Spirulina platensis (Cyanophyceae) mikroalg'inin büyümesine ve bazı biyokimyasal özelliklerine sıcaklığın etkisi

Mavi-yeşil alg Spirulina platensis (=Arthrospira), yoğun üretime uygun, fitoplanktonik bir organizmadır. Protein, karetenoid, fikosiyanin ve klorofil gibi pigmentleri, vitamin ve yağlan içerdiğinden, insan gıdası ve beslemede alternatif bir gıda olarak kullanılır. Çalışmada, laboratuar koşullarında S. platensis'in 2000 lux. ışık şiddetinde ve 28°C'den 45°C'ye kadar olan sıcaklıklarda büyümesi ve metabolizmanın etkilenmesi araştırıldı. Sıcaklık arttırılarak filamentlerin morfolojisindeki, büyümesindeki, makromoleküler yapısındaki, yağ ve yağ asitleri içeriklerindeki değişimler araştırıldı. Su sıcaklığı 43 °C olduğunda, protein içeriğinde %20 gibi önemli bir azalma, yağlarda %45 ve karbonhidratta %32 artma gözlendi. 5. platensis'in yoğun üretiminde biyokimyasal özelliklerinin ve büyümenin 35°C sıcaklıkta en uygun olduğu saptandı.

Anahtar Kelimeler:

algler, Spirulina, sucul bitkiler, çevresel faktörler, büyüme, sıcaklık, biyokimyasal parametreler

The effects of temperature on growth and some biochemical characteristics of microalgae spirulina platensis (Cyanophyceae)

Blue-green algae Spirulina platensis (Arthrospira) have found world-wide interest as photosynthetic planktonic organism suitable for mass production. It can be an alternative source of protein for human food and feed purposes, with the possibility of obtaining other products like pigments (carotenoids, phycocyanin and chlorophyll), vitamins and lipids. The biomass can be introduced directly in the diet and it can also be used in case of malnutration. In this study, S. platensis was grown in continuous culture under constant light (2000 lux), and temperatures to examine how its metabolism was influenced by high growth temperatures between 28°C to 45°C at laboratory system. Elevated temperatures determined changes in filament morfology, growth rates, macromolecular composition, lipid and fatty acid composition. At 43°C a significant decrease in the protein content (20%), a considerable increase of lipids (45%) and carbonhydrates (32%) were observed. Finally, the biochemical characteristics and growth of S. platensis was relatively stable up to 35°C and this indicate that 5. platensis requires for its mass production

Keywords:

algae, Spirulina, aquatic plants, environmental factors, growth, temperature, biochemical parameters,

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AOAC, 1990. Official Methods of Analysis of the Association of Official Analytical Chemists, 15 th. Edition, (Ed) Williams, S., Arlington, Virginia.

Bling, E.G., Dyer, W.J., 1959. A rapid method of total lipid extraction and purification, Canadian Journal Biochemistry Physiol., 37:911-1015.

Borowitzka, M:A., 1992. Vitamins and fine chemicals from micro-algae, In: Borowitzka, M.A., Borowitzka, L.J.(Eds), Micro-Algal Biotechnology, Cambridge University Pres, UK, 158-195 p.

Becker, E.W., 1995. Microalgae: Biotechnology and microbiology, Cambridge University Press, UK, 293 p.,

Cohen, Z., 1997. The Chemicals of Spirulina, p. 175-204. In: Vonshak, A (Ed.) Spirulina platensis (Arthrospira): Physiology, Cell-biology and Biotechnology, Taylor& Francis Ltd.

Dubois, M., Gilles, K.A., Hamilton, J.K., Rebes, P.A., Smith, F., 1956. Colorimetric method for determination of sugars and related substances, Analytical Chemistry 28, 350-355.

Fox, D.R., 1996. Spirulina: Production and Potential, 232 p., Edisud-France.

IUPAC, 1987. Standart Methods for Analysis of Oils, Fats and Derivatives, IUPAC Method 2.301, In: International Union of Pure and Applied Chemistry, 7th Ed. Blackwell Scientific Publications

Koru, E., Cirik, S., 2002. Biochemical Composition of Spirulina Biomass In Open-Air System, 1st International Congress on the Chemistry of Natural Products 16-19 October, ICNP 2002, Trabzon.

Sarada, R., Pillai, M.G., Ravishankar, G.A., 1998. Phycocyanin from Spirulina sp: Influence of Processing of Biomass on Phycocyanin Yield, Analysis of Efficiacy of Extraction Methods and Stability Studies on Phycocyanin, Process Biochemistry, 34:795-801.