çeşit-1 arasında bulunurken, en uzak ise Gazipaşa-1-Kumluca-2 genotipleri arasında bulunmuştur. Elde edilen bu sonuca göre Batı Akdeniz sahil kuşağından toplanan yonca genotipleriarasında genetik olarak önemli bir varyasyonun olduğu tespit edilmiştir

Molecular Characterization of Alfalfa(Medicago sativa L.) Populations Collected From West Mediterranean Coastal Zone

Molecular markers are widely used to determine the level of genetypicvariation in Medicago species and populations. In this study, superior alfalfagenotypes collected from 26 different locations of 13 districts of Antalyaprovince in the Western Mediterranean Coastal Zone were reproduced via clonalmethod. 15 different SSR primers were used to determine the similarities amonggenotypes at molecular level. SSR primers yielded polymorphic bands at a rate of99.7 %. According to research results; alfalfa genotypes were divided into 2 maingroups and 4 sub-clusters. In the first main group genotypes were divided intotwo sub-clusters at 60 % similarity level while second group were divide two sub-clusters at 65 % level. Similarity coefficient changed between 0.58 and 0.88among genotypes. The nearest genetic similarity was detected between Kas-2 and Variety-1. On the other hand, Gazipaşa-1 and Kumluca-2 genotypes were found to be the most distant genotypes. The results of the study revealed a widegenetic variation in the genotypes collected from Western Mediterranean CoastalZone

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Brummer, E. C. 1998. Defining heterotic groups in alfalfa: A molecular marker perspective. Proceedings of the 36th North American Alfalfa Improvement Conference. Bozeman, Montana August 2-6-1998
Brummer, E.C., Bouton J.H., Kochert, G. 1995. Analysis of annual Medicago species using RAPD markers. Genome, 38; 362-367.
Carelli, M., Gnocchi, G., Scotti, C. 2009. Alfalfa germ plasmfrom a Saharaoasis: characterization by means of bio-agronomic trait sand SSR markers. Plant Breeding 128, 271--277 (2009) doi:10.1111/j.1439-0523.2008.01573.x, Berlin
Diwan, N., Bouton, J.H., Kochert, G., Cregan, P.B. 2000. Mapping of Simple sequencerepeat (SSR) DNA markers in diploid and tetraploid alfalfa. Theor ApplGenet 101:165-172.
Doyle, J.J., Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12, 13-15.
Elçi, Ş. 2005. Baklagil ve Buğdaygil Yem Bitkileri. Mart Matbası, ISBN 975-407-189-6, Ankara.
Falahati-Anbaran, M.,Habashi, A. A., Esfahany, M., Mohammadi, S. A., Ghareyazie, B. 2007. Population genetic structure based on SSR markers in alfalfa (Medicago sativa L.) from various region scontiguousto the centres of origin of the species. Journal of Genetics, Vol. 86, No. 1, April 2007
Flajoulot, S.,Ronfort, J., Baudouin, P., Barre, P., Huguet, T., Huyghe, C., Julier, B. 2005. Genetic diversity among alfalfa (Medicago sativa) cultivars coming from a breeding program, using SSR Genetetics, 111, 1420-1429. and Applied
Habibi, B., Farshadfar, M., Safari, H. 2012. Evaluation of Genetic Diversity in 18 Genotypes of Alfalfa (Medicago Sativa) Using of Molecular ISSR Markers. International Journal of Agriculture and Crop Sciences. Available online at www.ijagcs.com IJACS/2012/4-21/1573- 1578. ISSN 2227-670X ©2012 IJACS Journal
Harlan, J. R. 1971. Agricultural Origins Centre and non-centers. Science, 174, 468- 474.
Karaca, M., Saha, S., Zipf, A., Jenkins, J.N., Lang, D.J. 2002. Genetic diversity among forage bermudagrass (Cynodon spp.). Evidence from chloroplast and nuclear DNA finger printing. Crop Science 42: 2118- 2127
Keivani, M., Ramezanpour, S. S., Soltanloo, H., Choukan, Rajab, Naghavi, M., Ranjbar, Mojtaba. 2010. Genetic diversity assessment of alfalfa (Medicago sativa L.) populations using AFLP markers Australian Journal of Crop Sicience AJCS 4(7):491-497 (2010) ISSN:1835-2707
Mckersie, B., Bowley, S. 1993. Synthetic seeds of alfalfa. In: Redenbaugh K (ed) Synseeds: Applications of Synthetic Seeds to Crop Improvement CRC Press, Boca Raton, pp. 231- 256.
Manga, Ü., Acar, Z., Ayan, İ. 1995. Baklagil Yem Bitkileri. Ondokuz Mayıs Üniversitesi Ziraat Fakültesi Ders Notu No 7.
Mengoni, A., Gori, A., Bazzicalupo, M. 2000. Use of RAPD and microsatellite (SSR) variationto ases genetic relationships among populations of tetraploidalfalfa, Breeding, 119, 311-317. sativa. Plant on morphological traits. Plant
Poehlman, J.M.1983. Breeding Field Crops. Second edition. The AVI Publishing Company, Inc, Westport, Connecticut. Second Printing.
Qiang, H., Chen, Z., Zhang, Z., Wang, X., Gao., H., Wang, Z. 2015. Molecular diversity and population structure of a worldwide collection of cultivated tetraploid alfalfa (Medicago sativa subsp.sativa L.)germplasm as revealed by micro satellite 22;10(4):e0124592.doi:10.1371/journal.pone.0 124592. PLoSOne. 2015 Apr
Rohlf, F.J. 1991. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. Exeter Software, Setauket, NY, USA.
Sledge, M. K., Bouton, J. H., Kochert, G. 1998. Molecular marker diversity as a means of selecting parents for synthetic cultivars. Proceedings of the 36th North American Alfalfa Improvement Conference. Bozeman, Montana, August 2-6, 1998.
Sneath, P.H.A., Sokal, R.R. 1973. Numerical taxonomy. W.H. Freman Co., San Francisco, 573s, USA.
Touil, L., Guesmi, F., Fares, K., Zagrouba, C., Ferchichi, A. 2008. Genetic diversity of some mediterranean populations of the cultivated alfalfa (Medicago sativa L.) using SSR markers. Pakistan Journal of Biological Science, 11 (15), 1923-1928.
Tucak, M., Popovic, S., Cupic, T., Simic, G., Gantner, R., Meglic, V. 2009. Evaluation of alfalfa germplasm collection by multivariate analysis based on Agricultural Research, 26: 47-52. traits. Romanian
Tucak, M.,Popovic, S., Bolaric, S., Kozumplik, V. 2008. Agronomic evaluation of alfalfa genotypes under ecological comditions of eastern Croatia. VII. Alps-Adria Scientific Workshop, Cereal Research Communications, 36, 651- 654.
Tuğay, E. 1996. Genel Bitki Islahı. Gazi Osman Paşa Üniversitesi Ziraat Fakültesi. Ders Notları s. 223 Tokat.
Ulukapı, K., Onus, A.N. 2013. Selekte Edilmiş Bazı Yerel Taze Fasulye (Phaseolus vulgaris L.) Genotiplerinin Moleküler Karakterizasyonu. Tarım Bilimleri Dergisi 18, 277-286.